##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547694_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3124517
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.419572689154837	31.0	31.0	33.0	30.0	34.0
2	31.82653478921702	31.0	31.0	34.0	30.0	34.0
3	31.97174731326474	33.0	31.0	34.0	30.0	34.0
4	35.6469700756949	37.0	35.0	37.0	33.0	37.0
5	35.52031081924022	37.0	35.0	37.0	33.0	37.0
6	35.57273556200846	37.0	35.0	37.0	33.0	37.0
7	35.98021134146494	37.0	35.0	37.0	35.0	37.0
8	36.002398130655074	37.0	35.0	37.0	35.0	37.0
9	37.76332661976235	39.0	38.0	39.0	35.0	39.0
10	37.1644971046725	39.0	37.0	39.0	33.0	39.0
11	36.84593938839187	39.0	37.0	39.0	32.0	39.0
12	35.947932752486224	37.0	35.0	39.0	32.0	39.0
13	35.564228327130245	37.0	35.0	39.0	31.0	39.0
14	36.55170671178937	38.0	35.0	41.0	31.0	41.0
15	36.83237921253109	38.0	35.0	41.0	32.0	41.0
16	36.99400227299132	38.0	35.0	41.0	32.0	41.0
17	36.944969094423236	38.0	35.0	41.0	32.0	41.0
18	36.8888634627368	38.0	35.0	41.0	32.0	41.0
19	36.8461611826724	38.0	35.0	41.0	32.0	41.0
20	36.69696436281192	38.0	35.0	41.0	32.0	41.0
21	36.55317445864433	38.0	35.0	40.0	31.0	41.0
22	36.43288450662934	38.0	35.0	40.0	31.0	41.0
23	36.3663843083587	38.0	35.0	40.0	31.0	41.0
24	36.254759055559624	37.0	35.0	40.0	31.0	41.0
25	36.105592000299566	37.0	35.0	40.0	31.0	41.0
26	35.94416096951945	37.0	35.0	40.0	30.0	41.0
27	35.903507326092324	37.0	35.0	40.0	30.0	41.0
28	35.97166121995816	37.0	35.0	40.0	30.0	41.0
29	36.00487915412206	37.0	35.0	40.0	30.0	41.0
30	36.015484313255456	37.0	35.0	40.0	30.0	41.0
31	35.8559988631843	37.0	35.0	40.0	30.0	41.0
32	35.625346253516945	37.0	34.0	40.0	30.0	41.0
33	35.51735196191923	37.0	34.0	40.0	29.0	41.0
34	35.364609954114506	37.0	34.0	40.0	29.0	41.0
35	35.29398623851302	36.0	34.0	40.0	29.0	41.0
36	35.09906555157165	36.0	34.0	40.0	27.0	41.0
37	34.8161808689151	36.0	34.0	40.0	26.0	41.0
38	34.92377797912445	36.0	34.0	40.0	27.0	41.0
39	34.843365870628965	36.0	34.0	40.0	27.0	41.0
40	34.67345896981838	36.0	34.0	40.0	25.0	41.0
41	34.67018326352521	36.0	34.0	40.0	26.0	41.0
42	34.6373717921842	36.0	34.0	40.0	26.0	41.0
43	34.549584143725255	35.0	34.0	40.0	26.0	41.0
44	34.38354824121616	35.0	34.0	40.0	25.0	41.0
45	34.326704255409716	35.0	34.0	40.0	25.0	41.0
46	34.16047664326998	35.0	33.0	40.0	24.0	41.0
47	34.18497258936341	35.0	33.0	40.0	24.0	41.0
48	34.04060723625444	35.0	33.0	40.0	24.0	41.0
49	34.03723103442868	35.0	33.0	39.0	24.0	41.0
50	33.89323245800871	35.0	33.0	39.0	24.0	41.0
51	33.18500299406276	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	7.0
13	13.0
14	40.0
15	116.0
16	307.0
17	779.0
18	1723.0
19	3424.0
20	5478.0
21	8685.0
22	13119.0
23	18671.0
24	26885.0
25	36013.0
26	46392.0
27	54906.0
28	60856.0
29	69732.0
30	82351.0
31	99825.0
32	123449.0
33	162488.0
34	288995.0
35	491350.0
36	224057.0
37	268076.0
38	381399.0
39	654843.0
40	534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.764301490438363	25.49677278120106	29.188159321904795	13.550766406455782
2	28.882032006866982	27.51010796228665	29.912431265376377	13.695428765469991
3	28.279538885530147	26.24901704807495	31.336619387892593	14.134824678502309
4	24.73300673352073	29.673674363109566	30.419549645593225	15.173769257776481
5	22.723256106463815	33.430447009889846	29.248840700818718	14.597456182827617
6	21.176329013412314	41.77010398727227	26.100354070725174	10.953212928590244
7	88.16770720082496	4.334525944329956	5.853768758499314	1.6439980963457712
8	88.71598394247815	3.4090709059992315	5.2707666496933765	2.6041785018292427
9	83.60831450108928	5.388960917799455	7.9499647465512275	3.0527598345600295
10	52.555322950715265	23.630788374651186	12.312046949976589	11.501841724656963
11	45.3762933599017	20.77559507597494	19.149711779452634	14.698399784670718
12	39.74588712431393	20.740517654408666	23.29678475105112	16.216810470226278
13	22.008905696464446	38.87800898506873	23.789916969566814	15.323168348900007
14	16.808581934423785	39.96163246991455	26.257658383679782	16.97212721198188
15	15.203885912606651	23.094449478111336	45.57216363361121	16.129500975670798
16	16.51288823200514	18.953169401862752	44.03922270226086	20.494719663871248
17	16.998083223743063	19.676897261240697	27.972835481452012	35.35218403356423
18	21.634927894455366	22.19194198655344	33.48869601285575	22.684434106135445
19	28.051503640402657	24.829981721974946	24.4076444455255	22.71087019209689
20	30.150324034082708	22.959740657516026	24.025441372218488	22.864493936182775
21	22.621096316646703	28.24052485552167	26.523267436214944	22.615111391616686
22	24.342514379022422	25.153679752742587	23.662889336175798	26.840916532059193
23	21.25851771649826	29.683435871848353	22.82285550054616	26.23519091110722
24	21.633071607547663	23.33816714711426	36.00822143070433	19.02053981463375
25	18.937679007667423	25.288836642591477	32.71091179852758	23.062572551213513
26	18.65821181321785	32.57322651789061	25.453054024029953	23.31550764486159
27	19.269762334466414	31.08416436844479	26.08137513734123	23.56469815974757
28	16.473970216836715	28.18576439174439	35.56319264705553	19.77707274436337
29	17.03892153571256	23.683788566360818	34.311415172329035	24.96587472559759
30	19.527626189903913	29.07892643887039	29.72427418381785	21.669173187407846
31	28.14844662391019	26.915103998474006	22.925207320043388	22.01124205757242
32	29.45332030518637	26.79959814588943	23.32597326242744	20.42110828649676
33	26.836851903830254	28.08475678000792	22.729081006760403	22.34931030940142
34	20.021686551873458	28.170466027229168	26.652215366407034	25.15563205449034
35	20.42770130551378	27.015439506330097	28.93445610953629	23.62240307861983
36	29.205794047527988	26.05669932344743	23.854502951976258	20.883003677048325
37	21.529247560502952	30.326287230954417	27.445714009557314	20.698751198985317
38	21.839279479036282	31.118601691077373	24.12561685534116	22.916501974545188
39	20.806511854472227	30.69405607330669	25.447581178146894	23.05185089407419
40	24.178489027264057	25.194390044925342	24.820540262703002	25.8065806651076
41	18.291979208306437	23.456905499313972	26.54016604806439	31.710949244315202
42	22.493268559588568	25.81182947636387	23.136279943428057	28.558622020619506
43	22.87524759826879	25.7079094144791	25.643771501323243	25.773071485928867
44	21.672053632609455	29.0447131508646	26.160619385332197	23.12261383119375
45	18.680711290737094	33.54544078332747	22.736922218698123	25.036925707237312
46	22.481042669955066	30.124432032214898	25.874495161972234	21.520030135857798
47	22.30360724553587	26.504352512724367	26.734692114013143	24.457348127726622
48	23.43805458571677	23.41779545446544	28.923190368303324	24.220959591514465
49	21.4744230868323	22.810085526819023	31.15332705823012	24.562164328118556
50	19.802932741284494	30.008990189523693	27.12816092855312	23.0599161406387
51	19.654365778774768	30.466628922166212	25.087141468585383	24.791863830473638
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2215.0
1	2840.5
2	3466.0
3	11499.5
4	19533.0
5	14219.5
6	8906.0
7	9899.5
8	10893.0
9	12269.0
10	13645.0
11	14542.0
12	15439.0
13	15316.0
14	15193.0
15	14792.0
16	14391.0
17	14233.0
18	14075.0
19	13774.0
20	13473.0
21	13381.5
22	13290.0
23	12728.0
24	12166.0
25	12878.0
26	15139.5
27	16689.0
28	19633.5
29	22578.0
30	28438.0
31	34298.0
32	40899.0
33	47500.0
34	60786.0
35	74072.0
36	75138.0
37	76204.0
38	92010.0
39	107816.0
40	142249.0
41	176682.0
42	219164.0
43	261646.0
44	266367.5
45	271089.0
46	272697.0
47	274305.0
48	267626.5
49	260948.0
50	250820.0
51	240692.0
52	225293.0
53	209894.0
54	188534.0
55	167174.0
56	154966.5
57	142759.0
58	139513.5
59	136268.0
60	126695.5
61	117123.0
62	104749.0
63	92375.0
64	79069.0
65	65763.0
66	58521.5
67	51280.0
68	43453.0
69	35626.0
70	29839.5
71	24053.0
72	21305.5
73	18558.0
74	15244.5
75	9941.0
76	7951.0
77	6322.0
78	4693.0
79	3519.0
80	2345.0
81	1711.0
82	1077.0
83	767.0
84	457.0
85	326.5
86	196.0
87	173.5
88	151.0
89	92.0
90	33.0
91	23.0
92	13.0
93	6.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3124517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.574932644526253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.57959809964436	15.153355398684159
2	7.465076713310586	2.773265942719311
3	2.321930142354777	1.2938908799860573
4	1.159154662220904	0.8612487910136748
5	0.6540275871731833	0.6074259189701952
6	0.4856268965164199	0.5412292135897693
7	0.3436865276158495	0.4468767870906862
8	0.28102601440255176	0.4176031431109651
9	0.23430433140599607	0.39169684567684204
>10	2.6641626952470654	11.964206916798268
>50	1.2717466362155558	17.3856676752712
>100	1.531435586880006	46.14768855005038
>500	0.005774373009032486	0.6832697190374796
>1k	0.0020997720032845404	0.7169099130819944
>5k	1.749810002737117E-4	0.22673815812032427
>10k+	1.749810002737117E-4	0.38892614679870996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11966	0.38297119202743973	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6976	0.22326650807148754	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4110	0.1315403308735398	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.200494668455957E-5	0.0	0.0	0.052872171922892404	0.0
2	3.200494668455957E-5	0.0	0.0	0.20028695635197377	0.0
3	3.200494668455957E-5	0.0	0.0	0.3043350380234769	0.0
4	3.200494668455957E-5	0.0	0.0	0.5804417130711723	0.0
5	3.200494668455957E-5	0.0	0.0	0.9811756505085426	0.0
6	3.200494668455957E-5	0.0	0.0	1.7168733599465134	0.0
7	3.200494668455957E-5	0.0	0.0	2.046588320690846	0.0
8	3.200494668455957E-5	0.0	0.0	2.8890545322685073	0.0
9	3.200494668455957E-5	0.0	0.0	3.2614960968367273	0.0
10	6.400989336911914E-5	0.0	0.0	3.8052921459540787	0.0
11	6.400989336911914E-5	0.0	0.0	4.252209221457268	0.0
12	6.400989336911914E-5	0.0	0.0	4.623786652465005	0.0
13	6.400989336911914E-5	0.0	0.0	4.815048213851933	0.0
14	6.400989336911914E-5	0.0	0.0	4.895220605296755	0.0
15	6.400989336911914E-5	0.0	0.0	4.9811858920914815	0.0
16	6.400989336911914E-5	0.0	0.0	5.126744389612859	0.0
17	6.400989336911914E-5	0.0	0.0	5.286641103248918	0.0
18	6.400989336911914E-5	0.0	0.0	5.5413044640179585	0.0
19	6.400989336911914E-5	0.0	0.0	5.642984179634804	0.0
20	1.2801978673823827E-4	0.0	0.0	5.75266513192279	0.0
21	1.2801978673823827E-4	0.0	0.0	5.906000831488515	0.0
22	2.2403462679191697E-4	0.0	0.0	6.063305144443125	0.0
23	2.2403462679191697E-4	0.0	0.0	6.241604702422807	0.0
24	2.5603957347647654E-4	0.0	0.0	6.3902676797725855	0.0
25	2.5603957347647654E-4	0.0	0.0	6.509710140799362	0.0
26	2.5603957347647654E-4	0.0	0.0	6.626880250611535	0.0
27	2.5603957347647654E-4	0.0	0.0	6.739473653047815	0.0
28	2.5603957347647654E-4	0.0	0.0	6.851490966443774	0.0
29	2.5603957347647654E-4	0.0	0.0	6.970069293910067	0.0
30	2.5603957347647654E-4	0.0	0.0	7.150513183317614	0.0
31	3.840593602147148E-4	0.0	0.0	7.284133835725649	0.0
32	3.840593602147148E-4	0.0	0.0	7.398743549803058	0.0
33	3.840593602147148E-4	0.0	0.0	7.519850268057431	0.0
34	4.1606430689927434E-4	0.0	0.0	7.650526465370488	0.0
35	4.1606430689927434E-4	0.0	0.0	7.81746426727715	0.0
36	4.1606430689927434E-4	0.0	0.0	7.9621906361847286	0.0
37	4.1606430689927434E-4	0.0	0.0	8.106628960572147	0.0
38	4.1606430689927434E-4	0.0	0.0	8.248698918904905	0.0
39	4.1606430689927434E-4	0.0	0.0	8.385104001674499	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATA	25	3.8923507E-5	45.0	8
ACGTTAC	20	7.035151E-4	45.0	35
CGTAGAT	25	3.8923507E-5	45.0	21
ACGTACT	20	7.035151E-4	45.0	19
TATACGA	20	7.035151E-4	45.0	12
TATCCGT	40	6.8212103E-9	45.0	20
GTCGAAT	30	2.1667056E-6	44.999996	38
CCGCGTA	30	2.1667056E-6	44.999996	19
TATAGCG	255	0.0	43.235294	1
TACGTAG	115	0.0	43.043476	1
AACGTTC	95	0.0	42.63158	40
CGTTAGG	440	0.0	41.931816	2
CGGTCTA	335	0.0	40.97015	31
CGTTTTT	8985	0.0	40.96828	1
ATACGTT	50	1.0822987E-9	40.5	12
ATAGGGC	1395	0.0	40.322582	4
TAGCATA	985	0.0	40.203045	30
CGTTTCA	45	1.9301297E-8	40.0	33
ACGAACT	45	1.9301297E-8	40.0	20
CGAATAT	510	0.0	39.705883	14
>>END_MODULE