FastQCFastQC Report
Thu 26 May 2016
SRR1547685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547685_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences549597
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.31768732362076213No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17430.31714146911282265No Hit
GAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT5510.10025527795821303No Hit
GAGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT5500.10007332645556653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG351.2103737E-745.0000041
ACACGTG700.045.00000442
ACGCCGA253.8881386E-545.00000412
ACCGCTC253.8881386E-545.00000418
TTGAGCG253.8881386E-545.0000041
GTTACAC253.8881386E-545.00000433
GGGCGTA253.8881386E-545.0000047
GTCAACG253.8881386E-545.0000041
CGAATAT253.8881386E-545.00000414
CGTACCA253.8881386E-545.00000413
TAAGGAG351.2103737E-745.0000041
ATAACGG253.8881386E-545.0000042
ATGCACC253.8881386E-545.00000427
GCGTACC253.8881386E-545.00000412
TGCGTTG253.8881386E-545.0000041
AGTCGTG253.8881386E-545.00000412
CCCGAGT253.8881386E-545.00000418
TTTAGCG406.8030204E-945.01
TCGTTAG207.03007E-445.01
CGAAAGA207.03007E-445.042