FastQCFastQC Report
Thu 26 May 2016
SRR1547679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547679_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2211921
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91330.4128990140244611No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC80890.36570022166252775No Hit
GAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT24330.10999488679749411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCATA302.1664164E-645.00000429
GTTACCC453.8562575E-1045.00000426
ACTAGTC600.045.00000410
CCGTAGA453.8562575E-1045.00000433
CCATCCG302.1664164E-645.00000445
CTAGTCG600.045.0000041
TACGACA302.1664164E-645.00000437
GTACTCC302.1664164E-645.00000442
ATCGGAC453.8562575E-1045.00000438
ACGCGAT207.0347043E-445.013
CGGCGTA207.0347043E-445.024
GGTACGA207.0347043E-445.021
CGAACTT207.0347043E-445.034
CGAGTTA253.89198E-545.025
TCCGATT207.0347043E-445.024
ACGTTAG406.8193913E-945.01
GCGAACT207.0347043E-445.033
TTCGTTA351.2126111E-745.015
ATTCGTC253.89198E-545.014
CGTTCCC207.0347043E-445.018