##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547681_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	8164393
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375227037698945	31.0	31.0	33.0	30.0	34.0
2	31.782475316903536	31.0	31.0	34.0	30.0	34.0
3	31.911166451688448	31.0	31.0	34.0	30.0	34.0
4	35.623561114708714	37.0	35.0	37.0	33.0	37.0
5	35.51231928693291	37.0	35.0	37.0	33.0	37.0
6	35.55605701489382	37.0	35.0	37.0	33.0	37.0
7	35.97784624037574	37.0	35.0	37.0	35.0	37.0
8	35.98648141999044	37.0	35.0	37.0	35.0	37.0
9	37.72105103220778	39.0	37.0	39.0	35.0	39.0
10	37.06928377896556	39.0	37.0	39.0	33.0	39.0
11	36.85168891306433	39.0	37.0	39.0	32.0	39.0
12	36.42491254401889	39.0	35.0	39.0	32.0	39.0
13	36.27786180797519	39.0	35.0	39.0	32.0	39.0
14	37.300159485218316	39.0	35.0	41.0	32.0	41.0
15	37.40243542906374	39.0	35.0	41.0	32.0	41.0
16	37.4402322377181	39.0	35.0	41.0	32.0	41.0
17	37.339198639751906	39.0	35.0	41.0	32.0	41.0
18	37.22471088787617	39.0	35.0	41.0	32.0	41.0
19	37.1716503602901	39.0	35.0	41.0	32.0	41.0
20	37.07758947909539	39.0	35.0	41.0	32.0	41.0
21	36.97735177618226	39.0	35.0	41.0	31.0	41.0
22	36.88614426081645	39.0	35.0	41.0	31.0	41.0
23	36.768697072764624	38.0	35.0	41.0	31.0	41.0
24	36.65720782916746	38.0	35.0	41.0	31.0	41.0
25	36.533218207403785	38.0	35.0	40.0	31.0	41.0
26	36.365968786656886	38.0	35.0	40.0	30.0	41.0
27	36.34879408671288	38.0	35.0	40.0	30.0	41.0
28	36.35018279987257	38.0	35.0	40.0	30.0	41.0
29	36.324405868262346	38.0	35.0	40.0	30.0	41.0
30	36.3289428865073	38.0	35.0	40.0	30.0	41.0
31	36.20331774817797	38.0	35.0	40.0	30.0	41.0
32	35.99098242820991	38.0	35.0	40.0	30.0	41.0
33	35.851008887004824	38.0	35.0	40.0	29.0	41.0
34	35.60294770229704	38.0	35.0	40.0	27.0	41.0
35	35.46072537174533	38.0	34.0	40.0	27.0	41.0
36	35.341931972162534	38.0	34.0	40.0	26.0	41.0
37	35.10298120141938	38.0	34.0	40.0	25.0	41.0
38	35.15987912389813	38.0	34.0	40.0	26.0	41.0
39	35.04696405476806	38.0	34.0	40.0	25.0	41.0
40	34.90171896428797	38.0	34.0	40.0	24.0	41.0
41	34.824592593717625	37.0	34.0	40.0	24.0	41.0
42	34.787810679863156	37.0	34.0	40.0	24.0	41.0
43	34.70010814031123	37.0	34.0	40.0	24.0	41.0
44	34.53700170974131	37.0	33.0	40.0	23.0	41.0
45	34.45356562331088	37.0	33.0	40.0	23.0	41.0
46	34.24393705692511	36.0	33.0	40.0	23.0	41.0
47	34.2577166483779	36.0	33.0	40.0	23.0	41.0
48	34.081202729951876	36.0	33.0	40.0	23.0	41.0
49	34.05000984151547	36.0	33.0	40.0	23.0	41.0
50	33.9032809910057	35.0	33.0	40.0	23.0	41.0
51	33.22020130584111	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	8.0
12	15.0
13	43.0
14	102.0
15	270.0
16	754.0
17	1950.0
18	4238.0
19	8154.0
20	13737.0
21	21786.0
22	32920.0
23	47631.0
24	68638.0
25	98123.0
26	132150.0
27	156096.0
28	169228.0
29	185420.0
30	214228.0
31	253348.0
32	309310.0
33	391615.0
34	616408.0
35	827277.0
36	661304.0
37	837499.0
38	1192087.0
39	1919078.0
40	968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.91952102256714	23.711462689265446	27.189847426501885	15.179168861665527
2	32.647497493077566	24.698333360483748	28.189199123560073	14.464970022878616
3	28.912645434878016	25.513041814621122	31.657674979634127	13.916637770866739
4	25.212456088284824	28.15076393309337	30.898096159751255	15.738683818870552
5	23.092016761074582	31.67811740566629	30.135528752719274	15.094337080539852
6	21.372329822927437	40.115376611586434	27.37758949134369	11.134704074142437
7	86.87819167940593	3.6572467787868614	7.675034751511839	1.7895267902953715
8	87.2849457393832	2.8933198095682067	7.365238297568479	2.456496153480118
9	81.69312770710572	4.624875358155836	10.051953647993182	3.630043286745261
10	40.57890157908861	25.514119665724078	17.657038312584906	16.24994044260241
11	34.41938671987005	25.82690470681654	23.14828548797198	16.605423085341435
12	29.89889144238892	23.04689399444637	27.359376257365366	19.69483830579934
13	24.702338557195862	27.3128694319345	28.777754819004915	19.207037191864725
14	21.76989765191362	28.81527138637251	29.957462851188083	19.457368110525792
15	21.824929299704216	23.318414485926876	36.05601788154979	18.80063833281911
16	24.576915883397575	22.402326786571887	33.6485517049461	19.372205625084437
17	23.80158573944199	23.214095646792114	28.918524622712305	24.065793991053592
18	26.44675482917101	22.685715888492876	30.28836313979496	20.579166142541155
19	27.491706976868947	25.994596291481802	26.41746912477143	20.096227606877818
20	30.06933889635151	24.92111783447955	25.431872277583896	19.577670991585045
21	27.189271756026447	26.696412090892736	26.69898423557024	19.415331917510585
22	26.39196814754018	23.31268227778844	27.392287460929428	22.903062113741953
23	24.324221041294802	27.017819940808828	26.890413041116467	21.767545976779903
24	24.012256636837545	24.978746123563624	30.855998725196105	20.15299851440272
25	24.181075065837717	25.219694838305802	28.73309748808025	21.86613260777623
26	22.18451512561926	27.452598129462903	27.65449923833897	22.708387506578873
27	22.744164814212155	25.673898843429022	28.55272890464729	23.029207437711534
28	21.019664291025677	27.117876368763728	29.943156827457962	21.91930251275263
29	22.33567149450057	25.731992568216643	29.199317083339814	22.73301885394297
30	24.028363161841916	25.329133470179592	29.043224646339294	21.599278721639195
31	25.816983577346164	25.79721480825335	26.829404219027676	21.55639739537281
32	26.420371973764617	24.938106237659063	26.423862741541228	22.217659047035095
33	24.828851330405087	26.19775897608065	25.569261548286565	23.4041281452277
34	21.890139291432934	26.73592513246239	29.08044725431517	22.293488321789507
35	21.832449760808917	27.3151353689123	28.579503705909303	22.27291116436948
36	24.461585815381497	28.115929745175176	26.167383172270124	21.2551012671732
37	23.22378405841071	28.05443099076686	26.387264797272742	22.33452015354969
38	23.296024578924605	28.376206289922596	26.733695940408552	21.594073190744247
39	23.228744623145896	26.95742353411944	26.306854655330774	23.506977187403887
40	25.14365734231559	25.312843220555404	26.767966706159296	22.77553273096971
41	21.222863720548485	25.8426070376573	26.501076075098297	26.43345316669592
42	23.612141159789836	26.50798412080359	25.646548371691562	24.23332634771501
43	22.912579049048716	25.535554204703274	26.840978870076444	24.710887876171565
44	23.091098137975475	26.595816737386357	26.817364132275358	23.495720992362813
45	22.198747659501446	26.864275151869833	26.286375484374652	24.65060170425407
46	23.57678029462815	26.686552202962304	26.21085241731994	23.525815085089608
47	22.736154420787926	26.627307137223795	27.92791821755763	22.708620224430646
48	23.326032933495487	25.670432572268385	28.08446384195371	22.919070652282418
49	23.634678046487963	24.859190389291648	27.883481356176752	23.622650208043634
50	22.011740493138927	27.09642958147654	27.867289093016467	23.024540832368064
51	21.88775087137525	26.837990282927343	26.78695158354087	24.487307262156538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4165.0
1	5935.0
2	7705.0
3	51066.0
4	94427.0
5	65198.0
6	35969.0
7	36663.5
8	37358.0
9	39447.5
10	41537.0
11	42353.0
12	43169.0
13	43481.0
14	43793.0
15	42749.0
16	41705.0
17	40272.5
18	38840.0
19	38074.5
20	37309.0
21	37343.5
22	37378.0
23	36707.0
24	36036.0
25	39452.0
26	50539.0
27	58210.0
28	65866.5
29	73523.0
30	86169.0
31	98815.0
32	108970.0
33	119125.0
34	142928.5
35	166732.0
36	185196.0
37	203660.0
38	231925.5
39	260191.0
40	307275.0
41	354359.0
42	408063.5
43	461768.0
44	488145.5
45	514523.0
46	557627.0
47	600731.0
48	623085.5
49	645440.0
50	651370.0
51	657300.0
52	635941.0
53	614582.0
54	575091.0
55	535600.0
56	509555.5
57	483511.0
58	456600.5
59	429690.0
60	397646.0
61	365602.0
62	331252.5
63	296903.0
64	259811.5
65	222720.0
66	188529.0
67	154338.0
68	127974.5
69	101611.0
70	85719.5
71	69828.0
72	60408.0
73	50988.0
74	44988.0
75	30936.0
76	22884.0
77	17055.0
78	11226.0
79	8206.0
80	5186.0
81	3728.0
82	2270.0
83	1617.0
84	964.0
85	745.0
86	526.0
87	348.0
88	170.0
89	136.0
90	102.0
91	74.5
92	47.0
93	31.5
94	16.0
95	8.5
96	1.0
97	2.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8164393.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.09086761310167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55213840272862	15.378102101987345
2	7.35052320821155	2.8065573090999605
3	2.4495707340779624	1.4029329177963195
4	1.2422747582636864	0.9486441179643965
5	0.7577659633151828	0.7233204843682304
6	0.5442415121707475	0.6234025595043599
7	0.42232201238283296	0.5643745539949542
8	0.3392341548912907	0.5181019472697646
9	0.2767335427558759	0.47547750859713345
>10	3.3310764555924584	15.419012830152942
>50	1.4040158161112826	19.38445162187699
>100	1.3214789071041055	39.287336021586675
>500	0.005684354002657057	0.7349245491530418
>1k	0.0028095037967153487	0.9488433994955436
>5k	6.533729759732528E-5	0.07639992404098721
>10k+	6.533729759732528E-5	0.7081181531113214
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56770	0.6953364444852177	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.573815591679626E-5	0.0	0.0	0.049740378739729946	0.0
2	8.573815591679626E-5	0.0	0.0	0.16652799540639457	0.0
3	8.573815591679626E-5	0.0	0.0	0.2640735202237325	0.0
4	8.573815591679626E-5	0.0	0.0	0.4297196374549829	0.0
5	8.573815591679626E-5	0.0	0.0	0.7269738240185155	0.0
6	8.573815591679626E-5	0.0	0.0	1.4114460193182763	0.0
7	8.573815591679626E-5	0.0	0.0	1.7820063291906698	0.0
8	8.573815591679626E-5	0.0	0.0	2.6581400478884345	0.0
9	8.573815591679626E-5	0.0	0.0	3.1324557747281396	0.0
10	1.224830798811375E-4	0.0	0.0	3.7888425998111557	0.0
11	1.224830798811375E-4	0.0	0.0	4.1982912875458105	0.0
12	1.224830798811375E-4	0.0	0.0	4.546486187031908	0.0
13	1.224830798811375E-4	0.0	0.0	4.755381079769188	0.0
14	1.224830798811375E-4	0.0	0.0	4.876541342387609	0.0
15	1.224830798811375E-4	0.0	0.0	4.982097260629174	0.0
16	1.224830798811375E-4	0.0	0.0	5.132286013179424	0.0
17	1.224830798811375E-4	0.0	0.0	5.28581855381043	0.0
18	1.224830798811375E-4	0.0	0.0	5.517850500337257	0.0
19	1.224830798811375E-4	0.0	0.0	5.638484085712189	0.0
20	2.2046954378604754E-4	0.0	0.0	5.7616285742246856	0.0
21	2.3271785177416128E-4	0.0	0.0	5.90192069392054	0.0
22	3.184560076909576E-4	0.0	0.0	6.050406931660443	0.0
23	3.184560076909576E-4	0.0	0.0	6.221283076402618	0.0
24	3.184560076909576E-4	0.0	0.0	6.371018641557309	0.0
25	3.184560076909576E-4	0.0	0.0	6.500005572980134	0.0
26	3.184560076909576E-4	0.0	0.0	6.62912723579083	0.0
27	3.184560076909576E-4	0.0	0.0	6.766883955733145	0.0
28	3.184560076909576E-4	0.0	0.0	6.9067841295733805	0.0
29	3.184560076909576E-4	0.0	0.0	7.06094133391178	0.0
30	3.184560076909576E-4	0.0	0.0	7.264581702522159	0.0
31	3.307043156790713E-4	0.0	0.0	7.4196697782676555	0.0
32	3.307043156790713E-4	0.0	0.0	7.6092858342316445	0.0
33	3.307043156790713E-4	0.0	0.0	7.773021215416749	0.0
34	3.307043156790713E-4	0.0	0.0	7.932065494642407	0.0
35	3.307043156790713E-4	0.0	0.0	8.100969661798494	0.0
36	3.307043156790713E-4	0.0	0.0	8.258286929597828	0.0
37	3.307043156790713E-4	0.0	0.0	8.423087913577898	0.0
38	3.307043156790713E-4	0.0	0.0	8.621816710685044	0.0
39	3.307043156790713E-4	0.0	0.0	8.923296563504476	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	38425	0.0	41.69161	1
GGGCGAT	22195	0.0	38.187656	7
AGGGCGA	11245	0.0	38.176968	6
CACGACC	7225	0.0	36.99654	27
TAACGGG	2365	0.0	36.723045	3
GCGAGAC	7315	0.0	36.695145	21
ACACGAC	7280	0.0	36.65522	26
CGAGACA	7395	0.0	36.511158	22
AACACGT	6730	0.0	36.274147	41
GACACGA	7345	0.0	36.269573	25
ACGACCA	7385	0.0	36.225456	28
GACCGAT	5885	0.0	36.20646	9
CGACCAA	7415	0.0	36.169926	29
TAGGGCG	4555	0.0	36.15807	5
ACGTAGG	730	0.0	36.061646	2
TGGGCGA	7200	0.0	35.90625	6
GCGATAT	1205	0.0	35.850624	9
GGCGATA	4285	0.0	35.548424	8
GGCGATT	5820	0.0	35.52835	8
GTCAAGC	7555	0.0	35.440105	16
>>END_MODULE