FastQCFastQC Report
Thu 26 May 2016
SRR1547688_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547688_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences794237
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.5524799272761154No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8540.10752458019457668No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA207.0319674E-445.00000442
CGTTCGA207.0319674E-445.00000414
TCTAACG207.0319674E-445.0000041
TATAGCG406.8102963E-945.0000041
TATACGA207.0319674E-445.00000438
GCGTTAG351.2113014E-745.01
ATATACG253.889713E-545.01
CGAATAT1100.045.014
TCGTAAG253.889713E-545.01
TACGTAG502.1827873E-1145.01
CACGACG302.164652E-644.99999626
AGTTGCG302.164652E-644.9999961
CGGTCTA302.164652E-644.99999631
ATAGCGG1450.041.896552
CGTTTTT28650.041.7801061
TACGGGA1900.041.447374
TACGGGT1250.041.3999984
ACGTAGG1200.041.2499962
AATGCGG950.040.2631572
CGTAAGG850.039.7058832
Produced by FastQC (version 0.11.5)