FastQCFastQC Report
Thu 26 May 2016
SRR1547689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547689_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2312419
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258941.119779763096567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT156500.042.541531
AGGGCGA29350.038.177176
GGGCGAT60100.037.2878577
ATACCGG1100.036.818182
GTTTTTT187600.036.772392
CGTTAGG3800.036.7105262
ACGGGAT4800.036.5625045
GTAGGGA19900.036.2939724
TTAACGC250.002107548336.00000428
AATACGG2000.036.0000042
TAAGGGA16700.035.838324
TACGGGA5350.035.747664
ATAGGGC11400.035.7236864
CGGGTAT2400.035.6250046
AGGGATC13150.035.5893556
TAACGGG6100.035.4098363
TAGAACG702.1827873E-1135.3571431
TAGGGAC16000.035.296885
TAGGGCG13550.035.2029535
AGGGTAC10450.035.0956956
Produced by FastQC (version 0.11.5)