FastQCFastQC Report
Thu 26 May 2016
SRR1547683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547683_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2445775
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83330.3407099998977829No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48690.199078001860351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG351.2126839E-745.0000041
CGTTTTT59550.040.7304761
GGCGATA11450.040.4803478
AGGGCGA32850.039.7260256
TACGGAC403.4603545E-739.3750044
TATGGGT11900.038.949584
TAGGGTA15700.038.8375785
GCGATAA2500.038.6999979
TTCGTAC356.251179E-638.5714333
GGGCGAT61600.038.3887987
TAGGGCG13700.038.102195
TCACGGG5900.037.754243
ACACGAC7000.037.60714326
ATAGGGT13600.037.5551454
AACGTAT301.1402982E-437.50000416
CGCGTAG301.1402982E-437.5000041
TAGGGTC11600.037.4353455
TAGGGAC16050.037.429915
GACACGA7050.037.34042725
ATAGGGC15000.036.8999984
Produced by FastQC (version 0.11.5)