FastQCFastQC Report
Thu 26 May 2016
SRR1547680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547680_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7437348
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497640.6691094728927569No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC85310.11470486522884232No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT394850.042.276181
CGTTAGG11550.039.5454522
AGGGCGA92500.039.478386
GGGCGAT176900.038.360667
TATAGCG3950.037.5949361
TACGGGA16550.037.1148034
CGTAAGG14150.036.5724372
TATCGCG2400.036.5625041
TACGGGT10850.036.4976964
GCGTTAG6250.036.01
TAGGGCG35350.035.834515
GGCGATA40750.035.7239278
GGCGATT45500.035.6043938
GTAGGGC45100.035.3215074
TGGGCGA57100.035.148866
ACGGGTA12100.035.144635
GTTTTTT496350.035.0498662
ATAGGGC43450.034.9021874
TAGCGGG42950.034.784633
AAGGGCG41450.034.740655
Produced by FastQC (version 0.11.5)