##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547686_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1796730
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.416723158181806	31.0	31.0	33.0	30.0	34.0
2	31.82047942651372	31.0	31.0	34.0	30.0	34.0
3	31.94872574065107	31.0	31.0	34.0	30.0	34.0
4	35.6305727627412	37.0	35.0	37.0	33.0	37.0
5	35.4949430354032	37.0	35.0	37.0	33.0	37.0
6	35.54901237247667	37.0	35.0	37.0	33.0	37.0
7	35.97253176604164	37.0	35.0	37.0	35.0	37.0
8	35.996955023848884	37.0	35.0	37.0	35.0	37.0
9	37.745531604637314	39.0	37.0	39.0	35.0	39.0
10	37.16043312016831	39.0	37.0	39.0	33.0	39.0
11	36.83930251067216	39.0	35.0	39.0	32.0	39.0
12	35.83471974086257	37.0	35.0	39.0	32.0	39.0
13	35.33446984243598	37.0	35.0	39.0	30.0	39.0
14	36.26453112042433	38.0	35.0	41.0	31.0	41.0
15	36.61248490312958	38.0	35.0	41.0	31.0	41.0
16	36.80666822505329	38.0	35.0	41.0	32.0	41.0
17	36.767598915808165	38.0	35.0	41.0	32.0	41.0
18	36.678629510277005	38.0	35.0	40.0	32.0	41.0
19	36.604636200208155	37.0	35.0	40.0	32.0	41.0
20	36.46963817601977	37.0	35.0	40.0	31.0	41.0
21	36.312502713262425	37.0	35.0	40.0	31.0	41.0
22	36.19697561681499	37.0	35.0	40.0	31.0	41.0
23	36.14560785426859	37.0	35.0	40.0	31.0	41.0
24	36.02379155465763	36.0	35.0	40.0	31.0	41.0
25	35.86411202573564	36.0	35.0	40.0	31.0	41.0
26	35.685354505128764	36.0	34.0	40.0	30.0	41.0
27	35.605594607982276	36.0	34.0	40.0	30.0	41.0
28	35.692726786996374	36.0	34.0	40.0	30.0	41.0
29	35.71565844617722	36.0	35.0	40.0	30.0	41.0
30	35.736966043868584	36.0	35.0	40.0	30.0	41.0
31	35.5088098935288	36.0	34.0	40.0	30.0	41.0
32	35.2721260289526	35.0	34.0	40.0	29.0	41.0
33	35.12653876765012	36.0	34.0	40.0	29.0	41.0
34	34.95571176526245	36.0	34.0	40.0	27.0	41.0
35	34.81368764366377	35.0	34.0	40.0	27.0	41.0
36	34.591788415621714	35.0	34.0	40.0	25.0	41.0
37	34.31230680180105	35.0	33.0	40.0	24.0	41.0
38	34.41424921941527	35.0	33.0	40.0	25.0	41.0
39	34.34108575022402	35.0	33.0	40.0	24.0	41.0
40	34.14886098634742	35.0	33.0	40.0	23.0	41.0
41	34.11956944003829	35.0	33.0	40.0	23.0	41.0
42	34.0555247588675	35.0	33.0	40.0	23.0	41.0
43	33.941030650125505	35.0	33.0	40.0	23.0	41.0
44	33.77202028128879	35.0	33.0	39.0	23.0	41.0
45	33.68420575155978	35.0	33.0	39.0	23.0	41.0
46	33.54104178145854	35.0	33.0	39.0	23.0	41.0
47	33.56256421387743	35.0	33.0	39.0	23.0	41.0
48	33.433262092801925	35.0	33.0	39.0	23.0	41.0
49	33.458847461777786	35.0	33.0	39.0	23.0	41.0
50	33.28587378181474	35.0	33.0	39.0	23.0	41.0
51	32.571253889009476	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	5.0
13	5.0
14	21.0
15	66.0
16	196.0
17	477.0
18	1130.0
19	2153.0
20	3508.0
21	5603.0
22	8418.0
23	12229.0
24	17572.0
25	23919.0
26	29902.0
27	35190.0
28	39071.0
29	43932.0
30	51423.0
31	60947.0
32	75504.0
33	98836.0
34	177365.0
35	305396.0
36	125510.0
37	147084.0
38	205742.0
39	325307.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.92434589504266	25.089022835929715	30.29420113205657	13.692430136971053
2	29.234999137321687	27.23096959476382	29.665614755695067	13.86841651221942
3	27.583888508568343	25.705587372615806	32.653598481686174	14.056925637129675
4	24.23530524898009	30.23492678365698	30.58144518096765	14.948322786395284
5	22.82151464048577	33.049094744341105	29.54094382572785	14.58844678944527
6	21.158827425378327	41.90223350197303	25.622825911517033	11.31611316113161
7	86.63594418749618	5.306250800064562	6.34563902200108	1.7121659904381847
8	88.0569701624618	3.6448436882558872	5.527708670751866	2.770477478530441
9	83.03451269806816	5.47461221218547	8.45797643496797	3.032898654778403
10	54.695697183216176	19.214461827876196	13.267658468439889	12.82218252046774
11	48.46114886488231	19.657321912585644	18.101217211267134	13.78031201126491
12	41.859322213131634	20.858225776827904	22.09541778675705	15.18703422328341
13	22.03742354165623	39.74019468701474	23.896077874805897	14.326303896523129
14	16.36177945489862	42.090019090236154	26.89135262393348	14.656848830931748
15	13.98407106243008	23.19970168027472	48.227836124515086	14.588391132780107
16	16.33985072882403	17.496952797582274	47.20904086868923	18.954155604904464
17	17.947493502084342	18.5449678026192	27.114090597919553	36.393448097376904
18	22.692892087291913	22.53237826495912	33.15534331813906	21.619386329609902
19	28.61815631730978	24.879753774913315	24.98822861531783	21.513861292459076
20	30.885274916097575	24.239702125528044	23.138145408603407	21.736877549770973
21	23.514328808446454	28.893378526545447	27.064723135919145	20.527569529088957
22	23.822555420124335	24.6055333856506	25.194102619759228	26.37780857446583
23	21.182314538077506	30.941376834582822	22.554473961029203	25.321834666310465
24	22.00458610920951	24.833558742827247	34.8617210154002	18.300134132563045
25	18.24085978416345	25.22632782888915	33.387876865193995	23.144935521753407
26	17.221841901676935	33.433125733972275	26.772915240464624	22.57211712388617
27	19.324439398240138	31.80600312790458	27.973707791376555	20.895849682478726
28	16.442537276051496	28.42040818598231	36.766904320626914	18.370150217339276
29	17.097727538361358	24.816973056608393	35.79998107673384	22.285318328296405
30	20.477756813767233	28.80666544222003	30.655802485626666	20.05977525838607
31	29.114112860585617	25.85953370845926	24.227847255848122	20.798506175107
32	29.8566284305377	26.567764772670348	24.802836263656754	18.772770533135198
33	27.857774957840075	26.93815987933635	24.57770505306863	20.626360109754945
34	20.697767611160277	26.843487891892494	28.334641264964688	24.124103231982545
35	21.388856422500876	25.282262777378904	29.9618751843627	23.367005615757517
36	31.18292676139431	22.06697723085828	26.66388383340847	20.08621217433894
37	21.535344765212358	28.169897536079436	28.956994094827827	21.337763603880383
38	22.452399637118543	29.910726709077046	23.729887072626383	23.90698658117803
39	20.877037729653313	28.884863056775362	25.98047564186049	24.25762357171083
40	24.22651149588419	24.65345377435675	24.661746617466175	26.458288112292887
41	18.376884673824115	22.100371229956643	26.670451319897815	32.85229277632143
42	23.595754509581297	24.96145775937397	24.03349418109566	27.409293549949076
43	24.008838278428033	25.13399342138218	25.46047541923383	25.396692880955957
44	22.565605294061992	28.558269745593385	27.01919598381504	21.856928976529584
45	19.279635782783167	33.65063198143294	22.983308566117337	24.08642366966656
46	23.69365458360466	29.570386201599575	26.28235739370968	20.453601821086085
47	23.52963439136654	26.177054983219517	26.5947025986097	23.698608026804248
48	25.073939879670288	23.01575640190791	28.463263818158545	23.447039900263256
49	22.412271181535345	20.74886042978077	31.092762963828736	25.746105424855152
50	20.999204109688154	28.974358974358978	26.741747507972818	23.284689407980053
51	20.28173403905985	31.701758194052527	23.135306918680048	24.881200848207577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1701.0
1	2006.0
2	2311.0
3	7095.0
4	11879.0
5	8767.5
6	5656.0
7	6512.0
8	7368.0
9	8562.0
10	9756.0
11	10418.0
12	11080.0
13	11420.5
14	11761.0
15	11393.5
16	11026.0
17	10472.5
18	9919.0
19	9300.5
20	8682.0
21	8939.0
22	9196.0
23	8497.0
24	7798.0
25	7707.0
26	7836.5
27	8057.0
28	8751.5
29	9446.0
30	11372.0
31	13298.0
32	16805.0
33	20312.0
34	23814.0
35	27316.0
36	30032.0
37	32748.0
38	42835.5
39	52923.0
40	78017.0
41	103111.0
42	131913.5
43	160716.0
44	164533.0
45	168350.0
46	156798.0
47	145246.0
48	147246.5
49	149247.0
50	154999.5
51	160752.0
52	151956.0
53	143160.0
54	125288.5
55	107417.0
56	96048.0
57	84679.0
58	78230.0
59	71781.0
60	64660.0
61	57539.0
62	52503.0
63	47467.0
64	41024.0
65	34581.0
66	31633.0
67	28685.0
68	23838.0
69	18991.0
70	16179.0
71	13367.0
72	11282.0
73	9197.0
74	7398.5
75	4287.0
76	2974.0
77	2525.0
78	2076.0
79	1456.0
80	836.0
81	762.0
82	688.0
83	479.5
84	271.0
85	173.5
86	76.0
87	51.5
88	27.0
89	22.5
90	18.0
91	15.0
92	12.0
93	13.0
94	14.0
95	7.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1796730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.535748317113466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.37013178152264	17.739024268943407
2	7.126187784294356	3.0693557316610347
3	2.269413944977154	1.4662058263912674
4	1.1298915657173079	0.9733224153966886
5	0.6768098240292016	0.728780301442137
6	0.4990472684921166	0.6448413823553503
7	0.35220005435082463	0.530942420944113
8	0.29139499832484733	0.502032747583169
9	0.2243558708528145	0.43485044113377236
>10	2.6026200617373276	13.47614865618049
>50	1.2265021401211709	19.33559952768539
>100	1.226498098940868	39.53712689599226
>500	0.0028638248965328966	0.4521870430553353
>1k	0.0015620863071997618	0.4151262079084566
>5k	5.206954357332539E-4	0.6944561333271398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7335	0.4082416389774758	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5051	0.2811218157430443	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1131333032787341E-4	0.0	0.0	0.05799424510082205	0.0
2	1.1131333032787341E-4	0.0	0.0	0.2428856867754198	0.0
3	1.1131333032787341E-4	0.0	0.0	0.352640630478703	0.0
4	1.1131333032787341E-4	0.0	0.0	0.5938009606340408	0.0
5	1.1131333032787341E-4	0.0	0.0	0.9399297612885631	0.0
6	1.1131333032787341E-4	0.0	0.0	1.655841445292281	0.0
7	1.1131333032787341E-4	0.0	0.0	1.9920077028824588	0.0
8	1.1131333032787341E-4	0.0	0.0	2.9407868739320877	0.0
9	1.1131333032787341E-4	0.0	0.0	3.5020843421103893	0.0
10	1.1131333032787341E-4	0.0	0.0	4.385188648266573	0.0
11	1.1131333032787341E-4	0.0	0.0	5.114179648583816	0.0
12	1.1131333032787341E-4	0.0	0.0	5.706589192588758	0.0
13	1.1131333032787341E-4	0.0	0.0	6.005131544528115	0.0
14	1.1131333032787341E-4	0.0	0.0	6.132696621083858	0.0
15	1.1131333032787341E-4	0.0	0.0	6.263879380875257	0.0
16	1.1131333032787341E-4	0.0	0.0	6.498806164532233	0.0
17	1.1131333032787341E-4	0.0	0.0	6.735235678148637	0.0
18	1.6696999549181013E-4	0.0	0.0	7.105241188158488	0.0
19	1.6696999549181013E-4	0.0	0.0	7.255402870770789	0.0
20	3.3393999098362025E-4	0.0	0.0	7.41502618646096	0.0
21	3.3393999098362025E-4	0.0	0.0	7.608878351226951	0.0
22	3.8959665614755694E-4	0.0	0.0	7.793435852910565	0.0
23	3.8959665614755694E-4	0.0	0.0	7.99552520412082	0.0
24	3.8959665614755694E-4	0.0	0.0	8.153033566534761	0.0
25	3.8959665614755694E-4	0.0	0.0	8.283437133013864	0.0
26	3.8959665614755694E-4	0.0	0.0	8.410946552904443	0.0
27	8.905066426229873E-4	0.0	0.0	8.545301742610187	0.0
28	9.461633077869241E-4	0.0	0.0	8.671698029197486	0.0
29	0.0010018199729508607	0.0	0.0	8.80165634235528	0.0
30	0.0010018199729508607	0.0	0.0	9.00341175357455	0.0
31	0.0010018199729508607	0.0	0.0	9.151180199584802	0.0
32	0.0010018199729508607	0.0	0.0	9.277186889515955	0.0
33	0.0010018199729508607	0.0	0.0	9.40647732269178	0.0
34	0.0010018199729508607	0.0	0.0	9.538494932460637	0.0
35	0.0010018199729508607	0.0	0.0	9.708804327862284	0.0
36	0.0010018199729508607	0.0	0.0	9.840265370979502	0.0
37	0.0010018199729508607	0.0	0.0	9.985473610392212	0.0
38	0.0010018199729508607	0.0	0.0	10.129457403171317	0.0
39	0.0010018199729508607	0.0	0.0	10.32586977453485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCACG	50	2.1827873E-11	45.000004	1
CGTACGT	25	3.8916856E-5	45.000004	32
CGTAATC	25	3.8916856E-5	45.000004	35
TTAGTCG	25	3.8916856E-5	45.000004	1
CGACTCA	25	3.8916856E-5	45.000004	31
CGTATAA	20	7.0343504E-4	45.0	26
TAAGTCG	20	7.0343504E-4	45.0	19
CACGTTA	35	1.2124474E-7	45.0	29
TATTGCG	110	0.0	42.954544	1
TACGTAG	85	0.0	42.35294	1
CGTTTTT	5600	0.0	41.745537	1
TAAGTAG	295	0.0	41.186436	1
CAATACG	55	6.184564E-11	40.909092	1
ACGGGTA	125	0.0	39.600002	5
TATAGCG	125	0.0	39.600002	1
TATACGG	250	0.0	39.600002	2
AATCGTG	40	3.4596815E-7	39.375	29
ATAGCGG	490	0.0	39.030613	2
GACCGAT	1200	0.0	39.0	9
GCGATAA	260	0.0	38.94231	9
>>END_MODULE