##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547679_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2211921
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.511402984102958	31.0	31.0	33.0	30.0	34.0
2	31.90731133706855	31.0	31.0	34.0	30.0	34.0
3	32.02738253310132	33.0	31.0	34.0	30.0	34.0
4	35.68987047909939	37.0	35.0	37.0	33.0	37.0
5	35.576354218798954	37.0	35.0	37.0	33.0	37.0
6	35.624980729420265	37.0	35.0	37.0	33.0	37.0
7	36.02376531530737	37.0	35.0	37.0	35.0	37.0
8	36.055135784686705	37.0	35.0	37.0	35.0	37.0
9	37.82567822268517	39.0	38.0	39.0	35.0	39.0
10	37.28923727384477	39.0	37.0	39.0	34.0	39.0
11	36.948314157693694	39.0	37.0	39.0	33.0	39.0
12	35.76089516759414	37.0	35.0	39.0	32.0	39.0
13	35.19495045257041	37.0	35.0	39.0	30.0	39.0
14	36.11982706434814	38.0	35.0	41.0	31.0	41.0
15	36.525139912320554	38.0	35.0	41.0	32.0	41.0
16	36.73681022061819	38.0	35.0	41.0	32.0	41.0
17	36.69584582812858	38.0	35.0	40.0	32.0	41.0
18	36.634254568766245	37.0	35.0	40.0	32.0	41.0
19	36.56013438092952	37.0	35.0	40.0	32.0	41.0
20	36.417787977057046	36.0	35.0	40.0	32.0	41.0
21	36.237149970545964	36.0	35.0	40.0	31.0	41.0
22	36.123043273245294	36.0	35.0	40.0	31.0	41.0
23	36.09492020736726	36.0	35.0	40.0	31.0	41.0
24	35.97514649031317	35.0	35.0	40.0	31.0	41.0
25	35.85296671987833	35.0	35.0	40.0	31.0	41.0
26	35.68186341193921	35.0	34.0	40.0	31.0	41.0
27	35.61189979208118	35.0	34.0	40.0	30.0	41.0
28	35.71994117330592	36.0	35.0	40.0	31.0	41.0
29	35.746889242427734	36.0	35.0	40.0	31.0	41.0
30	35.782869731785176	36.0	35.0	40.0	31.0	41.0
31	35.54801866793615	35.0	35.0	40.0	30.0	41.0
32	35.281711688618174	35.0	34.0	40.0	30.0	41.0
33	35.15062789313	35.0	34.0	40.0	29.0	41.0
34	34.996679809089024	35.0	34.0	40.0	29.0	41.0
35	34.86914270446368	35.0	34.0	40.0	29.0	41.0
36	34.63450231721657	35.0	34.0	40.0	26.0	41.0
37	34.33840494303368	35.0	34.0	40.0	25.0	41.0
38	34.42148973674919	35.0	33.0	40.0	26.0	41.0
39	34.348705491742244	35.0	34.0	40.0	25.0	41.0
40	34.15283366811021	35.0	33.0	40.0	24.0	41.0
41	34.154793050927225	35.0	34.0	40.0	24.0	41.0
42	34.118567073598015	35.0	34.0	40.0	24.0	41.0
43	34.01977331016795	35.0	33.0	40.0	24.0	41.0
44	33.87677769685264	35.0	33.0	40.0	23.0	41.0
45	33.81689581137844	35.0	33.0	39.0	23.0	41.0
46	33.70411511080188	35.0	33.0	39.0	23.0	41.0
47	33.71550927903845	35.0	33.0	39.0	23.0	41.0
48	33.606895544641965	35.0	33.0	39.0	23.0	41.0
49	33.63281735649691	35.0	33.0	39.0	24.0	41.0
50	33.458903821610264	35.0	33.0	39.0	24.0	41.0
51	32.72771902793997	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	4.0
14	29.0
15	79.0
16	202.0
17	507.0
18	1201.0
19	2338.0
20	4056.0
21	6408.0
22	9723.0
23	14111.0
24	20645.0
25	28358.0
26	35400.0
27	40785.0
28	45150.0
29	50908.0
30	59577.0
31	72251.0
32	90782.0
33	121101.0
34	232198.0
35	431548.0
36	143123.0
37	160208.0
38	229473.0
39	411415.0
40	333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.12831742182474	25.882027432263634	28.523577469538917	13.46607767637271
2	28.67466785658258	26.93929846499943	30.771759027560208	13.614274650857784
3	28.449433772725158	25.87190048830858	31.85592975517661	13.822735983789656
4	23.46991596896996	29.866889459433676	31.577212748556573	15.085981823039793
5	22.7726939614932	33.10538667520223	30.197235796395983	13.924683566908582
6	20.499285462726743	40.53847311906709	27.569791145343796	11.392450272862368
7	86.68451540538744	5.407019509286272	6.2464708278460215	1.6619942574802629
8	87.56962839088737	3.9925024447075645	5.462808120181507	2.9750610442235503
9	82.83632191203935	5.350959640963669	8.556951175019362	3.2557672719776156
10	57.251818668026566	19.56774224757575	11.857475922512602	11.322963161885076
11	51.97608775358613	19.70545060153595	17.592626499771015	10.7258351451069
12	45.008976360367306	21.11770718755326	20.38255434981629	13.490762102263146
13	20.508869891827057	45.30505384233886	22.452067682344897	11.734008583489192
14	14.44215231918319	47.0655145459535	25.804176550609174	12.68815658425414
15	12.113452514805005	23.519375239893286	52.42642029258731	11.940751952714406
16	13.915325185664408	17.73707107984417	50.889973014406934	17.457630720084488
17	14.127222446009599	19.13513186049592	27.637108196902147	39.10053749659233
18	20.89156891227128	23.151369330098138	34.78930757472803	21.167754182902556
19	27.972472796270754	25.7154301622888	24.973360260153957	21.338736781286492
20	30.925471569735087	24.65684805198739	22.69651583397418	21.721164544303345
21	20.81516473689612	30.566281526329377	26.877632609844564	21.74092112692994
22	23.085318146534167	25.954227117514595	24.198468209307656	26.761986526643582
23	18.450161646821925	33.64419434509641	22.08279590455536	25.8228481035263
24	20.058672981539576	24.82846358436852	37.821965612695934	17.290897821395973
25	15.593097583503207	25.670808315486855	36.392258132184644	22.34383596882529
26	15.213744071329854	36.52472217588241	27.298940604117416	20.96259314867032
27	17.873332727525078	34.738265968811724	27.198620565562692	20.189780738100502
28	14.018086541065436	29.6704086628772	39.80824812459396	16.5032566714634
29	13.855829389928484	24.6959091215283	39.34584463007494	22.102416858468274
30	17.48046155355458	30.13620287523831	32.76694782499013	19.616387746216976
31	29.537854200036982	26.562476688814833	23.788326979128097	20.111342132020084
32	29.791389475483076	27.024156830194208	26.040713027273576	17.14374066704914
33	27.039302036555558	27.676304895156743	24.355255002326032	20.92913806596167
34	18.667664893999376	27.755286016091894	29.66588770575441	23.911161384154315
35	19.238526149894142	25.80756726845127	31.21892689657542	23.734979685079168
36	30.55045817639961	23.168684595878425	26.647109006153475	19.633748221568492
37	20.413748953963545	30.711132992543583	29.297158442819608	19.577959610673258
38	20.91222968632243	33.73908923510378	22.17475217243292	23.173928906140862
39	19.963868510674658	31.60316304244139	26.515910830450096	21.917057616433862
40	23.139298374580285	26.743088925870317	23.65292431330052	26.464688386248874
41	16.287426178421384	24.63003877624924	26.306771353949802	32.775763691379574
42	22.94123524303083	26.97076432657405	23.290614809480083	26.797385620915033
43	22.93694033376418	26.839520941299437	25.768777456337727	24.454761268598652
44	20.25009030611853	31.112051470192654	27.187092124899575	21.45076609878924
45	16.525544990078757	36.53254343170484	22.416487749788534	24.52542382842787
46	23.229310630895046	31.0291823261319	26.28913962117092	19.452367421802137
47	23.357163298327563	26.275667168945006	26.74652485328364	23.620644679443796
48	24.806808199750353	22.423133556759034	30.191584599992495	22.578473643498118
49	21.431913707587206	21.07154821532957	32.30495121661217	25.191586860471055
50	19.055020500280072	31.948609376193815	26.45655066342785	22.539819460098258
51	18.19513445552531	33.69559762758254	23.31520881622807	24.794059100664086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2468.0
1	2710.5
2	2953.0
3	8808.0
4	14663.0
5	10761.0
6	6859.0
7	7972.5
8	9086.0
9	10817.5
10	12549.0
11	13603.5
12	14658.0
13	14876.5
14	15095.0
15	15060.5
16	15026.0
17	14071.5
18	13117.0
19	12770.0
20	12423.0
21	12836.5
22	13250.0
23	12871.0
24	12492.0
25	11165.5
26	10867.5
27	11896.0
28	13576.5
29	15257.0
30	19355.0
31	23453.0
32	23753.5
33	24054.0
34	28006.5
35	31959.0
36	37750.0
37	43541.0
38	60001.0
39	76461.0
40	115421.5
41	154382.0
42	192930.0
43	231478.0
44	243657.5
45	255837.0
46	250534.5
47	245232.0
48	220119.0
49	195006.0
50	182863.0
51	170720.0
52	150925.5
53	131131.0
54	119906.5
55	108682.0
56	93141.0
57	77600.0
58	73039.0
59	68478.0
60	62997.0
61	57516.0
62	48695.0
63	39874.0
64	34680.0
65	29486.0
66	26412.0
67	23338.0
68	19609.5
69	15881.0
70	12637.5
71	9394.0
72	8102.0
73	6810.0
74	5604.0
75	3337.5
76	2277.0
77	1934.0
78	1591.0
79	1169.5
80	748.0
81	662.5
82	577.0
83	414.5
84	252.0
85	154.0
86	56.0
87	57.5
88	59.0
89	35.5
90	12.0
91	8.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2211921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.310922766299797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7393741167592	15.977736633007211
2	7.5125425309843825	2.901482571887636
3	2.395842400330228	1.3879778265901006
4	1.147099664333389	0.8860621209276198
5	0.6916353938146595	0.6678058836197116
6	0.4532738172825585	0.5251881406517624
7	0.3344158590771853	0.45205151785257247
8	0.2558844277462522	0.3953091537045357
9	0.2059177822883661	0.35788161509805366
>10	1.9564366210355424	8.554832945061412
>50	0.7611852744239863	10.866541787320978
>100	1.535551813672652	54.61812565479971
>500	0.0077767357022860565	0.8633296380489606
>1k	0.0025922452340953524	0.7619381229167544
>5k	4.71317315290064E-4	0.7837363885129514
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9133	0.4128990140244611	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8089	0.36570022166252775	No Hit
GAGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	2433	0.10999488679749411	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.056918850175933046	0.0
2	0.0	0.0	0.0	0.2122589369150164	0.0
3	0.0	0.0	0.0	0.32686519997775687	0.0
4	0.0	0.0	0.0	0.6768777004242014	0.0
5	0.0	0.0	0.0	1.1109800033545503	0.0
6	0.0	0.0	0.0	1.9750705382335083	0.0
7	0.0	0.0	0.0	2.371920154472063	0.0
8	0.0	0.0	0.0	3.5397737984313182	0.0
9	0.0	0.0	0.0	4.140111694766675	0.0
10	0.0	0.0	0.0	5.069484850498729	0.0
11	0.0	0.0	0.0	5.727419740578438	0.0
12	0.0	0.0	0.0	6.291906446930066	0.0
13	0.0	0.0	0.0	6.566418963425909	0.0
14	0.0	0.0	0.0	6.676459059794631	0.0
15	0.0	0.0	0.0	6.778813529054609	0.0
16	0.0	0.0	0.0	6.966071573080594	0.0
17	0.0	0.0	0.0	7.161648178212513	0.0
18	0.0	0.0	0.0	7.503477746266706	0.0
19	0.0	0.0	0.0	7.621836403741363	0.0
20	1.8083828491162208E-4	0.0	0.0	7.762890265972429	0.0
21	1.8083828491162208E-4	0.0	0.0	7.93120549965392	0.0
22	2.260478561395276E-4	0.0	0.0	8.09201594451158	0.0
23	2.260478561395276E-4	0.0	0.0	8.273215905993027	0.0
24	2.260478561395276E-4	0.0	0.0	8.407850009109728	0.0
25	2.260478561395276E-4	0.0	0.0	8.520105374468619	0.0
26	2.260478561395276E-4	0.0	0.0	8.633671817393116	0.0
27	2.260478561395276E-4	0.0	0.0	8.738422393928174	0.0
28	2.260478561395276E-4	0.0	0.0	8.836753211348869	0.0
29	2.260478561395276E-4	0.0	0.0	8.946476840718995	0.0
30	2.260478561395276E-4	0.0	0.0	9.124738180070626	0.0
31	2.260478561395276E-4	0.0	0.0	9.264797431734678	0.0
32	2.260478561395276E-4	0.0	0.0	9.377730940661985	0.0
33	2.260478561395276E-4	0.0	0.0	9.48315966076546	0.0
34	2.260478561395276E-4	0.0	0.0	9.593832691131373	0.0
35	2.260478561395276E-4	0.0	0.0	9.74392846760802	0.0
36	2.260478561395276E-4	0.0	0.0	9.86784790234371	0.0
37	2.260478561395276E-4	0.0	0.0	9.99149607965203	0.0
38	2.260478561395276E-4	0.0	0.0	10.105740666144948	0.0
39	2.260478561395276E-4	0.0	0.0	10.22703794574942	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCATA	30	2.1664164E-6	45.000004	29
GTTACCC	45	3.8562575E-10	45.000004	26
ACTAGTC	60	0.0	45.000004	10
CCGTAGA	45	3.8562575E-10	45.000004	33
CCATCCG	30	2.1664164E-6	45.000004	45
CTAGTCG	60	0.0	45.000004	1
TACGACA	30	2.1664164E-6	45.000004	37
GTACTCC	30	2.1664164E-6	45.000004	42
ATCGGAC	45	3.8562575E-10	45.000004	38
ACGCGAT	20	7.0347043E-4	45.0	13
CGGCGTA	20	7.0347043E-4	45.0	24
GGTACGA	20	7.0347043E-4	45.0	21
CGAACTT	20	7.0347043E-4	45.0	34
CGAGTTA	25	3.89198E-5	45.0	25
TCCGATT	20	7.0347043E-4	45.0	24
ACGTTAG	40	6.8193913E-9	45.0	1
GCGAACT	20	7.0347043E-4	45.0	33
TTCGTTA	35	1.2126111E-7	45.0	15
ATTCGTC	25	3.89198E-5	45.0	14
CGTTCCC	20	7.0347043E-4	45.0	18
>>END_MODULE