FastQCFastQC Report
Thu 26 May 2016
SRR1547691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547691_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1312090
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48900.37268784915668896No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21210.16165049653606078No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA253.891108E-545.00000410
CCAATCG207.0336525E-445.019
ACGTACA207.0336525E-445.034
ACACGAT302.165738E-644.99999616
TACGGGA2500.042.3000034
TACGTAG650.041.538461
AGTACGG1200.041.2499962
TATTGCG556.184564E-1140.9090921
GCGATAC556.184564E-1140.9090929
ATTGCGG1850.040.1351362
TCGATAG451.9286745E-840.01
TTACGGG4150.039.5783163
CGTTTTT31500.039.571431
ACGTAAG800.039.3751
AGGGCGA17650.038.753546
TCACGAC356.2489653E-638.5714325
TACCCGA356.2489653E-638.5714341
ACGTAGG2100.038.571432
ATAGGGC10000.038.0254
TAGCATA4750.037.8947430
Produced by FastQC (version 0.11.5)