##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547695_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2219929
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.661526562336004	31.0	31.0	33.0	30.0	34.0
2	32.03087576224284	33.0	31.0	34.0	30.0	34.0
3	32.09543728650781	33.0	31.0	34.0	30.0	34.0
4	35.80548071582469	37.0	35.0	37.0	35.0	37.0
5	35.73180853982267	37.0	35.0	37.0	33.0	37.0
6	35.78777924879579	37.0	35.0	37.0	35.0	37.0
7	36.12115702799504	37.0	35.0	37.0	35.0	37.0
8	36.01017915437836	37.0	35.0	37.0	35.0	37.0
9	37.83502175069563	39.0	38.0	39.0	35.0	39.0
10	37.46559056618477	39.0	37.0	39.0	35.0	39.0
11	37.09966534965758	39.0	37.0	39.0	33.0	39.0
12	36.0624610066358	37.0	35.0	39.0	33.0	39.0
13	35.66074725813303	37.0	35.0	39.0	31.0	39.0
14	36.52429649777088	38.0	35.0	41.0	31.0	41.0
15	36.817549119814196	38.0	35.0	41.0	32.0	41.0
16	37.00230097449062	38.0	35.0	41.0	33.0	41.0
17	36.9109273314597	38.0	35.0	41.0	32.0	41.0
18	36.83826825092154	38.0	35.0	41.0	32.0	41.0
19	36.812360215123995	37.0	35.0	41.0	32.0	41.0
20	36.686718359010584	37.0	35.0	41.0	32.0	41.0
21	36.52306222406212	37.0	35.0	41.0	32.0	41.0
22	36.44516874188319	37.0	35.0	40.0	32.0	41.0
23	36.41631331452492	37.0	35.0	40.0	32.0	41.0
24	36.39477929249089	37.0	35.0	40.0	32.0	41.0
25	36.32797760649102	37.0	35.0	40.0	32.0	41.0
26	36.26371474042638	37.0	35.0	40.0	32.0	41.0
27	36.17693313614985	37.0	35.0	40.0	31.0	41.0
28	36.19740631344516	37.0	35.0	40.0	31.0	41.0
29	36.184599597554694	36.0	35.0	40.0	31.0	41.0
30	36.2107175499757	36.0	35.0	40.0	31.0	41.0
31	36.02915994160173	36.0	35.0	40.0	31.0	41.0
32	35.79864040696797	36.0	35.0	40.0	30.0	41.0
33	35.71418410228435	36.0	35.0	40.0	30.0	41.0
34	35.55591327470383	36.0	35.0	40.0	30.0	41.0
35	35.46699331375012	36.0	35.0	40.0	30.0	41.0
36	35.36798924650293	36.0	35.0	40.0	29.0	41.0
37	35.087349640461476	36.0	34.0	40.0	27.0	41.0
38	35.21040763015394	36.0	34.0	40.0	29.0	41.0
39	35.16096235510235	36.0	34.0	40.0	29.0	41.0
40	35.025967497158696	36.0	34.0	40.0	28.0	41.0
41	35.00015180665688	36.0	34.0	40.0	28.0	41.0
42	35.016008620095505	36.0	34.0	40.0	28.0	41.0
43	34.912676036035386	35.0	34.0	40.0	27.0	41.0
44	34.76506500883587	35.0	34.0	40.0	27.0	41.0
45	34.7565102307326	35.0	34.0	40.0	27.0	41.0
46	34.60719194172426	35.0	34.0	40.0	27.0	41.0
47	34.47634721651008	35.0	34.0	40.0	26.0	41.0
48	34.38181040925183	35.0	34.0	40.0	26.0	41.0
49	34.47292999010329	35.0	34.0	40.0	27.0	41.0
50	34.32528472757462	35.0	34.0	40.0	26.0	41.0
51	33.70700324199558	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	8.0
12	7.0
13	15.0
14	35.0
15	80.0
16	168.0
17	372.0
18	906.0
19	1778.0
20	3177.0
21	4963.0
22	7512.0
23	10937.0
24	15763.0
25	22766.0
26	30699.0
27	36496.0
28	39806.0
29	43697.0
30	51336.0
31	62617.0
32	80244.0
33	108243.0
34	218027.0
35	408695.0
36	124135.0
37	163105.0
38	262220.0
39	521976.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.45365054467958	23.268717152665694	29.877712305213365	14.39991999744136
2	30.50007455193387	24.88831850027636	31.001577077465093	13.61002987032468
3	28.74497337527461	24.699483632134182	32.6700989085687	13.885444084022508
4	26.727386326319447	27.06036994876863	31.16478049523205	15.04746322967987
5	23.87148417809759	31.32469551954139	29.991409635173017	14.812410667188006
6	22.601488606167134	39.87262655697547	26.811668301103325	10.71421653575407
7	88.01146342968626	3.9570184451845085	6.469846558155688	1.5616715669735384
8	89.49961913196323	3.0761344169115317	5.722750592473903	1.7014958586513351
9	86.56538114507266	4.218017783451633	6.869859351357634	2.3467417201180756
10	55.515469188429	23.943017997422437	11.761637421737362	8.7798753924112
11	48.555877237515254	18.328694296078837	19.971900002207278	13.143528464198631
12	44.1869987733842	20.413806027129695	21.958179743586395	13.441015455899716
13	24.431276856151708	38.46208594959569	22.164762927102625	14.941874267149984
14	15.83235319688152	41.511147428588934	27.235465638765927	15.42103373576362
15	14.97520866658348	25.130082989140647	47.06384753746629	12.830860806809588
16	18.90015401393468	19.49256034765076	45.23405928748171	16.373226350932846
17	19.187865918234323	19.471793917733404	28.177072329790725	33.163267834241545
18	23.615935464602696	24.354968109340433	33.11015802757656	18.91893839848031
19	29.237331464204487	26.043580673075578	25.667352424334293	19.05173543838564
20	32.748930258580344	25.05408956772942	24.442178105696176	17.754802067994067
21	24.55515469188429	27.06059518119724	28.36320440878965	20.021045718128825
22	23.6380983355774	22.274586259290274	28.66627716472013	25.42103824041219
23	19.772389116949235	27.693453259090717	27.30235967006152	25.23179795389853
24	21.57451882470115	23.995857525173104	37.40344848866788	17.026175161457864
25	18.722986185594223	24.212981586348032	35.003101450541884	22.060930777515857
26	17.133430843959424	34.40096507591009	27.52597042518026	20.939633654950228
27	17.539389773276532	33.874326611346575	30.856842718843712	17.729440896533177
28	14.937549804520774	28.953538604162567	38.45541906970899	17.65349252160767
29	16.319395800496324	25.61104431718312	37.84247153850416	20.2270883438164
30	20.09622830279707	29.611487574602613	32.61879096133254	17.67349316126777
31	30.832067151697196	25.501671449852676	25.490770200308212	18.17549119814192
32	30.09190834481643	27.050910186767236	26.809010558445788	16.048170909970544
33	28.617897239055846	27.80751096093614	25.18886865300647	18.38572314700155
34	20.561198128408613	29.187149679111357	27.455382582055556	22.796269610424478
35	19.615582300154642	27.49218556088956	30.659539111385996	22.2326930275698
36	29.711896191274583	24.616237726521884	27.862422627029964	17.809443455173565
37	21.091980869658443	32.05629549413517	28.945835655104286	17.905887981102097
38	20.75039336843656	32.603520202673145	23.610079421458973	23.03600700743132
39	20.182222044038344	31.127797330455167	28.010760704509018	20.679219920997475
40	23.934188886221136	26.678015377969295	25.280898623334352	24.10689711247522
41	18.516718327478042	23.45930883375099	28.858670705234267	29.165302133536702
42	22.58306459350727	24.730745893224512	25.034179021040764	27.652010492227454
43	23.28434828321086	24.297398700589074	26.284399185739726	26.133853830460346
44	19.376385460976454	29.909605217103792	28.33000514881332	22.384004173106437
45	17.90800516593098	36.797348023292635	23.679360916497778	21.615285894278603
46	23.831077480405906	31.19041194560727	25.041476551727555	19.93703402225927
47	21.22639958304973	29.577207198968974	26.08759108962494	23.108802128356356
48	23.499985810357	24.648581103269517	30.192677333374174	21.658755752999305
49	21.82813053931004	23.92234166047653	31.579568535750468	22.669959264462964
50	20.10767011017019	30.847518096299474	28.057518956687353	20.98729283684298
51	18.72429253368013	33.614318295765315	25.483517716107134	22.177871454447416
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1638.0
1	2581.5
2	3525.0
3	10483.0
4	17441.0
5	12819.5
6	8198.0
7	8545.5
8	8893.0
9	9354.0
10	9815.0
11	9761.5
12	9708.0
13	9466.5
14	9225.0
15	8353.0
16	7481.0
17	7710.5
18	7940.0
19	7086.5
20	6233.0
21	6416.5
22	6600.0
23	7707.5
24	8815.0
25	9976.5
26	13448.5
27	15759.0
28	18439.5
29	21120.0
30	23686.0
31	26252.0
32	33884.5
33	41517.0
34	45565.0
35	49613.0
36	54333.0
37	59053.0
38	74183.0
39	89313.0
40	128648.0
41	167983.0
42	196084.5
43	224186.0
44	229583.5
45	234981.0
46	222741.5
47	210502.0
48	195408.0
49	180314.0
50	166785.5
51	153257.0
52	143543.5
53	133830.0
54	118372.0
55	102914.0
56	98017.0
57	93120.0
58	89902.0
59	86684.0
60	78851.0
61	71018.0
62	62086.5
63	53155.0
64	44410.5
65	35666.0
66	27571.5
67	19477.0
68	16175.5
69	12874.0
70	10887.0
71	8900.0
72	6841.5
73	4783.0
74	4216.5
75	2821.0
76	1992.0
77	1359.0
78	726.0
79	535.5
80	345.0
81	223.0
82	101.0
83	120.5
84	140.0
85	78.5
86	17.0
87	13.0
88	9.0
89	14.0
90	19.0
91	11.5
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2219929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.620106499886354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.34018804600457	13.851228010451516
2	7.0588026906441055	2.3463610496037877
3	1.934187983432847	0.9643923082636301
4	0.873121322424416	0.5804547746406161
5	0.5225643429721677	0.4342537516620283
6	0.38104508853826324	0.3799805971658736
7	0.24693191107245924	0.28728242621713174
8	0.2101811118644561	0.279458597076143
9	0.17142529536830695	0.2564195999216162
>10	2.1001446760075004	8.67406046277932
>50	1.1669506338075961	14.22564069910626
>100	1.9841130515253393	55.99737347792062
>500	0.008710607442505148	0.8904335057594085
>1k	0.0013610324128914295	0.3913347131805143
>5k	2.7220648257828586E-4	0.44132602625155115
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9755	0.43942846820776704	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3912	0.17622185214031622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020180825602980998	0.0
2	0.0	0.0	0.0	0.06910130909592153	0.0
3	0.0	0.0	0.0	0.09576882864271785	0.0
4	0.0	0.0	0.0	0.15347337685124163	0.0
5	0.0	0.0	0.0	0.2688374267825683	0.0
6	0.0	0.0	0.0	0.39239993711510596	0.0
7	0.0	0.0	0.0	0.47609630758461197	0.0
8	0.0	0.0	0.0	0.6599310158117669	0.0
9	0.0	0.0	0.0	0.718896865620477	0.0
10	0.0	0.0	0.0	0.8249362930075692	0.0
11	0.0	0.0	0.0	0.9697607446003904	0.0
12	0.0	0.0	0.0	1.0894042106752062	0.0
13	0.0	0.0	0.0	1.136703020682193	0.0
14	0.0	0.0	0.0	1.1521539652844752	0.0
15	0.0	0.0	0.0	1.1809837161458767	0.0
16	0.0	0.0	0.0	1.245895702069751	0.0
17	0.0	0.0	0.0	1.3270694693388843	0.0
18	0.0	0.0	0.0	1.4369828944979772	0.0
19	0.0	0.0	0.0	1.4968496740211061	0.0
20	0.0	0.0	0.0	1.5599598005161426	0.0
21	0.0	0.0	0.0	1.652305096244069	0.0
22	0.0	0.0	0.0	1.7469477627437635	0.0
23	4.504648572093972E-5	0.0	0.0	1.8569963273600192	0.0
24	4.504648572093972E-5	0.0	0.0	1.9367286070860825	0.0
25	4.504648572093972E-5	0.0	0.0	2.0045235680960967	0.0
26	4.504648572093972E-5	0.0	0.0	2.0772285960496935	0.0
27	4.504648572093972E-5	0.0	0.0	2.143627116002359	0.0
28	4.504648572093972E-5	0.0	0.0	2.218179049870514	0.0
29	4.504648572093972E-5	0.0	0.0	2.3079116494266256	0.0
30	4.504648572093972E-5	0.0	0.0	2.4141763092423227	0.0
31	4.504648572093972E-5	0.0	0.0	2.517873319371926	0.0
32	4.504648572093972E-5	0.0	0.0	2.6072455470422704	0.0
33	4.504648572093972E-5	0.0	0.0	2.703825212427965	0.0
34	4.504648572093972E-5	0.0	0.0	2.80472934044287	0.0
35	4.504648572093972E-5	0.0	0.0	2.934057800947688	0.0
36	4.504648572093972E-5	0.0	0.0	3.0369439743343145	0.0
37	9.009297144187944E-5	0.0	0.0	3.1479835616364307	0.0
38	9.009297144187944E-5	0.0	0.0	3.274924558398039	0.0
39	9.009297144187944E-5	0.0	0.0	3.4779490695423143	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAAT	35	1.2126111E-7	45.000004	11
TCGTTAG	20	7.03471E-4	45.0	1
TAGACGT	25	3.891984E-5	45.0	27
CGTGATA	55	1.8189894E-12	45.0	39
CGTTGAC	40	6.8193913E-9	45.0	19
CCGCTAT	20	7.03471E-4	45.0	38
CGTTACA	20	7.03471E-4	45.0	13
CTAACCG	25	3.891984E-5	45.0	23
CTCGATA	40	6.8193913E-9	45.0	24
CGTAAGA	25	3.891984E-5	45.0	12
TATAGCG	55	1.8189894E-12	45.0	1
CAGTCGA	20	7.03471E-4	45.0	1
TGAATCG	20	7.03471E-4	45.0	13
GTAACGA	20	7.03471E-4	45.0	20
AATCGAT	20	7.03471E-4	45.0	20
AGTCGAC	25	3.891984E-5	45.0	42
TAACGCG	30	2.16642E-6	44.999996	18
CACGTAT	30	2.16642E-6	44.999996	36
CGTTTTT	7190	0.0	42.715576	1
CTTGACG	165	0.0	42.272728	1
>>END_MODULE