FastQCFastQC Report
Thu 26 May 2016
SRR1547693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547693_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences719442
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35070.4874611156979992No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.36194717572785573No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG600.045.0000047
GTTACAC302.1643646E-645.00000433
CATACTA302.1643646E-645.00000436
TAAATCG302.1643646E-645.0000041
TTACCTT302.1643646E-645.00000433
TGCCGAC302.1643646E-645.00000443
CTTAAAT207.031525E-445.019
AGCGTGA207.031525E-445.024
CTATGCG253.889346E-545.01
TTGGAAC253.889346E-545.026
ACGTTGC207.031525E-445.039
GTTTACG406.8084773E-945.01
CTGAGTC207.031525E-445.038
CCGTCAA207.031525E-445.031
TCCAGTA351.2110831E-745.031
CTAACCG253.889346E-545.031
TACGAAG207.031525E-445.01
CGCTAAC253.889346E-545.029
TGTTAAT253.889346E-545.045
ACTAACT253.889346E-545.010