##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1547685_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 549597 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.447609794085484 31.0 31.0 33.0 30.0 34.0 2 31.84665673211462 31.0 31.0 34.0 30.0 34.0 3 31.98509999144828 33.0 31.0 34.0 30.0 34.0 4 35.65161745788278 37.0 35.0 37.0 33.0 37.0 5 35.530566942687095 37.0 35.0 37.0 33.0 37.0 6 35.572235656308166 37.0 35.0 37.0 33.0 37.0 7 35.98388091638055 37.0 35.0 37.0 35.0 37.0 8 36.00954153679878 37.0 35.0 37.0 35.0 37.0 9 37.776065007632866 39.0 38.0 39.0 35.0 39.0 10 37.20650767744365 39.0 37.0 39.0 33.0 39.0 11 36.862310019887296 39.0 35.0 39.0 33.0 39.0 12 35.7433210152166 37.0 35.0 39.0 32.0 39.0 13 35.18467167761105 37.0 35.0 39.0 30.0 39.0 14 36.12537550241359 38.0 35.0 41.0 31.0 41.0 15 36.52281944770441 38.0 35.0 41.0 31.0 41.0 16 36.74746041190181 38.0 35.0 41.0 32.0 41.0 17 36.70130477422548 38.0 35.0 40.0 32.0 41.0 18 36.65349155836004 38.0 35.0 40.0 32.0 41.0 19 36.58480122708093 37.0 35.0 40.0 32.0 41.0 20 36.448339419611095 37.0 35.0 40.0 32.0 41.0 21 36.289799616810136 37.0 35.0 40.0 31.0 41.0 22 36.1726574198913 37.0 35.0 40.0 31.0 41.0 23 36.13377438377575 36.0 35.0 40.0 31.0 41.0 24 36.01984908942371 36.0 35.0 40.0 31.0 41.0 25 35.87236284040852 36.0 35.0 40.0 31.0 41.0 26 35.656220830899734 35.0 34.0 40.0 30.0 41.0 27 35.627989235749105 35.0 34.0 40.0 30.0 41.0 28 35.73897601333341 36.0 35.0 40.0 30.0 41.0 29 35.820173690904426 36.0 35.0 40.0 30.0 41.0 30 35.85726086568886 36.0 35.0 40.0 31.0 41.0 31 35.67506918705888 36.0 35.0 40.0 30.0 41.0 32 35.4286286133294 35.0 34.0 40.0 30.0 41.0 33 35.36113188390766 36.0 34.0 40.0 30.0 41.0 34 35.23613120158953 36.0 34.0 40.0 29.0 41.0 35 35.16479711497698 36.0 34.0 40.0 29.0 41.0 36 34.959106399780204 35.0 34.0 40.0 27.0 41.0 37 34.66041663255076 35.0 34.0 40.0 26.0 41.0 38 34.7781301571879 35.0 34.0 40.0 27.0 41.0 39 34.707725842753874 35.0 34.0 40.0 27.0 41.0 40 34.55855472282418 35.0 34.0 40.0 26.0 41.0 41 34.55968282214059 35.0 34.0 40.0 26.0 41.0 42 34.53754842184364 35.0 34.0 40.0 26.0 41.0 43 34.424176260059646 35.0 34.0 40.0 26.0 41.0 44 34.28502702889572 35.0 34.0 40.0 26.0 41.0 45 34.234999463243064 35.0 34.0 40.0 26.0 41.0 46 34.094325478486965 35.0 34.0 40.0 24.0 41.0 47 34.13931116800128 35.0 33.0 40.0 26.0 41.0 48 34.032869538953086 35.0 33.0 39.0 24.0 41.0 49 34.07792618955344 35.0 34.0 39.0 25.0 41.0 50 33.895541642330656 35.0 33.0 39.0 24.0 41.0 51 33.15884912035546 35.0 32.0 38.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 2.0 14 4.0 15 8.0 16 50.0 17 134.0 18 264.0 19 563.0 20 980.0 21 1554.0 22 2246.0 23 3294.0 24 4543.0 25 6242.0 26 8002.0 27 9478.0 28 10580.0 29 12413.0 30 14883.0 31 17862.0 32 22804.0 33 30188.0 34 55931.0 35 101836.0 36 36293.0 37 41073.0 38 60060.0 39 108246.0 40 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.497806574635597 25.107851753193707 29.990338375209475 13.404003296961228 2 27.8591404247112 26.85021934253644 31.598243804096455 13.692396428655906 3 27.709212386530496 26.699381546842503 31.66647561758889 13.924930449038115 4 24.138778050098527 30.25435000554952 30.653369650853264 14.95350229349869 5 22.33691231939039 34.135557508501684 29.408275518243364 14.11925465386456 6 21.272132125903163 40.88886948072861 26.634788763403 11.204209629965229 7 87.98337691071822 4.5888168967443415 5.748757726115681 1.67904846642176 8 89.15023189719012 3.243649437678881 4.8166201780577405 2.7894984870732555 9 83.97480335591352 5.052611276990231 7.878863967598076 3.093721399498178 10 54.996661189926435 22.081452409674725 11.259340935267113 11.662545465131725 11 50.62418462982876 18.570698166110805 18.921136760207936 11.883980443852495 12 43.72622121299789 20.22518317967529 21.587272128486873 14.46132347883995 13 20.06251853630933 44.76116136005109 22.923160060917365 12.253160042722213 14 14.791929359148611 45.85232452142206 25.249773925257962 14.105972194171365 15 12.033908482033198 22.83891651519204 51.409305363748345 13.717869639026414 16 13.96241245858329 17.3701821516493 49.53975367405572 19.127651715711693 17 14.093781443494052 18.786310696746888 27.520164775280797 39.59974308447826 18 21.85492278887985 22.17897841509324 34.28166456512681 21.684434230900095 19 28.765804762398627 26.036168319696067 22.968284033573692 22.22974288433161 20 31.434305500211973 23.80835412129251 22.7917910760066 21.965549302488917 21 21.50721346732242 30.5791334377735 25.54799243809555 22.365660656808533 22 22.942992774705832 25.727396619704983 23.842015149282112 27.487595456307073 23 19.081617985542135 32.399012367243635 22.214458958109308 26.304910689104926 24 20.584355445899448 23.85911859053088 38.287144944386526 17.269381019183147 25 16.380365977252424 26.312188749210787 35.12792100393561 22.17952426960118 26 16.330693217029932 36.60063646635626 25.566369539862844 21.502300776750964 27 17.935505470371925 33.25636784771387 27.910086845452213 20.89803983646199 28 14.52245918372917 29.326397342052452 38.52713897637724 17.624004497841145 29 14.726790721201171 24.0185081068492 38.47309938009123 22.781601791858396 30 18.118730633536938 30.48815768645025 31.26363499072957 20.129476689283237 31 29.602053868561875 26.91681359250505 23.144231136632843 20.33690140230023 32 30.327858412618703 28.2212239149777 23.780697492890248 17.67022017951335 33 27.270709265152465 27.326568376464937 24.269783132003997 21.1329392263786 34 18.61654994477772 28.0090684628919 28.01325334745277 25.361128244877612 35 19.439152688242476 25.388239018771934 31.30239066079327 23.870217632192315 36 31.20686612190387 22.889317081425116 25.748139091006685 20.15567770566433 37 20.281952048500994 29.81020638758945 30.63790377312831 19.269937790781245 38 20.961540910885613 31.317674587015574 24.457557082735168 23.263227419363645 39 19.485186418412038 30.863705587912598 28.107686177326297 21.54342181634907 40 23.49066679767175 25.108215656198997 25.5325265603706 25.868590985758654 41 16.394376242956202 22.511221858925722 29.002705618844356 32.09169627927373 42 23.043066101161397 26.191736854458814 24.289069991284524 26.476127053095265 43 22.773232022736657 25.98958873501857 27.01943423999767 24.217745002247103 44 21.200989088368384 30.875714387087267 26.83893834937236 21.08435817517199 45 17.099074412706035 36.792231398643004 22.084545585219715 24.024148603431243 46 22.95118059232492 31.35970538412691 25.602213985884205 20.08690003766396 47 22.023955734838434 26.594577481318126 27.629517628371335 23.7519491554721 48 24.025604215452415 21.953904406319538 30.240703642851035 23.779787735377013 49 20.878207122673523 21.378937657956648 32.379361604957815 25.363493614412015 50 19.028852049774652 31.340782427851682 27.467580791015962 22.162784731357704 51 17.954246475144515 33.420488103101 23.681351972445263 24.94391344930922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 459.0 1 554.5 2 650.0 3 1763.0 4 2876.0 5 2113.0 6 1350.0 7 1541.0 8 1732.0 9 2050.5 10 2369.0 11 2537.5 12 2706.0 13 2720.0 14 2734.0 15 2611.5 16 2489.0 17 2504.5 18 2520.0 19 2405.0 20 2290.0 21 2295.5 22 2301.0 23 2283.5 24 2266.0 25 2305.5 26 3043.5 27 3742.0 28 4052.5 29 4363.0 30 5204.5 31 6046.0 32 6974.0 33 7902.0 34 8689.0 35 9476.0 36 11138.0 37 12800.0 38 16492.5 39 20185.0 40 29800.0 41 39415.0 42 47273.5 43 55132.0 44 57538.5 45 59945.0 46 58393.5 47 56842.0 48 55026.5 49 53211.0 50 48966.5 51 44722.0 52 40629.5 53 36537.0 54 31353.5 55 26170.0 56 23285.0 57 20400.0 58 18008.0 59 15616.0 60 14614.0 61 13612.0 62 12493.0 63 11374.0 64 9736.5 65 8099.0 66 6770.5 67 5442.0 68 4389.0 69 3336.0 70 2854.5 71 2373.0 72 1963.5 73 1554.0 74 1263.5 75 813.5 76 654.0 77 464.0 78 274.0 79 219.5 80 165.0 81 117.5 82 70.0 83 54.0 84 38.0 85 30.5 86 23.0 87 15.0 88 7.0 89 7.5 90 8.0 91 5.5 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 549597.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.44811365033614 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14889191986404 19.350411049550075 2 5.723588661886453 2.798618921871507 3 2.1125407329952113 1.54942907793694 4 1.2447814923919693 1.2173023758333559 5 0.8290593511779992 1.0134468620236834 6 0.6169541023110631 0.9050018406205187 7 0.560636251897357 0.9594549162019544 8 0.4785301671051917 0.9359327928401656 9 0.4076934132060453 0.8970601410499364 >10 7.490312665021421 46.84255880023234 >50 1.3191885537848074 20.418405797419023 >100 0.06484147128772783 2.2760205145153494 >500 0.0014906085353500647 0.20061633067659912 >1k 0.0014906085353500647 0.6357405792285689 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1746 0.31768732362076213 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1743 0.31714146911282265 No Hit GAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT 551 0.10025527795821303 No Hit GAGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT 550 0.10007332645556653 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.04767129369337897 0.0 2 0.0 0.0 0.0 0.20360373146141628 0.0 3 0.0 0.0 0.0 0.32059854766310586 0.0 4 0.0 0.0 0.0 0.6424707558447371 0.0 5 0.0 0.0 0.0 1.0325747775188001 0.0 6 0.0 0.0 0.0 1.9150395653542505 0.0 7 0.0 0.0 0.0 2.331526554912054 0.0 8 0.0 0.0 0.0 3.5375011144529536 0.0 9 0.0 0.0 0.0 4.129935207069908 0.0 10 0.0 0.0 0.0 4.974917985360182 0.0 11 0.0 0.0 0.0 5.539149595066931 0.0 12 0.0 0.0 0.0 6.022594737598641 0.0 13 0.0 0.0 0.0 6.283513192393699 0.0 14 0.0 0.0 0.0 6.393593851494822 0.0 15 0.0 0.0 0.0 6.487844729865701 0.0 16 0.0 0.0 0.0 6.666339153961903 0.0 17 0.0 0.0 0.0 6.841558451010468 0.0 18 0.0 0.0 0.0 7.147236975456562 0.0 19 0.0 0.0 0.0 7.25295079849417 0.0 20 0.0 0.0 0.0 7.36885390567998 0.0 21 0.0 0.0 0.0 7.512413641268056 0.0 22 0.0 0.0 0.0 7.662887533956699 0.0 23 0.0 0.0 0.0 7.850479533185225 0.0 24 0.0 0.0 0.0 7.976389973016592 0.0 25 0.0 0.0 0.0 8.088472098646827 0.0 26 0.0 0.0 0.0 8.191092746139445 0.0 27 0.0 0.0 0.0 8.288982654563252 0.0 28 0.0 0.0 0.0 8.394150623092921 0.0 29 0.0 0.0 0.0 8.502957621675518 0.0 30 0.0 0.0 0.0 8.689094008882872 0.0 31 0.0 0.0 0.0 8.816460060735412 0.0 32 0.0 0.0 0.0 8.926358768333888 0.0 33 0.0 0.0 0.0 9.02770575530798 0.0 34 0.0 0.0 0.0 9.146883989541427 0.0 35 0.0 0.0 0.0 9.306455457362395 0.0 36 0.0 0.0 0.0 9.432183945691115 0.0 37 0.0 0.0 0.0 9.559368046041008 0.0 38 0.0 0.0 0.0 9.67054041415801 0.0 39 0.0 0.0 0.0 9.807913798656106 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTTG 35 1.2103737E-7 45.000004 1 ACACGTG 70 0.0 45.000004 42 ACGCCGA 25 3.8881386E-5 45.000004 12 ACCGCTC 25 3.8881386E-5 45.000004 18 TTGAGCG 25 3.8881386E-5 45.000004 1 GTTACAC 25 3.8881386E-5 45.000004 33 GGGCGTA 25 3.8881386E-5 45.000004 7 GTCAACG 25 3.8881386E-5 45.000004 1 CGAATAT 25 3.8881386E-5 45.000004 14 CGTACCA 25 3.8881386E-5 45.000004 13 TAAGGAG 35 1.2103737E-7 45.000004 1 ATAACGG 25 3.8881386E-5 45.000004 2 ATGCACC 25 3.8881386E-5 45.000004 27 GCGTACC 25 3.8881386E-5 45.000004 12 TGCGTTG 25 3.8881386E-5 45.000004 1 AGTCGTG 25 3.8881386E-5 45.000004 12 CCCGAGT 25 3.8881386E-5 45.000004 18 TTTAGCG 40 6.8030204E-9 45.0 1 TCGTTAG 20 7.03007E-4 45.0 1 CGAAAGA 20 7.03007E-4 45.0 42 >>END_MODULE