##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547691_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1312090
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558693382313713	31.0	31.0	33.0	30.0	34.0
2	31.981208606116958	31.0	31.0	34.0	30.0	34.0
3	32.13405863927017	33.0	31.0	34.0	30.0	34.0
4	35.80440747204841	37.0	35.0	37.0	35.0	37.0
5	35.702259753523	37.0	35.0	37.0	33.0	37.0
6	35.76077022155492	37.0	35.0	37.0	33.0	37.0
7	36.08604440244191	37.0	35.0	37.0	35.0	37.0
8	36.09588061794542	37.0	35.0	37.0	35.0	37.0
9	37.88347902963974	39.0	38.0	39.0	35.0	39.0
10	37.313596628280074	39.0	37.0	39.0	34.0	39.0
11	37.047940308972706	39.0	37.0	39.0	33.0	39.0
12	36.3658361850178	38.0	35.0	39.0	32.0	39.0
13	36.080410642562626	38.0	35.0	39.0	32.0	39.0
14	37.138466873461425	39.0	35.0	41.0	32.0	41.0
15	37.33677186778346	39.0	35.0	41.0	33.0	41.0
16	37.437819814189574	39.0	35.0	41.0	33.0	41.0
17	37.38481887675388	39.0	35.0	41.0	33.0	41.0
18	37.29219413302441	39.0	35.0	41.0	32.0	41.0
19	37.26956153922368	39.0	35.0	41.0	32.0	41.0
20	37.1671920371316	39.0	35.0	41.0	32.0	41.0
21	37.05855238588816	39.0	35.0	41.0	32.0	41.0
22	36.94306716764856	38.0	35.0	41.0	32.0	41.0
23	36.86667530428553	38.0	35.0	41.0	32.0	41.0
24	36.76345601292594	38.0	35.0	41.0	32.0	41.0
25	36.63026774077998	38.0	35.0	40.0	31.0	41.0
26	36.48833311739286	38.0	35.0	40.0	31.0	41.0
27	36.44525527974453	38.0	35.0	40.0	31.0	41.0
28	36.48154699753828	38.0	35.0	40.0	31.0	41.0
29	36.51075002476964	38.0	35.0	40.0	31.0	41.0
30	36.528991913664456	38.0	35.0	40.0	31.0	41.0
31	36.41535565395667	38.0	35.0	40.0	31.0	41.0
32	36.2373739606277	38.0	35.0	41.0	31.0	41.0
33	36.154400231691426	38.0	35.0	41.0	30.0	41.0
34	36.0048106456112	38.0	35.0	40.0	30.0	41.0
35	35.90628691629385	38.0	35.0	40.0	30.0	41.0
36	35.75888315588107	38.0	35.0	40.0	30.0	41.0
37	35.510777461911914	38.0	34.0	40.0	29.0	41.0
38	35.58296001036514	37.0	34.0	40.0	29.0	41.0
39	35.48036110327798	37.0	34.0	40.0	29.0	41.0
40	35.329247993658974	37.0	34.0	40.0	28.0	41.0
41	35.29794602504401	37.0	34.0	40.0	28.0	41.0
42	35.25138443247033	37.0	34.0	40.0	28.0	41.0
43	35.15748691019671	37.0	34.0	40.0	28.0	41.0
44	35.02566897087852	36.0	34.0	40.0	27.0	41.0
45	34.95813549375424	36.0	34.0	40.0	27.0	41.0
46	34.79603457079926	36.0	34.0	40.0	27.0	41.0
47	34.81996814242925	36.0	34.0	40.0	27.0	41.0
48	34.67226943273708	35.0	34.0	40.0	27.0	41.0
49	34.65292243672309	36.0	34.0	40.0	27.0	41.0
50	34.50630520772203	35.0	34.0	40.0	26.0	41.0
51	33.80735772698519	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	4.0
14	8.0
15	37.0
16	100.0
17	186.0
18	457.0
19	890.0
20	1583.0
21	2580.0
22	3949.0
23	5816.0
24	8610.0
25	11863.0
26	15401.0
27	18289.0
28	20957.0
29	24638.0
30	30080.0
31	36842.0
32	46961.0
33	63075.0
34	112406.0
35	184191.0
36	99133.0
37	125444.0
38	183361.0
39	315052.0
40	173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.784793726040135	23.900189773567362	30.073470569854198	13.241545930538301
2	30.075375926956234	25.995930157230067	29.995427142955133	13.933266772858568
3	28.97286009343871	25.91392358755878	31.390072327355593	13.723143991646914
4	25.37211624202608	29.29052122948883	30.457133275918576	14.880229252566515
5	23.454336211692794	32.913976937557635	29.384188584624532	14.247498266125035
6	21.4150706125342	42.65431487169325	25.294758743683744	10.635855772088805
7	89.70421236348116	3.776341561935538	5.0728227484395125	1.446623326143786
8	90.69728448505819	2.9772347933449685	4.417303691057778	1.9081770305390637
9	85.58429680890792	4.467452689983157	6.989078493091175	2.9591720080177426
10	48.80831345410757	21.352193828167277	14.3768339062107	15.462658811514455
11	42.3016713792499	23.11503021896364	19.31079422905441	15.272504172732054
12	37.319010128878354	21.934013672842564	22.629164157946484	18.1178120403326
13	23.349312928229008	33.739530062724356	24.844789610468794	18.066367398577842
14	19.657188150203112	35.59595759437234	26.35718586377459	18.389668391649963
15	18.371986677743145	22.97593915051559	40.968759764955145	17.683314406786117
16	20.4957739179477	20.39936284858508	39.310336943349924	19.794526290117293
17	21.55606703808428	21.24816133039654	26.284858508181603	30.910913123337576
18	24.29398897941452	24.037375484913383	29.85732686020014	21.811308675471956
19	28.739339527014153	24.997751678619608	24.502816117796797	21.760092676569442
20	30.269036422806362	24.623691972349455	23.44496185475082	21.66230975009336
21	25.734438948547737	27.097378990770448	25.865908588587672	21.30227347209414
22	25.51722823891654	24.724066184484297	25.03890739202341	24.719798184575755
23	23.576507709074836	29.242277587665484	23.529178638660458	23.652036064599226
24	24.021065628120024	25.39040767020555	31.174995617678665	19.413531083995764
25	21.698740177884137	25.427905097973465	30.327264135844338	22.54609058829806
26	21.080489905418073	30.317127636061553	26.150568939630663	22.45181351888971
27	21.641350821971052	29.221471088111333	27.20606055987013	21.93111753004748
28	19.411625726893732	27.75846169089011	32.647303157557786	20.182609424658366
29	20.28930942237194	25.018558178173755	32.287800379547136	22.404332019907173
30	22.15236759673498	27.39057534163053	29.870511931346172	20.586545130288318
31	27.955932900944298	25.842587017658854	24.51226668902286	21.689213392374
32	28.96958287922322	25.71096494905075	25.286146529582577	20.03330564214345
33	27.541632052679315	26.526762645855083	24.83556768209498	21.096037619370623
34	22.475135089818536	26.324261293051542	28.343634964064968	22.856968653064957
35	22.60050758713198	26.278837579739196	29.11256087615941	22.008093956969415
36	29.286634301000692	24.632837686439192	25.79632494722161	20.284203065338506
37	23.78868827595668	29.007232735559295	26.865839995732	20.33823899275202
38	23.950110129640496	29.996646571500428	23.897598487908603	22.155644810950466
39	22.794930225822924	29.59735993719943	25.367772027833457	22.239937809144187
40	24.809654825507398	25.97512365767592	25.48697116813633	23.728250348680348
41	20.729142055804097	24.88182975253222	26.20460486704418	28.1844233246195
42	23.796462132932955	26.919799708861436	24.032345342164028	25.251392816041584
43	23.02090557812345	26.656403143077075	26.169546296366864	24.153144982432607
44	22.789519011653166	28.52662546014374	26.449786218933152	22.234069309269945
45	20.849331981800027	30.98796576454359	24.151544482466907	24.011157771189477
46	23.480706353984864	28.361392892255864	26.824989139464517	21.33291161429475
47	23.404110998483336	26.003399157070017	27.304148343482538	23.288341500964112
48	25.18676310314079	23.430328712207242	28.290132536640016	23.09277564801195
49	22.908489509103795	23.696850063638927	29.2490606589487	24.145599768308575
50	21.905509530596223	28.954644879543324	26.50748043198256	22.63236515787789
51	21.288402472391375	30.596071915798458	24.24574533759117	23.869780274218993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	953.0
1	1292.0
2	1631.0
3	4973.5
4	8316.0
5	5914.0
6	3512.0
7	3796.0
8	4080.0
9	4473.5
10	4867.0
11	5210.5
12	5554.0
13	5832.0
14	6110.0
15	6064.5
16	6019.0
17	5554.0
18	5089.0
19	4784.0
20	4479.0
21	4476.5
22	4474.0
23	4612.0
24	4750.0
25	5638.5
26	6607.0
27	6687.0
28	8305.0
29	9923.0
30	12724.0
31	15525.0
32	17354.0
33	19183.0
34	21734.0
35	24285.0
36	26317.5
37	28350.0
38	36166.0
39	43982.0
40	58045.0
41	72108.0
42	86860.0
43	101612.0
44	106095.5
45	110579.0
46	111465.5
47	112352.0
48	107198.0
49	102044.0
50	97703.5
51	93363.0
52	91561.0
53	89759.0
54	83667.5
55	77576.0
56	70994.5
57	64413.0
58	63953.5
59	63494.0
60	58945.5
61	54397.0
62	49647.5
63	44898.0
64	40200.0
65	35502.0
66	30094.0
67	24686.0
68	21230.0
69	17774.0
70	14943.0
71	12112.0
72	10810.5
73	9509.0
74	7305.5
75	4291.5
76	3481.0
77	2403.0
78	1325.0
79	1146.0
80	967.0
81	701.0
82	435.0
83	286.5
84	138.0
85	114.0
86	90.0
87	69.5
88	49.0
89	31.5
90	14.0
91	10.5
92	7.0
93	6.0
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1312090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.063088993414706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92306952210373	16.413005479841118
2	5.618874013245822	2.36701686767564
3	2.072614852169785	1.3096701324097575
4	1.2140993130511595	1.0229072751056092
5	0.9190913978010248	0.9679451952482451
6	0.7236003849678004	0.9144755582547552
7	0.5969038660380046	0.8800847475610246
8	0.48498307705678084	0.817219336987766
9	0.432298593839166	0.8194989378386154
>10	7.856126834592722	42.1650847287794
>50	2.034077930333986	28.333046388404064
>100	0.12207381805866739	3.2672549877738355
>500	0.0014575978275661022	0.184669599243176
>1k	7.287989137830511E-4	0.5381207648769356
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4890	0.37268784915668896	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2121	0.16165049653606078	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.621428408112249E-5	0.0	0.0	0.028808999382664298	0.0
2	7.621428408112249E-5	0.0	0.0	0.09214306945407709	0.0
3	7.621428408112249E-5	0.0	0.0	0.13954835415253528	0.0
4	7.621428408112249E-5	0.0	0.0	0.2624057800913047	0.0
5	7.621428408112249E-5	0.0	0.0	0.4200931338551471	0.0
6	7.621428408112249E-5	0.0	0.0	0.8660991242978759	0.0
7	7.621428408112249E-5	0.0	0.0	1.0667713342834715	0.0
8	7.621428408112249E-5	0.0	0.0	1.6623097500933626	0.0
9	7.621428408112249E-5	0.0	0.0	1.9878971716879177	0.0
10	7.621428408112249E-5	0.0	0.0	2.4486125189583032	0.0
11	7.621428408112249E-5	0.0	0.0	2.7707702977692077	0.0
12	7.621428408112249E-5	0.0	0.0	3.0398067205755703	0.0
13	7.621428408112249E-5	0.0	0.0	3.197570288623494	0.0
14	7.621428408112249E-5	0.0	0.0	3.272870001295643	0.0
15	7.621428408112249E-5	0.0	0.0	3.3349084285376764	0.0
16	7.621428408112249E-5	0.0	0.0	3.431776783604783	0.0
17	7.621428408112249E-5	0.0	0.0	3.5281116386833222	0.0
18	7.621428408112249E-5	0.0	0.0	3.6784824211753766	0.0
19	7.621428408112249E-5	0.0	0.0	3.7566782766426083	0.0
20	2.2864285224336744E-4	0.0	0.0	3.8376940606208416	0.0
21	2.2864285224336744E-4	0.0018291428179469395	0.0	3.935095915676516	0.0
22	2.2864285224336744E-4	0.0018291428179469395	0.0	4.041872127674169	0.0
23	2.2864285224336744E-4	0.0018291428179469395	0.0	4.16213826795418	0.0
24	2.2864285224336744E-4	0.0018291428179469395	0.0	4.2583969087486375	0.0
25	2.2864285224336744E-4	0.0018291428179469395	0.0	4.3468816925668206	0.0
26	2.2864285224336744E-4	0.0018291428179469395	0.0	4.438415047748249	0.0
27	2.2864285224336744E-4	0.0018291428179469395	0.0	4.52811926011173	0.0
28	2.2864285224336744E-4	0.0018291428179469395	0.0	4.616222972509508	0.0
29	3.0485713632448996E-4	0.0018291428179469395	0.0	4.721246255973295	0.0
30	3.0485713632448996E-4	0.0018291428179469395	0.0	4.865062610034372	0.0
31	3.0485713632448996E-4	0.0018291428179469395	0.0	4.95857753660191	0.0
32	3.810714204056124E-4	0.0018291428179469395	0.0	5.0581133916118555	0.0
33	3.810714204056124E-4	0.0018291428179469395	0.0	5.148427318247986	0.0
34	3.810714204056124E-4	0.0018291428179469395	0.0	5.240570387702063	0.0
35	3.810714204056124E-4	0.0018291428179469395	0.0	5.355044242391909	0.0
36	3.810714204056124E-4	0.0018291428179469395	0.0	5.457247597344694	0.0
37	3.810714204056124E-4	0.0018291428179469395	0.0	5.56082280941094	0.0
38	3.810714204056124E-4	0.0018291428179469395	0.0	5.673162664146514	0.0
39	3.810714204056124E-4	0.0018291428179469395	0.0	5.812863446867212	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGATA	25	3.891108E-5	45.000004	10
CCAATCG	20	7.0336525E-4	45.0	19
ACGTACA	20	7.0336525E-4	45.0	34
ACACGAT	30	2.165738E-6	44.999996	16
TACGGGA	250	0.0	42.300003	4
TACGTAG	65	0.0	41.53846	1
AGTACGG	120	0.0	41.249996	2
TATTGCG	55	6.184564E-11	40.909092	1
GCGATAC	55	6.184564E-11	40.909092	9
ATTGCGG	185	0.0	40.135136	2
TCGATAG	45	1.9286745E-8	40.0	1
TTACGGG	415	0.0	39.578316	3
CGTTTTT	3150	0.0	39.57143	1
ACGTAAG	80	0.0	39.375	1
AGGGCGA	1765	0.0	38.75354	6
TCACGAC	35	6.2489653E-6	38.57143	25
TACCCGA	35	6.2489653E-6	38.57143	41
ACGTAGG	210	0.0	38.57143	2
ATAGGGC	1000	0.0	38.025	4
TAGCATA	475	0.0	37.89474	30
>>END_MODULE