FastQCFastQC Report
Thu 26 May 2016
SRR1547681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547681_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8164393
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT567700.6953364444852177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT384250.041.691611
GGGCGAT221950.038.1876567
AGGGCGA112450.038.1769686
CACGACC72250.036.9965427
TAACGGG23650.036.7230453
GCGAGAC73150.036.69514521
ACACGAC72800.036.6552226
CGAGACA73950.036.51115822
AACACGT67300.036.27414741
GACACGA73450.036.26957325
ACGACCA73850.036.22545628
GACCGAT58850.036.206469
CGACCAA74150.036.16992629
TAGGGCG45550.036.158075
ACGTAGG7300.036.0616462
TGGGCGA72000.035.906256
GCGATAT12050.035.8506249
GGCGATA42850.035.5484248
GGCGATT58200.035.528358
GTCAAGC75550.035.44010516
Produced by FastQC (version 0.11.5)