##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547683_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2445775
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.429142909711647	31.0	31.0	33.0	30.0	34.0
2	31.846295346055953	31.0	31.0	34.0	30.0	34.0
3	32.00542159438215	33.0	31.0	34.0	30.0	34.0
4	35.690077787204466	37.0	35.0	37.0	33.0	37.0
5	35.56465373961219	37.0	35.0	37.0	33.0	37.0
6	35.617858551992725	37.0	35.0	37.0	33.0	37.0
7	36.01543069170304	37.0	35.0	37.0	35.0	37.0
8	36.02872995267349	37.0	35.0	37.0	35.0	37.0
9	37.782187241262996	39.0	38.0	39.0	35.0	39.0
10	37.09820527235743	39.0	37.0	39.0	33.0	39.0
11	36.90611810162423	39.0	37.0	39.0	33.0	39.0
12	36.26952561049156	38.0	35.0	39.0	32.0	39.0
13	36.00220666250984	38.0	35.0	39.0	31.0	39.0
14	37.018963314286886	39.0	35.0	41.0	31.0	41.0
15	37.22904314583312	39.0	35.0	41.0	32.0	41.0
16	37.309575492430824	39.0	35.0	41.0	32.0	41.0
17	37.26093610409788	39.0	35.0	41.0	32.0	41.0
18	37.19531150657767	39.0	35.0	41.0	32.0	41.0
19	37.15595547423618	39.0	35.0	41.0	32.0	41.0
20	37.06498594515031	39.0	35.0	41.0	32.0	41.0
21	36.959537978759286	38.0	35.0	41.0	32.0	41.0
22	36.85458883176089	38.0	35.0	41.0	31.0	41.0
23	36.77044985740716	38.0	35.0	41.0	31.0	41.0
24	36.68061902668888	38.0	35.0	41.0	31.0	41.0
25	36.55006449898294	38.0	35.0	40.0	31.0	41.0
26	36.38785170344778	38.0	35.0	40.0	31.0	41.0
27	36.341111099753654	38.0	35.0	40.0	31.0	41.0
28	36.38914658952684	38.0	35.0	40.0	31.0	41.0
29	36.42353487135979	38.0	35.0	40.0	31.0	41.0
30	36.458550357248726	38.0	35.0	40.0	31.0	41.0
31	36.33367092230479	38.0	35.0	40.0	31.0	41.0
32	36.14200774805532	38.0	35.0	40.0	30.0	41.0
33	36.06212550214145	38.0	35.0	40.0	30.0	41.0
34	35.89024297001973	38.0	35.0	40.0	30.0	41.0
35	35.82368083736239	38.0	35.0	40.0	30.0	41.0
36	35.66844374482526	38.0	35.0	40.0	29.0	41.0
37	35.400159867526654	38.0	34.0	40.0	27.0	41.0
38	35.48688820517014	38.0	34.0	40.0	29.0	41.0
39	35.39785098792816	37.0	34.0	40.0	28.0	41.0
40	35.23774590876103	37.0	34.0	40.0	27.0	41.0
41	35.21595567867036	37.0	34.0	40.0	27.0	41.0
42	35.18506853655794	37.0	34.0	40.0	27.0	41.0
43	35.09775674377242	37.0	34.0	40.0	27.0	41.0
44	34.94906236264579	37.0	34.0	40.0	27.0	41.0
45	34.87377743251117	37.0	34.0	40.0	27.0	41.0
46	34.694551215872266	36.0	34.0	40.0	26.0	41.0
47	34.73557420449551	36.0	34.0	40.0	26.0	41.0
48	34.59281209432593	36.0	34.0	40.0	26.0	41.0
49	34.58381985260296	36.0	34.0	40.0	26.0	41.0
50	34.42357494045854	36.0	34.0	40.0	26.0	41.0
51	33.70288027312406	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	8.0
13	6.0
14	15.0
15	68.0
16	160.0
17	406.0
18	1012.0
19	1930.0
20	3389.0
21	5436.0
22	8248.0
23	12038.0
24	17373.0
25	23967.0
26	31303.0
27	38122.0
28	43034.0
29	50817.0
30	60164.0
31	73184.0
32	91773.0
33	119406.0
34	201836.0
35	320512.0
36	183214.0
37	229639.0
38	336911.0
39	591445.0
40	356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.25591070315135	24.221483987693063	30.250411423781827	13.272193885373756
2	29.055657204771496	26.64819944598338	30.440944077030796	13.855199272214328
3	28.222710592756894	26.198566916417086	31.353211149840032	14.225511340985985
4	25.11473868201286	29.11747809998876	30.27522973290675	15.492553485091637
5	22.97758379245842	33.54936574296491	29.089061749343255	14.383988715233412
6	21.49686704623279	41.31778270691294	26.089194631558506	11.096155615295764
7	89.49425028876328	3.862661119685989	5.138984575441322	1.5041040161094132
8	90.09091187864787	2.9580807719434534	4.671934253968579	2.2790730954400957
9	84.79079228465415	4.345166664963049	7.731250830513845	3.1327902198689577
10	44.437489139434334	21.00696098373726	19.43976858051129	15.115781296317119
11	40.47571833059051	23.00403757500179	20.14895074158498	16.371293352822725
12	35.544193558279076	23.027302184379185	23.038382516789156	18.390121740552587
13	22.945896494976033	33.67313019390581	25.139311670125014	18.241661640993144
14	18.967280309922213	35.95878606985516	27.241181220676474	17.832752399546155
15	18.943647719025666	23.880651327288895	39.99153642505954	17.184164528625896
16	19.882123253365496	20.89730249103045	39.117294109229185	20.10328014637487
17	20.610195132422238	21.142337295949137	27.261256656887902	30.986210914740724
18	24.493422330345187	23.380319121750777	30.35904774560211	21.76721080230193
19	28.053725301795957	25.225010477251587	25.177867955964878	21.543396264987578
20	29.67828193517392	24.746675389191566	23.864705461459046	21.710337214175464
21	25.008105815130172	28.06059429015343	25.715407181772648	21.21589271294375
22	24.602999049381076	24.33952428166941	25.59282844906011	25.4646482198894
23	22.731158835134057	28.841819055309664	24.13059255246292	24.296429557093354
24	22.79649599820098	25.2985658942462	32.47649518046427	19.42844292708855
25	20.75031431754761	25.928264047183408	30.69505575942186	22.626365875847124
26	19.98536251290491	31.106459097831973	26.369596549151087	22.53858184011203
27	20.946489354090218	29.493269004712207	27.682472835808692	21.877768805388882
28	18.729686909057456	28.278275802148606	33.009782175384075	19.98225511340986
29	19.187006163690445	25.66646563972565	32.14494383170979	23.00158436487412
30	21.508683341681063	28.38143328801709	28.87289305026014	21.236990320041706
31	27.018102646400425	27.059112142367965	24.440760086271222	21.48202512496039
32	27.50968506914986	27.616195275526163	24.586766975702997	20.28735267962098
33	26.401406507139864	26.8198423812493	24.133781725628893	22.64496938598195
34	20.51975345238217	28.058795269393137	27.77405934724167	23.647391930983023
35	21.508438020668294	26.66786601384019	27.993744314174446	23.829951651317067
36	27.459067166848953	25.498379859144855	25.07798141693328	21.96457155707291
37	21.876828408173278	29.294886079054695	26.96535862865554	21.862926884116487
38	22.532980343653854	29.649661150351115	24.166164099314123	23.651194406680908
39	21.842974108411443	29.024746757162863	24.99845652196134	24.13382261246435
40	23.228547188518977	26.23385225542006	25.319622614508692	25.217977941552267
41	19.723318784434383	24.712085126391433	26.573580971266775	28.99101511790741
42	23.29192178348376	26.190961965021316	24.439083726017316	26.078032525477607
43	22.590549007983153	26.44850814159111	26.603346587482495	24.35759626294324
44	22.585274606208667	28.500086884525356	26.61479490141162	22.29984360785436
45	20.697365865625414	30.778260469585305	24.205946990217825	24.318426674571455
46	23.036379061851562	28.81671453833652	26.179636311598575	21.967270088213347
47	22.909916079770216	26.907667303819853	26.885015996974378	23.297400619435557
48	24.112725005366396	24.263679201888973	28.33175578293179	23.29184000981284
49	22.266643497459906	23.977062485306295	29.108973638212838	24.647320379020965
50	21.615643303247438	28.73048420234895	26.862323803293435	22.79154869111018
51	20.659300221811083	29.755435393689115	24.822847563655692	24.76241682084411
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1279.0
1	1680.0
2	2081.0
3	7991.5
4	13902.0
5	10088.0
6	6274.0
7	6726.5
8	7179.0
9	8146.5
10	9114.0
11	9615.0
12	10116.0
13	10219.5
14	10323.0
15	9907.0
16	9491.0
17	9372.0
18	9253.0
19	9259.0
20	9265.0
21	9413.5
22	9562.0
23	9764.5
24	9967.0
25	10535.0
26	12400.0
27	13697.0
28	17515.0
29	21333.0
30	23395.0
31	25457.0
32	31786.0
33	38115.0
34	42251.5
35	46388.0
36	51768.0
37	57148.0
38	71428.0
39	85708.0
40	113323.0
41	140938.0
42	160694.5
43	180451.0
44	189633.5
45	198816.0
46	196704.5
47	194593.0
48	202290.0
49	209987.0
50	213502.0
51	217017.0
52	206594.5
53	196172.0
54	176888.5
55	157605.0
56	140502.5
57	123400.0
58	114171.0
59	104942.0
60	98143.5
61	91345.0
62	81295.0
63	71245.0
64	60930.5
65	50616.0
66	42647.5
67	34679.0
68	29522.0
69	24365.0
70	20742.5
71	17120.0
72	14308.0
73	11496.0
74	8980.5
75	5255.0
76	4045.0
77	3054.5
78	2064.0
79	1517.5
80	971.0
81	632.5
82	294.0
83	222.5
84	151.0
85	132.0
86	113.0
87	75.5
88	38.0
89	37.5
90	37.0
91	41.5
92	46.0
93	27.5
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2445775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.900126421142495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87967048691047	15.896155411687236
2	6.960740643124834	2.7703923756593785
3	2.351330819435794	1.4037534169410262
4	1.156459178541618	0.9205473541547519
5	0.8040757862795872	0.800060489957167
6	0.5857899283852012	0.6994376178658507
7	0.42875939747443104	0.5972656349795843
8	0.3642492743334363	0.5798885286435838
9	0.30306816793254554	0.542798537047353
>10	4.2935627740311215	21.008478163703035
>50	1.8736394823429623	27.07536478195506
>100	0.9953210491628213	26.62849407074288
>500	0.0016665060226032367	0.24147304886925455
>1k	0.001458192769777832	0.4904493804587383
>5k	2.0831325282540458E-4	0.3454411873351928
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8333	0.3407099998977829	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4869	0.199078001860351	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.056587380278234914	0.0
2	0.0	0.0	0.0	0.19981396489865175	0.0
3	0.0	0.0	0.0	0.2971655201316556	0.0
4	0.0	0.0	0.0	0.4793163721110895	0.0
5	0.0	0.0	0.0	0.7579192689433819	0.0
6	0.0	0.0	0.0	1.342273921354172	0.0
7	0.0	0.0	0.0	1.6212039128701536	0.0
8	0.0	0.0	0.0	2.4459322709570586	0.0
9	0.0	0.0	0.0	2.8623238032934344	0.0
10	0.0	0.0	0.0	3.475503674704337	0.0
11	0.0	0.0	0.0	3.9351534789586124	0.0
12	0.0	0.0	0.0	4.324927681409778	0.0
13	0.0	0.0	0.0	4.540237756948207	0.0
14	0.0	0.0	0.0	4.654639122568511	0.0
15	0.0	0.0	0.0	4.756733550715008	0.0
16	0.0	0.0	0.0	4.938884402694442	0.0
17	0.0	0.0	0.0	5.129785037462563	0.0
18	0.0	0.0	0.0	5.422248571516186	0.0
19	0.0	0.0	0.0	5.54940662980037	0.0
20	2.0443417730576198E-4	0.0	0.0	5.677177990616471	0.0
21	2.0443417730576198E-4	0.0	0.0	5.833529249419918	0.0
22	2.0443417730576198E-4	0.0	0.0	6.0001839907595755	0.0
23	2.0443417730576198E-4	0.0	0.0	6.1841338634993	0.0
24	2.0443417730576198E-4	0.0	0.0	6.333043718248817	0.0
25	2.0443417730576198E-4	0.0	0.0	6.460201776533001	0.0
26	4.0886835461152396E-4	0.0	0.0	6.58719628747534	0.0
27	4.0886835461152396E-4	0.0	0.0	6.721509541965226	0.0
28	4.0886835461152396E-4	0.0	0.0	6.851488791896229	0.0
29	4.0886835461152396E-4	0.0	0.0	6.993447884617351	0.0
30	4.0886835461152396E-4	0.0	0.0	7.1927303206550075	0.0
31	4.0886835461152396E-4	0.0	0.0	7.340168249327923	0.0
32	4.0886835461152396E-4	0.0	0.0	7.49688748965052	0.0
33	4.0886835461152396E-4	0.0	0.0	7.63418548312907	0.0
34	4.0886835461152396E-4	0.0	0.0	7.775572160153734	0.0
35	4.0886835461152396E-4	0.0	0.0	7.945620508836667	0.0
36	4.0886835461152396E-4	0.0	0.0	8.094816571434412	0.0
37	4.4975519007267633E-4	0.0	0.0	8.25043186719956	0.0
38	4.4975519007267633E-4	0.0	0.0	8.408868354611524	0.0
39	4.4975519007267633E-4	0.0	0.0	8.622052314705973	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	35	1.2126839E-7	45.000004	1
CGTTTTT	5955	0.0	40.730476	1
GGCGATA	1145	0.0	40.480347	8
AGGGCGA	3285	0.0	39.726025	6
TACGGAC	40	3.4603545E-7	39.375004	4
TATGGGT	1190	0.0	38.94958	4
TAGGGTA	1570	0.0	38.837578	5
GCGATAA	250	0.0	38.699997	9
TTCGTAC	35	6.251179E-6	38.57143	33
GGGCGAT	6160	0.0	38.388798	7
TAGGGCG	1370	0.0	38.10219	5
TCACGGG	590	0.0	37.75424	3
ACACGAC	700	0.0	37.607143	26
ATAGGGT	1360	0.0	37.555145	4
AACGTAT	30	1.1402982E-4	37.500004	16
CGCGTAG	30	1.1402982E-4	37.500004	1
TAGGGTC	1160	0.0	37.435345	5
TAGGGAC	1605	0.0	37.42991	5
GACACGA	705	0.0	37.340427	25
ATAGGGC	1500	0.0	36.899998	4
>>END_MODULE