##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547689_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2312419
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42349202285572	31.0	31.0	33.0	30.0	34.0
2	31.836824554719538	31.0	31.0	34.0	30.0	34.0
3	31.94562706845083	31.0	31.0	34.0	30.0	34.0
4	35.67043559147369	37.0	35.0	37.0	33.0	37.0
5	35.56332438022694	37.0	35.0	37.0	33.0	37.0
6	35.60701758634573	37.0	35.0	37.0	33.0	37.0
7	35.99116596084014	37.0	35.0	37.0	35.0	37.0
8	35.99521669731999	37.0	35.0	37.0	35.0	37.0
9	37.73535721683657	39.0	37.0	39.0	35.0	39.0
10	37.1408896917038	39.0	37.0	39.0	33.0	39.0
11	36.89627485330297	39.0	37.0	39.0	32.0	39.0
12	36.488816689362956	39.0	35.0	39.0	32.0	39.0
13	36.35848261063415	39.0	35.0	39.0	32.0	39.0
14	37.43628944408431	40.0	36.0	41.0	32.0	41.0
15	37.54044357878049	40.0	36.0	41.0	32.0	41.0
16	37.57554145680346	40.0	35.0	41.0	32.0	41.0
17	37.47576498895745	39.0	35.0	41.0	32.0	41.0
18	37.31726949138543	39.0	36.0	41.0	32.0	41.0
19	37.22795566028475	39.0	35.0	41.0	32.0	41.0
20	37.078953251984174	39.0	35.0	41.0	32.0	41.0
21	36.95428380410298	39.0	35.0	41.0	31.0	41.0
22	36.869825926875706	38.0	35.0	41.0	31.0	41.0
23	36.75094089782172	38.0	35.0	41.0	31.0	41.0
24	36.60396796601308	38.0	35.0	40.0	31.0	41.0
25	36.4715110021151	38.0	35.0	40.0	31.0	41.0
26	36.30740882167116	38.0	35.0	40.0	30.0	41.0
27	36.286618039377814	38.0	35.0	40.0	30.0	41.0
28	36.27767761811332	38.0	35.0	40.0	30.0	41.0
29	36.22018760440906	38.0	35.0	40.0	30.0	41.0
30	36.1962010345011	38.0	35.0	40.0	30.0	41.0
31	36.05069539733067	38.0	35.0	40.0	30.0	41.0
32	35.77872824950842	38.0	35.0	40.0	29.0	41.0
33	35.547795187636844	38.0	35.0	40.0	27.0	41.0
34	35.218286997295905	38.0	34.0	40.0	25.0	41.0
35	35.042587437657275	38.0	34.0	40.0	24.0	41.0
36	34.88697333830936	38.0	34.0	40.0	24.0	41.0
37	34.646520375416394	38.0	34.0	40.0	23.0	41.0
38	34.68186950548322	37.0	34.0	40.0	23.0	41.0
39	34.540567691235886	37.0	34.0	40.0	23.0	41.0
40	34.38120773095187	37.0	33.0	40.0	23.0	41.0
41	34.28563768071444	37.0	33.0	40.0	23.0	41.0
42	34.244803817993194	37.0	33.0	40.0	23.0	41.0
43	34.144699554881704	37.0	33.0	40.0	22.0	41.0
44	33.96861295465917	36.0	33.0	40.0	22.0	41.0
45	33.89436429989548	36.0	33.0	40.0	22.0	41.0
46	33.6926370177723	36.0	33.0	40.0	21.0	41.0
47	33.70537043675908	35.0	33.0	40.0	22.0	41.0
48	33.52936383933881	35.0	33.0	40.0	20.0	41.0
49	33.48444550922648	35.0	33.0	40.0	20.0	41.0
50	33.341605911385436	35.0	33.0	39.0	20.0	41.0
51	32.65444973423934	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	9.0
14	41.0
15	99.0
16	268.0
17	638.0
18	1408.0
19	2657.0
20	4589.0
21	7259.0
22	10449.0
23	15144.0
24	21635.0
25	32071.0
26	44406.0
27	51663.0
28	53417.0
29	55658.0
30	61586.0
31	71810.0
32	85760.0
33	108191.0
34	170119.0
35	233100.0
36	191968.0
37	238061.0
38	331677.0
39	518442.0
40	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.29970952496066	23.533883781442725	24.85427597680178	17.312130716794837
2	35.221471541273445	24.80246875674348	25.836104961946777	14.1399547400363
3	28.25132469504878	25.26566335945172	32.348895247790296	14.134116697709196
4	24.993826810798563	27.898101511879986	32.04081959195111	15.067252085370342
5	23.079467864604123	30.657117070911454	31.14755587114619	15.115859193338233
6	21.337006831374417	39.85670416996228	28.499419871571718	10.306869127091586
7	84.91164447273613	3.6638688749746477	9.747108979817238	1.677377672471987
8	84.8967682759915	3.28629889306393	9.634542874799074	2.182389956145491
9	79.6119128929489	4.972930943743327	11.873972666718272	3.541183496589502
10	42.0610192184029	25.425279761150556	18.719834078512587	13.793866941933967
11	33.52052547570315	25.784081518098578	24.74188285081553	15.95351015538274
12	29.347752288836926	22.661031586403674	29.377288458536277	18.61392766622312
13	24.192847403519863	26.242129994607378	31.121176568779273	18.443846033093482
14	21.32113600519629	27.783848861300655	31.872251525350727	19.022763608152328
15	21.20251563406113	22.90514824519259	36.992128156705164	18.90020796404112
16	23.92455692502094	21.830256540877755	35.39259104859457	18.852595485506736
17	23.821547911516035	21.952206758377265	30.62918095725731	23.59706437284938
18	25.736512284322178	22.240216846514407	31.47638901081508	20.546881858348335
19	27.945238298076603	24.393243611992464	27.54608918193459	20.115428907996343
20	29.79351925407982	23.65799623684116	26.989096699170865	19.559387809908152
21	26.91125613480948	25.146697030252735	28.531507481991802	19.410539352945985
22	26.574855162494345	22.569049986183302	28.70033501714006	22.1557598341823
23	25.080489305787573	25.626670599056663	27.854467551079626	21.438372544076138
24	24.074183787626723	23.597626554703105	32.19991705655419	20.128272601115974
25	24.230383853445243	24.146272799176966	30.04481454269317	21.57852880468462
26	22.384048911551062	26.120612224687655	28.987826168181456	22.507512695579823
27	22.55590357975782	25.030844323628198	29.69414280024511	22.719109296368867
28	21.472276434331324	26.33428457385967	31.60707466942626	20.586364322382753
29	23.209937299425405	24.634549361512768	30.7894892750838	21.366024063978024
30	24.0462044292146	24.553984377398734	30.751390643304692	20.64842055008197
31	26.54527574803701	24.897823448086182	27.73844186542318	20.818458938453627
32	27.117490385609184	24.75412111732346	27.57225226051161	20.556136236555744
33	25.843499815561106	26.024565617217295	26.41182242491521	21.720112142306387
34	23.036093372351637	26.959992977051307	28.985490951250615	21.01842269934644
35	23.19311508857175	27.908566743310793	28.190392831057004	20.707925337060455
36	25.333817098025918	28.17871674640279	25.33822806333973	21.149238092231553
37	23.94401706611129	28.736141676746296	26.217523727317584	21.10231752982483
38	24.15578664593225	29.080845642593317	25.526386005304403	21.236981706170035
39	23.94406031086927	27.994926524993957	25.664639496561826	22.396373667574952
40	25.05510463285417	26.148332114551902	26.698664904586927	22.097898348007
41	21.52248359834442	26.235254078088786	27.524207334397445	24.71805498916935
42	24.20819929260225	26.279579955016803	25.853056907074368	23.659163845306583
43	23.415739102645325	25.685656448939397	27.264436073220295	23.63416837519498
44	23.338503964895636	26.7513370198048	27.108711699739533	22.801447315560026
45	22.85891959891352	27.19684451649982	26.669820651015236	23.274415233571425
46	23.421274431666582	27.308372747326505	26.624586634169674	22.645766186837246
47	22.800928378464285	26.96068489317896	28.133871932379034	22.10451479597772
48	23.632135871569986	25.674196588075088	28.497646836494596	22.19602070386033
49	23.7063006315032	25.407722389411262	28.16388379441615	22.722093184669387
50	22.18732850750664	26.953117060532715	28.474770359523944	22.3847840724367
51	22.40303336030365	27.294793893321234	27.082116173582726	23.220056572792387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1549.0
1	2311.5
2	3074.0
3	22483.0
4	41892.0
5	28807.0
6	15722.0
7	15882.5
8	16043.0
9	16667.5
10	17292.0
11	17294.5
12	17297.0
13	17160.0
14	17023.0
15	16435.5
16	15848.0
17	15258.5
18	14669.0
19	14116.5
20	13564.0
21	13318.0
22	13072.0
23	13072.0
24	13072.0
25	14602.0
26	16854.5
27	17577.0
28	20728.5
29	23880.0
30	27193.0
31	30506.0
32	34779.5
33	39053.0
34	44306.5
35	49560.0
36	54371.5
37	59183.0
38	66467.5
39	73752.0
40	83873.5
41	93995.0
42	107749.5
43	121504.0
44	131794.5
45	142085.0
46	146511.5
47	150938.0
48	150605.0
49	150272.0
50	148627.0
51	146982.0
52	145199.0
53	143416.0
54	138085.0
55	132754.0
56	130182.0
57	127610.0
58	125825.5
59	124041.0
60	118481.0
61	112921.0
62	105296.5
63	97672.0
64	87592.0
65	77512.0
66	66948.5
67	56385.0
68	49024.5
69	41664.0
70	35575.0
71	29486.0
72	24953.0
73	20420.0
74	17512.5
75	12148.0
76	9691.0
77	7232.0
78	4773.0
79	3397.0
80	2021.0
81	1596.0
82	1171.0
83	783.5
84	396.0
85	293.0
86	190.0
87	146.5
88	103.0
89	69.0
90	35.0
91	23.5
92	12.0
93	7.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2312419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.625965477902785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30949363825162	19.555044496100784
2	5.606260883982992	2.8733169574612987
3	2.359571497832409	1.8139889323829002
4	1.5031133755280228	1.5407492588262015
5	1.1105980250087921	1.4230073324351162
6	0.937736456802483	1.4418241241634782
7	0.7851454959540378	1.4084077962114325
8	0.6722955657169449	1.3782578366407643
9	0.6495267348143919	1.4980274714995674
>10	9.469223949529109	54.89346397994329
>50	0.5555203022029707	8.834119154258566
>100	0.039122512475384605	1.7756405020709272
>500	0.0018790843505962812	0.32499134144115965
>1k	3.416517001084148E-4	0.10563094112304931
>5k	0.0	0.0
>10k+	1.708258500542074E-4	1.1335298754414584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25894	1.119779763096567	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021665623747253417	0.0
2	0.0	0.0	0.0	0.07546210267256928	0.0
3	0.0	0.0	0.0	0.13470742110318243	0.0
4	0.0	0.0	0.0	0.21068846087149432	0.0
5	0.0	0.0	0.0	0.3398605529534224	0.0
6	0.0	0.0	0.0	0.717344045348183	0.0
7	0.0	0.0	0.0	0.9129400856851635	0.0
8	0.0	0.0	0.0	1.4290662721591545	0.0
9	0.0	0.0	0.0	1.7493369497482938	0.0
10	8.648951595709946E-5	0.0	0.0	2.158951297321117	0.0
11	8.648951595709946E-5	0.0	0.0	2.412668292381268	0.0
12	8.648951595709946E-5	0.0	0.0	2.611594179082597	0.0
13	8.648951595709946E-5	0.0	0.0	2.7435339356751522	0.0
14	8.648951595709946E-5	0.0	0.0	2.820769073424842	0.0
15	8.648951595709946E-5	0.0	0.0	2.87837109105227	0.0
16	8.648951595709946E-5	0.0	0.0	2.9614442711290643	0.0
17	8.648951595709946E-5	0.0	0.0	3.043998514110116	0.0
18	8.648951595709946E-5	0.0	0.0	3.172305711032473	0.0
19	8.648951595709946E-5	0.0	0.0	3.238945883077418	0.0
20	1.7297903191419893E-4	0.0	0.0	3.313975538170202	0.0
21	1.7297903191419893E-4	0.0	0.0	3.3948864803480685	0.0
22	2.1622378989274868E-4	0.0	0.0	3.4843166398477092	0.0
23	2.1622378989274868E-4	0.0	0.0	3.582525485216996	0.0
24	2.1622378989274868E-4	0.0	0.0	3.66797712698261	0.0
25	2.1622378989274868E-4	0.0	0.0	3.747677215937077	0.0
26	2.594685478712984E-4	0.0	0.0	3.825777248846338	0.0
27	2.594685478712984E-4	0.0	0.0	3.90742335190984	0.0
28	2.594685478712984E-4	0.0	0.0	3.992010098515883	0.0
29	2.594685478712984E-4	0.0	0.0	4.080834831403824	0.0
30	2.594685478712984E-4	0.0	0.0	4.198893020685265	0.0
31	3.0271330584984813E-4	0.0	0.0	4.298399208793908	0.0
32	3.0271330584984813E-4	0.0	0.0	4.4143384049344	0.0
33	3.0271330584984813E-4	0.0	0.0	4.516828481343563	0.0
34	3.0271330584984813E-4	0.0	0.0	4.626583677093122	0.0
35	3.0271330584984813E-4	0.0	0.0	4.741614733316064	0.0
36	3.0271330584984813E-4	0.0	0.0	4.843499383113527	0.0
37	3.0271330584984813E-4	0.0	0.0	4.956065488131692	0.0
38	3.0271330584984813E-4	0.0	0.0	5.093237860439652	0.0
39	3.0271330584984813E-4	0.0	0.0	5.281568781436237	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15650	0.0	42.54153	1
AGGGCGA	2935	0.0	38.17717	6
GGGCGAT	6010	0.0	37.287857	7
ATACCGG	110	0.0	36.81818	2
GTTTTTT	18760	0.0	36.77239	2
CGTTAGG	380	0.0	36.710526	2
ACGGGAT	480	0.0	36.562504	5
GTAGGGA	1990	0.0	36.293972	4
TTAACGC	25	0.0021075483	36.000004	28
AATACGG	200	0.0	36.000004	2
TAAGGGA	1670	0.0	35.83832	4
TACGGGA	535	0.0	35.74766	4
ATAGGGC	1140	0.0	35.723686	4
CGGGTAT	240	0.0	35.625004	6
AGGGATC	1315	0.0	35.589355	6
TAACGGG	610	0.0	35.409836	3
TAGAACG	70	2.1827873E-11	35.357143	1
TAGGGAC	1600	0.0	35.29688	5
TAGGGCG	1355	0.0	35.202953	5
AGGGTAC	1045	0.0	35.095695	6
>>END_MODULE