##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547688_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	794237
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.382491624036653	31.0	31.0	33.0	30.0	34.0
2	31.791513112584784	31.0	31.0	34.0	30.0	34.0
3	31.937969397043954	31.0	31.0	34.0	30.0	34.0
4	35.64625294465002	37.0	35.0	37.0	33.0	37.0
5	35.52807537296802	37.0	35.0	37.0	33.0	37.0
6	35.5753257528924	37.0	35.0	37.0	33.0	37.0
7	35.975545082890875	37.0	35.0	37.0	35.0	37.0
8	35.9894414387645	37.0	35.0	37.0	35.0	37.0
9	37.74970821052155	39.0	37.0	39.0	35.0	39.0
10	37.090941368886114	39.0	37.0	39.0	33.0	39.0
11	36.825117691570654	39.0	37.0	39.0	32.0	39.0
12	36.244316243136495	38.0	35.0	39.0	32.0	39.0
13	36.00352035979185	38.0	35.0	39.0	31.0	39.0
14	37.00391193056984	39.0	35.0	41.0	31.0	41.0
15	37.16796623677819	39.0	35.0	41.0	32.0	41.0
16	37.243768547675316	39.0	35.0	41.0	32.0	41.0
17	37.14685289151727	39.0	35.0	41.0	32.0	41.0
18	37.06141869492355	39.0	35.0	41.0	32.0	41.0
19	37.03034736482939	39.0	35.0	41.0	32.0	41.0
20	36.94683199095484	38.0	35.0	41.0	32.0	41.0
21	36.822690204561106	38.0	35.0	41.0	31.0	41.0
22	36.74194478474309	38.0	35.0	41.0	31.0	41.0
23	36.64625798093012	38.0	35.0	40.0	31.0	41.0
24	36.51369175699445	38.0	35.0	40.0	31.0	41.0
25	36.37819945431905	38.0	35.0	40.0	31.0	41.0
26	36.21922801380445	38.0	35.0	40.0	30.0	41.0
27	36.21573535355316	38.0	35.0	40.0	30.0	41.0
28	36.23407874475755	38.0	35.0	40.0	30.0	41.0
29	36.221022188591064	38.0	35.0	40.0	30.0	41.0
30	36.23207304620661	38.0	35.0	40.0	30.0	41.0
31	36.11998685530893	38.0	35.0	40.0	30.0	41.0
32	35.93189564323999	38.0	35.0	40.0	30.0	41.0
33	35.81405550232487	38.0	35.0	40.0	29.0	41.0
34	35.59516366021729	38.0	34.0	40.0	28.0	41.0
35	35.50307653760779	38.0	34.0	40.0	28.0	41.0
36	35.34204651760117	38.0	34.0	40.0	27.0	41.0
37	35.095506756799296	37.0	34.0	40.0	26.0	41.0
38	35.15842500412345	37.0	34.0	40.0	27.0	41.0
39	35.02220873618328	37.0	34.0	40.0	26.0	41.0
40	34.86045349184185	37.0	34.0	40.0	25.0	41.0
41	34.792316399261175	37.0	34.0	40.0	25.0	41.0
42	34.75736209720776	37.0	34.0	40.0	25.0	41.0
43	34.68193876638837	36.0	34.0	40.0	25.0	41.0
44	34.54389936505098	36.0	34.0	40.0	25.0	41.0
45	34.47809658829795	36.0	33.0	40.0	24.0	41.0
46	34.302730796978736	36.0	33.0	40.0	24.0	41.0
47	34.30509029420689	35.0	33.0	40.0	24.0	41.0
48	34.141999176568206	35.0	33.0	40.0	23.0	41.0
49	34.100233305675765	35.0	33.0	40.0	24.0	41.0
50	33.95924138512812	35.0	33.0	39.0	24.0	41.0
51	33.26389478203609	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	5.0
14	9.0
15	28.0
16	60.0
17	209.0
18	410.0
19	799.0
20	1377.0
21	2113.0
22	3209.0
23	4622.0
24	6488.0
25	9040.0
26	11712.0
27	13867.0
28	15479.0
29	17630.0
30	20809.0
31	25486.0
32	30895.0
33	39568.0
34	65744.0
35	95186.0
36	63709.0
37	79254.0
38	111033.0
39	175410.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.933872383180336	24.223625945404205	28.83031135542665	14.012190315988803
2	31.105324984859685	25.725444672056323	29.261668746230658	13.907561596853332
3	28.171440010979094	25.995263378563326	31.851575789090663	13.981720821366922
4	24.783408478829365	28.60191605276511	31.157198669918422	15.457476798487102
5	22.884479066072217	32.58939082414947	29.717074374525488	14.809055735252826
6	21.218981236079408	40.944453607676294	26.788477494752826	11.04808766149147
7	88.54611406922619	3.6325429311402013	6.25631895769147	1.5650240419421406
8	89.4196568530552	2.7384773058923217	5.6748804198243095	2.1669854212281727
9	84.45917276581172	4.214107376009932	8.136740041070864	3.1899798171074885
10	45.38695628634778	23.55543748276648	15.729058203030078	15.328548027855666
11	39.158714590229366	23.951415005848382	20.867448884904633	16.022421519017623
12	35.10853813156526	21.934258917678225	24.324729268467724	18.63247368228879
13	24.679283387704174	29.958941726462	26.068918975066634	19.29285591076719
14	21.22741700525158	31.28033571843165	28.324542926103923	19.167704350212848
15	20.86379758183011	22.87856143695144	37.76958263087718	18.488058350341273
16	23.318984131940464	20.777677192072392	36.159987510025346	19.743351165961798
17	23.484677747322273	21.89308732783791	27.803917470477955	26.81831745436186
18	26.04524845858352	22.821651471789906	29.739108099975198	21.393991969651378
19	28.480541702287855	26.70172756998226	24.097844849837013	20.71988587789287
20	31.194844863686782	25.620186417908002	23.028264863006886	20.156703855398327
21	26.72854576152962	27.792585840246677	25.486976809189198	19.99189158903451
22	26.339367216586485	23.304127105637235	26.229954031353365	24.126551646422918
23	25.394309255297852	27.701806891393876	24.150473976911176	22.753409876397097
24	25.256189273478824	24.354191507069046	30.6518079616034	19.73781125784873
25	23.891357365622603	24.502761770101365	28.494643286575673	23.111237577700358
26	21.728778689484372	28.39429540552757	26.248840081738827	23.628085823249233
27	22.053241035106648	26.863643975286973	27.039158336869225	24.043956652737155
28	20.045276158124086	27.96633750379295	30.427945311034364	21.5604410270486
29	22.84620333729101	24.81501113647438	29.39865556502656	22.940129961208054
30	23.48631453835568	26.2843458564635	29.08439168661243	21.144947918568388
31	27.29512727309355	25.635421165218947	24.94381400010324	22.125637561584263
32	28.802611814861308	24.506035352168183	24.155384350011396	22.535968482959117
33	26.201373141770024	26.085035071395566	24.24578557785648	23.467806208977926
34	21.38543029347663	27.175137899644564	28.18277164121037	23.256660165668432
35	22.740567362134982	26.476857663392668	27.867248692770545	22.91532628170181
36	26.89285439988316	28.110878742743033	23.858621544954467	21.13764531241934
37	23.832432888419955	29.124807834437327	25.693086572395895	21.34967270474682
38	24.568233411437642	29.104033179013317	24.216952874268006	22.11078053528103
39	23.900926297817907	27.98748988022467	24.583342251746014	23.52824157021141
40	25.86016516480597	25.032200715907216	25.70869904071455	23.398935078572265
41	21.36188568399609	25.000220337254497	26.401313461850805	27.236580516898606
42	24.165960538227253	25.98317630631663	24.260642604159717	25.5902205512964
43	22.935975030123252	25.645619632427096	26.33659726252995	25.081808074919703
44	23.388232983353834	26.847905599965756	26.242292917605198	23.52156849907521
45	21.830889268568452	28.890746716660143	24.303702799038575	24.974661215732834
46	23.874108106270548	27.68254312000071	25.17220930276479	23.271139470963956
47	23.016807325773037	26.6911513817664	26.543714281757207	23.748327010703356
48	24.314530801259572	25.011300153480637	27.20006748615338	23.474101559106415
49	23.317599154912198	24.79436238805294	27.706213636483824	24.181824820551046
50	22.16907547747083	27.518234481647163	27.025812194596828	23.286877846285176
51	21.33368251542046	28.98656194561573	25.161507207546364	24.518248331417446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	491.0
1	597.0
2	703.0
3	3942.5
4	7182.0
5	4894.5
6	2607.0
7	2776.0
8	2945.0
9	3115.5
10	3286.0
11	3422.0
12	3558.0
13	3557.0
14	3556.0
15	3505.0
16	3454.0
17	3418.5
18	3383.0
19	3326.0
20	3269.0
21	3132.0
22	2995.0
23	3098.5
24	3202.0
25	3295.5
26	4170.5
27	4952.0
28	5473.5
29	5995.0
30	6934.5
31	7874.0
32	9233.0
33	10592.0
34	13474.5
35	16357.0
36	17671.5
37	18986.0
38	21563.0
39	24140.0
40	30017.5
41	35895.0
42	41654.5
43	47414.0
44	49751.0
45	52088.0
46	52781.0
47	53474.0
48	56497.0
49	59520.0
50	62160.0
51	64800.0
52	62826.0
53	60852.0
54	57267.0
55	53682.0
56	50667.0
57	47652.0
58	45529.5
59	43407.0
60	41506.5
61	39606.0
62	35721.5
63	31837.0
64	28017.5
65	24198.0
66	19732.5
67	15267.0
68	12929.0
69	10591.0
70	8881.0
71	7171.0
72	6209.0
73	5247.0
74	4501.0
75	3108.5
76	2462.0
77	1914.0
78	1366.0
79	943.0
80	520.0
81	366.0
82	212.0
83	196.5
84	181.0
85	126.0
86	71.0
87	49.5
88	28.0
89	24.0
90	20.0
91	11.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	794237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.189857943033026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3191559556379	21.40175746848154
2	6.5560301895733195	3.827391798077622
3	3.079325812619375	2.696552490920229
4	2.106029084114974	2.4589875915684813
5	1.6127688345214335	2.353824658731579
6	1.3157595548964904	2.304410069677015
7	1.1041995147740749	2.2561998883914867
8	0.9472674017075019	2.2120480711926387
9	0.8739807835859589	2.2960237426033347
>10	8.866126271996478	52.956174463221195
>50	0.20024433869941838	3.590871684889943
>100	0.017809149381752723	0.915310286867193
>500	8.6873899423184E-4	0.1740851951611935
>1k	4.3436949711592E-4	0.5563625902165455
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4388	0.5524799272761154	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	854	0.10752458019457668	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02870679658590572	0.0
2	0.0	0.0	0.0	0.10903546422541383	0.0
3	0.0	0.0	0.0	0.1752625475771086	0.0
4	0.0	0.0	0.0	0.319929693529765	0.0
5	0.0	0.0	0.0	0.535734295934337	0.0
6	0.0	0.0	0.0	1.1113811116832886	0.0
7	0.0	0.0	0.0	1.4015967526065898	0.0
8	0.0	0.0	0.0	2.181968354533974	0.0
9	0.0	0.0	0.0	2.596076485985921	0.0
10	5.036280102790477E-4	0.0	0.0	3.1768854888402327	0.0
11	5.036280102790477E-4	0.0	0.0	3.5500738444570072	0.0
12	5.036280102790477E-4	0.0	0.0	3.845829393493378	0.0
13	5.036280102790477E-4	0.0	0.0	4.021595569080765	0.0
14	5.036280102790477E-4	0.0	0.0	4.125846567208528	0.0
15	5.036280102790477E-4	0.0	0.0	4.203783001799211	0.0
16	5.036280102790477E-4	0.0	0.0	4.3264164223021595	0.0
17	5.036280102790477E-4	0.0	0.0	4.450183005828235	0.0
18	5.036280102790477E-4	0.0	0.0	4.6389176026803085	0.0
19	5.036280102790477E-4	0.0	0.0	4.7373768786898625	0.0
20	5.036280102790477E-4	0.0	0.0	4.843894202863881	0.0
21	5.036280102790477E-4	0.0	0.0	4.9603581802409105	0.0
22	5.036280102790477E-4	0.0	0.0	5.085257926790114	0.0
23	5.036280102790477E-4	0.0	0.0	5.23017688674791	0.0
24	5.036280102790477E-4	0.0	0.0	5.351677144227731	0.0
25	5.036280102790477E-4	0.0	0.0	5.460586701450574	0.0
26	5.036280102790477E-4	0.0	0.0	5.572895747742802	0.0
27	5.036280102790477E-4	0.0	0.0	5.681931211968216	0.0
28	5.036280102790477E-4	0.0	0.0	5.786937652111398	0.0
29	5.036280102790477E-4	0.0	0.0	5.917125492768531	0.0
30	5.036280102790477E-4	0.0	0.0	6.093773017373907	0.0
31	5.036280102790477E-4	0.0	0.0	6.2288712311312615	0.0
32	5.036280102790477E-4	0.0	0.0	6.392046706461674	0.0
33	5.036280102790477E-4	0.0	0.0	6.521730919108528	0.0
34	5.036280102790477E-4	0.0	0.0	6.656073690850464	0.0
35	5.036280102790477E-4	0.0	0.0	6.800614929800551	0.0
36	5.036280102790477E-4	0.0	0.0	6.941630772678684	0.0
37	5.036280102790477E-4	0.0	0.0	7.080758010518271	0.0
38	5.036280102790477E-4	0.0	0.0	7.256524186105659	0.0
39	5.036280102790477E-4	0.0	0.0	7.533645498761705	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	20	7.0319674E-4	45.000004	42
CGTTCGA	20	7.0319674E-4	45.000004	14
TCTAACG	20	7.0319674E-4	45.000004	1
TATAGCG	40	6.8102963E-9	45.000004	1
TATACGA	20	7.0319674E-4	45.000004	38
GCGTTAG	35	1.2113014E-7	45.0	1
ATATACG	25	3.889713E-5	45.0	1
CGAATAT	110	0.0	45.0	14
TCGTAAG	25	3.889713E-5	45.0	1
TACGTAG	50	2.1827873E-11	45.0	1
CACGACG	30	2.164652E-6	44.999996	26
AGTTGCG	30	2.164652E-6	44.999996	1
CGGTCTA	30	2.164652E-6	44.999996	31
ATAGCGG	145	0.0	41.89655	2
CGTTTTT	2865	0.0	41.780106	1
TACGGGA	190	0.0	41.44737	4
TACGGGT	125	0.0	41.399998	4
ACGTAGG	120	0.0	41.249996	2
AATGCGG	95	0.0	40.263157	2
CGTAAGG	85	0.0	39.705883	2
>>END_MODULE