FastQCFastQC Report
Thu 26 May 2016
SRR1547690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547690_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3522442
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143480.4073310504473885No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC117110.3324682138130309No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT42200.11980325013158485No Hit
GAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT38490.10927078430248106TruSeq Adapter, Index 23 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAT600.045.00000415
GTACGAT302.1667838E-645.00000439
CGTATAA253.8924532E-545.026
CGTCGAA253.8924532E-545.025
GCTCGAC253.8924532E-545.013
TCGACCC253.8924532E-545.015
ACGCGTA253.8924532E-545.023
TCGATAT1100.042.95454416
TTACGAT800.042.187513
AACTCCG750.042.01
CATTACG700.041.78571720
CATACGA4700.041.64893718
TACGAAT2600.041.53846412
GCGATAA3050.041.3114789
GGACTAA10950.041.301378
GCTACGA2650.040.7547210
ACGGGAT6300.040.7142875
CGCCTAG501.0822987E-940.545
AATACGG1000.040.52
TACGGGA6250.040.324
Produced by FastQC (version 0.11.5)