FastQCFastQC Report
Thu 26 May 2016
SRR1547682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547682_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2456330
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267651.089633721853334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT173200.042.1939931
CGTTAGG3150.039.2857132
AGGGCGA30750.038.1219526
TTAATCG2850.037.89473720
CGAATAT1850.037.702714
GGGCGAT64850.037.5751727
GTCGATA301.14029994E-437.4999968
GCGATAC1700.037.0588269
GGCGATT17550.036.9230778
ATAGGGC12000.036.56254
ACGTTAG800.036.56251
GTAGGGC12250.036.551024
TAGGGTA10850.036.290325
GTTTTTT209050.036.2389372
TTACGGG6350.036.141733
GACCGAT18850.036.047759
AATCGTT3000.036.022
TAAGGGA21600.035.8333364
TAACGGG5150.035.825243
TAGGGAC19000.035.763165
Produced by FastQC (version 0.11.5)