FastQCFastQC Report
Thu 26 May 2016
SRR1547687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547687_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1739447
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.3890316865072635No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39040.22443914646436483No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA207.034286E-445.00000445
ACGTTAG406.8193913E-945.0000041
CGTAGGT207.034286E-445.00000414
CTACGGT207.034286E-445.00000435
GTCCGAT207.034286E-445.00000438
TACTCGT207.034286E-445.00000411
CTCGATA207.034286E-445.00000424
TGTACCG207.034286E-445.00000434
GTTGCGA207.034286E-445.00000423
TCGCACA351.212411E-745.025
TCCGCAT253.8916347E-545.030
CTCCGAT253.8916347E-545.012
GCACGAC253.8916347E-545.039
TTATCCG351.212411E-745.01
CGTTACC253.8916347E-545.038
TTGATCG302.1661472E-644.99999620
ATAGCGC650.044.99999612
TATTCCG302.1661472E-644.9999961
TCGGTTA302.1661472E-644.99999635
TATCCGA302.1661472E-644.99999642