FastQCFastQC Report
Thu 26 May 2016
SRR1547694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547694_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3124517
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119660.38297119202743973No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC69760.22326650807148754No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT41100.1315403308735398No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA253.8923507E-545.08
ACGTTAC207.035151E-445.035
CGTAGAT253.8923507E-545.021
ACGTACT207.035151E-445.019
TATACGA207.035151E-445.012
TATCCGT406.8212103E-945.020
GTCGAAT302.1667056E-644.99999638
CCGCGTA302.1667056E-644.99999619
TATAGCG2550.043.2352941
TACGTAG1150.043.0434761
AACGTTC950.042.6315840
CGTTAGG4400.041.9318162
CGGTCTA3350.040.9701531
CGTTTTT89850.040.968281
ATACGTT501.0822987E-940.512
ATAGGGC13950.040.3225824
TAGCATA9850.040.20304530
CGTTTCA451.9301297E-840.033
ACGAACT451.9301297E-840.020
CGAATAT5100.039.70588314
Produced by FastQC (version 0.11.5)