FastQCFastQC Report
Thu 26 May 2016
SRR1547686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547686_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1796730
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73350.4082416389774758No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50510.2811218157430443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG502.1827873E-1145.0000041
CGTACGT253.8916856E-545.00000432
CGTAATC253.8916856E-545.00000435
TTAGTCG253.8916856E-545.0000041
CGACTCA253.8916856E-545.00000431
CGTATAA207.0343504E-445.026
TAAGTCG207.0343504E-445.019
CACGTTA351.2124474E-745.029
TATTGCG1100.042.9545441
TACGTAG850.042.352941
CGTTTTT56000.041.7455371
TAAGTAG2950.041.1864361
CAATACG556.184564E-1140.9090921
ACGGGTA1250.039.6000025
TATAGCG1250.039.6000021
TATACGG2500.039.6000022
AATCGTG403.4596815E-739.37529
ATAGCGG4900.039.0306132
GACCGAT12000.039.09
GCGATAA2600.038.942319
Produced by FastQC (version 0.11.5)