FastQCFastQC Report
Thu 26 May 2016
SRR1547684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547684_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5810637
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1593142.741764801346221No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT73350.1262340084228287No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC73030.12568329427565345No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT62730.10795718266344981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT708050.044.1833231
CGTTCGG15400.041.201345
GCGTAAG4900.040.4081651
GTTTTTT786100.040.0139922
ATCGCCG451.9306754E-840.010
ACGGGTA6700.038.9552235
TGATTCG9950.038.8944715
CGCATCG1000.038.25000421
AGGGCGA65000.038.256
TACGGGA16550.038.202424
CGTAAGG9750.038.0769272
TAGACGG6450.037.674422
ATTATAC29300.037.55119742
ACGCGAG1500.037.51
TGGGCGA41550.037.418776
GCCCTAC31050.037.39130426
TTCGGCA10600.037.3584918
TAGTACG2650.037.358491
GTTAGCG3200.037.265631
GGCGATA34950.036.8884128
Produced by FastQC (version 0.11.5)