FastQCFastQC Report
Thu 26 May 2016
SRR1547678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547678_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2882464
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536911.8626772094985402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT343450.043.59151
TATTACG800.042.18751
GTTTTTT392200.038.7238662
TACGGGA7650.037.9411744
CACGACC14850.037.42424427
GCGATAT3250.037.3846179
ACACGAC14900.036.99664726
GCGATAC1950.036.9230779
GCGTTAG2200.036.8181841
TAGGGAC23750.036.663165
GGGCGAT71350.036.4856347
TATAGCG1050.036.428571
GGCGATA13500.036.1666688
GACACGA15150.036.0891125
CGGTCTA751.8189894E-1236.00000431
CGTATGG4250.036.02
AATCGTA250.002107634736.08
AGGGCGA35400.035.9110156
GCGAGAC15300.035.8823521
ACGGGTA3650.035.7534265
Produced by FastQC (version 0.11.5)