##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547682_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2456330
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512449467294704	31.0	31.0	33.0	30.0	34.0
2	31.941236723078738	31.0	31.0	34.0	30.0	34.0
3	32.05209682738069	33.0	31.0	34.0	30.0	34.0
4	35.77366029808698	37.0	35.0	37.0	35.0	37.0
5	35.683833605419466	37.0	35.0	37.0	33.0	37.0
6	35.72770108250927	37.0	35.0	37.0	33.0	37.0
7	36.06014053486299	37.0	35.0	37.0	35.0	37.0
8	36.07009237358172	37.0	35.0	37.0	35.0	37.0
9	37.82915569162124	39.0	38.0	39.0	35.0	39.0
10	37.25621394519466	39.0	37.0	39.0	34.0	39.0
11	37.04028815346472	39.0	37.0	39.0	33.0	39.0
12	36.67303293938518	39.0	35.0	39.0	33.0	39.0
13	36.57390782183176	39.0	35.0	39.0	32.0	39.0
14	37.69000052924485	40.0	36.0	41.0	33.0	41.0
15	37.78125699722757	40.0	36.0	41.0	33.0	41.0
16	37.80082195796167	40.0	36.0	41.0	33.0	41.0
17	37.707507134627676	40.0	36.0	41.0	33.0	41.0
18	37.54489991165682	39.0	36.0	41.0	33.0	41.0
19	37.44048234561317	39.0	36.0	41.0	32.0	41.0
20	37.29199211832287	39.0	35.0	41.0	32.0	41.0
21	37.209517450831115	39.0	35.0	41.0	32.0	41.0
22	37.109949396050204	39.0	35.0	41.0	32.0	41.0
23	36.99424629426828	39.0	35.0	41.0	32.0	41.0
24	36.863780518089996	38.0	35.0	41.0	31.0	41.0
25	36.74429575830609	38.0	35.0	41.0	31.0	41.0
26	36.59682127401449	38.0	35.0	40.0	31.0	41.0
27	36.578783795336946	38.0	35.0	41.0	31.0	41.0
28	36.559757443014576	38.0	35.0	41.0	31.0	41.0
29	36.51618023636889	38.0	35.0	41.0	31.0	41.0
30	36.4901417969084	38.0	35.0	41.0	31.0	41.0
31	36.34990697504	38.0	35.0	41.0	30.0	41.0
32	36.08469749585764	38.0	35.0	41.0	30.0	41.0
33	35.83156456990714	38.0	35.0	41.0	29.0	41.0
34	35.49700040304031	38.0	35.0	41.0	27.0	41.0
35	35.2736204011676	38.0	34.0	40.0	25.0	41.0
36	35.1101737144439	38.0	34.0	40.0	25.0	41.0
37	34.878793973122505	38.0	34.0	40.0	24.0	41.0
38	34.916700524766625	38.0	34.0	40.0	24.0	41.0
39	34.774119112659946	38.0	34.0	40.0	23.0	41.0
40	34.6268371920711	37.0	34.0	40.0	23.0	41.0
41	34.53585755985556	37.0	34.0	40.0	23.0	41.0
42	34.479208005439006	37.0	34.0	40.0	23.0	41.0
43	34.404604430186495	37.0	33.0	40.0	23.0	41.0
44	34.24222518961214	37.0	33.0	40.0	23.0	41.0
45	34.16781377095097	36.0	33.0	40.0	23.0	41.0
46	33.98477932525353	36.0	33.0	40.0	23.0	41.0
47	33.9906250381667	36.0	33.0	40.0	23.0	41.0
48	33.82347811572549	36.0	33.0	40.0	22.0	41.0
49	33.777102425162745	36.0	33.0	40.0	22.0	41.0
50	33.65848603404266	35.0	33.0	40.0	22.0	41.0
51	32.97698761974165	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	10.0
13	12.0
14	22.0
15	74.0
16	201.0
17	462.0
18	1051.0
19	2017.0
20	3734.0
21	5892.0
22	9223.0
23	13678.0
24	20167.0
25	30899.0
26	44022.0
27	51816.0
28	53186.0
29	54644.0
30	60119.0
31	70377.0
32	85745.0
33	110580.0
34	177203.0
35	245081.0
36	205900.0
37	260673.0
38	369808.0
39	579420.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.863918935973583	22.897534126114977	27.20912906653422	18.02941787137722
2	34.78620543656593	25.027785354573695	27.09131102091331	13.09469818794706
3	27.78283048287486	25.172717020921453	33.48906702275345	13.55538547345023
4	25.013943566214635	27.429132079158748	32.99080335296967	14.566121001656942
5	22.483583231894738	31.098020217153234	31.963131989594235	14.455264561357797
6	21.55606127841129	39.07182666824083	29.147386548224386	10.224725505123496
7	85.30376618776792	3.4685486070682683	9.655054491863877	1.572630713299923
8	85.80357688095654	2.954448302956036	9.481665737095586	1.7603090789918292
9	80.57492275060761	4.571494872431636	11.58630965709005	3.2672727198707014
10	41.35669881489865	24.625315002463026	20.14375104322302	13.874235139415307
11	33.22314184169065	24.71496093765903	25.28678964145697	16.77510757919335
12	28.62905228531997	22.245870872399067	30.222893503723036	18.90218333855793
13	23.915231259643452	25.52234431041432	31.334470531239695	19.227953898702534
14	21.564407062568954	26.98196089287677	31.903408743939128	19.550223300615148
15	21.38613297073276	22.424592786799817	36.80209906649351	19.387175175973912
16	23.798919526285147	21.20110082928597	34.92132571763566	20.078653926793226
17	24.07445253691483	21.642165344233064	30.632040483159834	23.651341635692273
18	25.856989899565612	21.82854095337353	31.760309078991828	20.55416006806903
19	27.460764636673414	23.82037429824169	28.032186229049028	20.686674836035877
20	28.82955466081512	23.377314937325195	27.666111638094232	20.127018763765456
21	27.062609665639386	24.54303778401111	29.006566707242104	19.387785843107398
22	26.300822772184517	22.310194477126444	28.973916371171626	22.41506637951741
23	24.890995916672434	24.62523358017856	28.550439069668975	21.93333143348003
24	24.059755814568888	23.18157576547125	32.37781568437465	20.380852735585204
25	23.906681919774623	24.215272377897108	30.14969486998897	21.728350832339302
26	22.280271787585544	25.549457931141173	29.28621154323727	22.884058738036014
27	22.293991442517903	24.44805868918264	30.12828080917466	23.12966905912479
28	21.608700785317932	25.192258369193066	32.099025782366375	21.100015063122626
29	23.049712375780125	23.377559204178592	31.541568111776513	22.03116030826477
30	23.594915992558	24.098797799969873	31.280609690066076	21.02567651740605
31	26.09515008162584	24.40840603664817	28.144182581330686	21.352261300395305
32	26.98012889147631	23.748641265627988	28.258540179861825	21.012689663033875
33	25.90116148888789	24.72579010149288	27.277727341196012	22.095321068423214
34	23.558072408837575	25.763557828141987	29.281000517031508	21.397369245988934
35	24.026820500502783	25.81558666791514	28.905847341358857	21.25174549022322
36	26.37646407445254	26.776288202318092	26.054316805966625	20.792930917262744
37	24.47956911327061	27.494514173584168	26.894757626214723	21.131159086930502
38	25.0925160707234	27.513078454442198	26.328221370907002	21.066184103927405
39	24.62869402726832	27.21462507073561	25.867574796546066	22.289106105450003
40	25.36145387631141	25.903156334857286	26.779097271132134	21.95629251769917
41	22.64777126851848	25.565660965749714	27.457059922730252	24.32950784300155
42	24.151030195454194	26.277006753978497	25.79555678593674	23.776406264630566
43	23.63314375511434	25.43603668888138	27.359760292794533	23.571059263209747
44	23.69803731583297	26.077969979603715	27.476519848717395	22.747472855845917
45	22.60758937113499	27.05520023775307	26.740258841442316	23.59695154966963
46	23.612055383437895	26.571429734604063	27.412196243990017	22.404318637968025
47	23.45743446523879	26.381552967231602	28.244779813787236	21.91623275374237
48	23.578020868531507	25.31435108474838	28.67175827352188	22.435869773198228
49	22.987912861871166	25.3169973089935	28.85630188126188	22.838787947873453
50	22.731595510375236	26.916538087309117	28.412265452931813	21.939600949383838
51	22.658641143494563	27.271457825292206	27.057317217149162	23.012583814064072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1398.0
1	2232.5
2	3067.0
3	23857.0
4	44647.0
5	30886.5
6	17126.0
7	17230.5
8	17335.0
9	18022.5
10	18710.0
11	18759.0
12	18808.0
13	18687.5
14	18567.0
15	17508.0
16	16449.0
17	16043.5
18	15638.0
19	15089.5
20	14541.0
21	14151.0
22	13761.0
23	13711.5
24	13662.0
25	14535.5
26	17082.0
27	18755.0
28	20722.5
29	22690.0
30	26552.0
31	30414.0
32	35193.5
33	39973.0
34	47093.0
35	54213.0
36	59348.5
37	64484.0
38	72081.5
39	79679.0
40	89288.0
41	98897.0
42	109810.5
43	120724.0
44	127889.0
45	135054.0
46	140216.5
47	145379.0
48	149704.0
49	154029.0
50	162497.5
51	170966.0
52	168487.0
53	166008.0
54	161361.0
55	156714.0
56	149588.5
57	142463.0
58	135347.5
59	128232.0
60	124499.0
61	120766.0
62	112123.5
63	103481.0
64	93428.0
65	83375.0
66	72619.0
67	61863.0
68	53396.0
69	44929.0
70	37368.0
71	29807.0
72	25503.5
73	21200.0
74	18095.5
75	11908.5
76	8826.0
77	7104.5
78	5383.0
79	3766.0
80	2149.0
81	1585.0
82	1021.0
83	703.0
84	385.0
85	310.0
86	235.0
87	158.5
88	82.0
89	54.0
90	26.0
91	18.5
92	11.0
93	8.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2456330.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.490292689082448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07645074601808	18.386442528274653
2	5.659531267339524	2.7720715444031736
3	2.493412477579183	1.8319320411157327
4	1.6604278223776667	1.6265745343649944
5	1.237254159723819	1.5150358251210543
6	1.0053676773936486	1.4773049207708084
7	0.8638828218406167	1.4809720209163022
8	0.7502045284115441	1.4698182781979026
9	0.6723525974966962	1.4819500712663038
>10	9.893636706303093	55.05874558945938
>50	0.6454908740407858	9.852964985307313
>100	0.04030909832179829	1.6178457432354933
>500	0.00117545620745962	0.1888033439925652
>1k	3.358446307027486E-4	0.13883687417096496
>5k	0.0	0.0
>10k+	1.679223153513743E-4	1.1007016994033998
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26765	1.089633721853334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.071114223251762E-5	0.0	0.0	0.02084410482304902	0.0
2	4.071114223251762E-5	0.0	0.0	0.07474565713890234	0.0
3	4.071114223251762E-5	0.0	0.0	0.13507956992749345	0.0
4	4.071114223251762E-5	0.0	0.0	0.2026193548912402	0.0
5	4.071114223251762E-5	0.0	0.0	0.3127022834879678	0.0
6	4.071114223251762E-5	0.0	0.0	0.6191757622143604	0.0
7	4.071114223251762E-5	0.0	0.0	0.7615833377437071	0.0
8	4.071114223251762E-5	0.0	0.0	1.1082387138535945	0.0
9	4.071114223251762E-5	0.0	0.0	1.34021080229448	0.0
10	4.071114223251762E-5	0.0	0.0	1.6757927477171226	0.0
11	4.071114223251762E-5	0.0	0.0	1.9092304372783788	0.0
12	4.071114223251762E-5	0.0	0.0	2.1146588609836625	0.0
13	4.071114223251762E-5	0.0	0.0	2.245423049834509	0.0
14	4.071114223251762E-5	0.0	0.0	2.332382049643167	0.0
15	4.071114223251762E-5	0.0	0.0	2.3999625457491462	0.0
16	4.071114223251762E-5	0.0	0.0	2.4840717656015276	0.0
17	4.071114223251762E-5	0.0	0.0	2.5605680018564283	0.0
18	4.071114223251762E-5	0.0	0.0	2.672930754418177	0.0
19	4.071114223251762E-5	0.0	0.0	2.7395341831105755	0.0
20	8.142228446503523E-5	0.0	0.0	2.8139541511116177	0.0
21	8.142228446503523E-5	0.0	0.0	2.886745673423359	0.0
22	1.2213342669755285E-4	0.0	0.0	2.967720135323837	0.0
23	1.2213342669755285E-4	0.0	0.0	3.0545977128480293	0.0
24	1.2213342669755285E-4	0.0	0.0	3.134310129339299	0.0
25	1.2213342669755285E-4	0.0	0.0	3.206979518224343	0.0
26	1.2213342669755285E-4	0.0	0.0	3.2796489071093866	0.0
27	1.2213342669755285E-4	0.0	0.0	3.36200754784577	0.0
28	1.2213342669755285E-4	0.0	0.0	3.4381373838205778	0.0
29	1.2213342669755285E-4	0.0	0.0	3.522043047961797	0.0
30	1.2213342669755285E-4	0.0	0.0	3.632248109985222	0.0
31	1.2213342669755285E-4	0.0	0.0	3.7174972418201135	0.0
32	1.2213342669755285E-4	0.0	0.0	3.82399758990038	0.0
33	1.2213342669755285E-4	0.0	0.0	3.9107123228556424	0.0
34	1.2213342669755285E-4	0.0	0.0	3.9975899003798347	0.0
35	1.2213342669755285E-4	0.0	0.0	4.0883757475583495	0.0
36	1.2213342669755285E-4	0.0	0.0	4.17716674876747	0.0
37	1.2213342669755285E-4	0.0	0.0	4.270720953617795	0.0
38	1.2213342669755285E-4	0.0	0.0	4.383002283895079	0.0
39	1.2213342669755285E-4	0.0	0.0	4.54083938233055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17320	0.0	42.193993	1
CGTTAGG	315	0.0	39.285713	2
AGGGCGA	3075	0.0	38.121952	6
TTAATCG	285	0.0	37.894737	20
CGAATAT	185	0.0	37.7027	14
GGGCGAT	6485	0.0	37.575172	7
GTCGATA	30	1.14029994E-4	37.499996	8
GCGATAC	170	0.0	37.058826	9
GGCGATT	1755	0.0	36.923077	8
ATAGGGC	1200	0.0	36.5625	4
ACGTTAG	80	0.0	36.5625	1
GTAGGGC	1225	0.0	36.55102	4
TAGGGTA	1085	0.0	36.29032	5
GTTTTTT	20905	0.0	36.238937	2
TTACGGG	635	0.0	36.14173	3
GACCGAT	1885	0.0	36.04775	9
AATCGTT	300	0.0	36.0	22
TAAGGGA	2160	0.0	35.833336	4
TAACGGG	515	0.0	35.82524	3
TAGGGAC	1900	0.0	35.76316	5
>>END_MODULE