##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547678_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2882464
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.440034290107352	31.0	31.0	33.0	30.0	34.0
2	31.863927875595323	31.0	31.0	34.0	30.0	34.0
3	31.895351338299456	31.0	31.0	34.0	30.0	34.0
4	35.647596639541725	37.0	35.0	37.0	33.0	37.0
5	35.55750878415134	37.0	35.0	37.0	33.0	37.0
6	35.61336689721016	37.0	35.0	37.0	33.0	37.0
7	36.03075667206945	37.0	35.0	37.0	35.0	37.0
8	36.048437725501515	37.0	35.0	37.0	35.0	37.0
9	37.79066833098349	39.0	38.0	39.0	35.0	39.0
10	37.16876810950631	39.0	37.0	39.0	33.0	39.0
11	36.94587269780299	39.0	37.0	39.0	33.0	39.0
12	36.63877536718585	39.0	35.0	39.0	32.0	39.0
13	36.54181214405453	39.0	35.0	39.0	32.0	39.0
14	37.66783418630727	40.0	36.0	41.0	32.0	41.0
15	37.774996322590674	40.0	36.0	41.0	33.0	41.0
16	37.80956397026988	40.0	36.0	41.0	33.0	41.0
17	37.72112331671792	40.0	36.0	41.0	33.0	41.0
18	37.49376956659302	39.0	36.0	41.0	32.0	41.0
19	37.31227658003708	39.0	36.0	41.0	32.0	41.0
20	37.07457473883455	39.0	35.0	41.0	32.0	41.0
21	36.96518325987766	38.0	35.0	41.0	32.0	41.0
22	36.88653596367552	38.0	35.0	41.0	32.0	41.0
23	36.77066357116689	38.0	35.0	40.0	31.0	41.0
24	36.63340634956759	38.0	35.0	40.0	31.0	41.0
25	36.49231907146108	38.0	35.0	40.0	31.0	41.0
26	36.34104675721883	38.0	35.0	40.0	31.0	41.0
27	36.28813577550318	38.0	35.0	40.0	31.0	41.0
28	36.27498313942516	38.0	35.0	40.0	31.0	41.0
29	36.15100032472218	38.0	35.0	40.0	30.0	41.0
30	36.09543675133497	38.0	35.0	40.0	30.0	41.0
31	35.87555265217536	38.0	35.0	40.0	30.0	41.0
32	35.485849953373226	38.0	34.0	40.0	27.0	41.0
33	35.073342112858995	38.0	34.0	40.0	24.0	41.0
34	34.56302975509841	38.0	34.0	40.0	22.0	41.0
35	34.262561128256934	38.0	33.0	40.0	20.0	41.0
36	34.03354872775514	38.0	33.0	40.0	18.0	41.0
37	33.767970042297144	37.0	33.0	40.0	18.0	41.0
38	33.808656760327274	37.0	33.0	40.0	18.0	41.0
39	33.689758831333194	37.0	33.0	40.0	18.0	41.0
40	33.55694294881046	37.0	33.0	40.0	17.0	41.0
41	33.463266496997015	37.0	33.0	40.0	16.0	41.0
42	33.4113393957392	37.0	33.0	40.0	15.0	41.0
43	33.326101904481725	36.0	33.0	40.0	15.0	41.0
44	33.16221642317129	36.0	33.0	40.0	15.0	41.0
45	33.099500635567345	36.0	33.0	40.0	15.0	41.0
46	32.91417655172797	35.0	32.0	40.0	15.0	41.0
47	32.93179134240705	35.0	32.0	40.0	15.0	41.0
48	32.746977238917815	35.0	32.0	40.0	15.0	41.0
49	32.7141435244291	35.0	32.0	40.0	15.0	41.0
50	32.58105044850517	35.0	31.0	40.0	14.0	41.0
51	31.931680326276407	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	6.0
13	12.0
14	32.0
15	120.0
16	274.0
17	780.0
18	1821.0
19	3506.0
20	5962.0
21	9685.0
22	14568.0
23	22077.0
24	33960.0
25	54619.0
26	77316.0
27	83803.0
28	76577.0
29	71647.0
30	75573.0
31	85304.0
32	101951.0
33	128632.0
34	195806.0
35	274688.0
36	244380.0
37	287796.0
38	394617.0
39	636567.0
40	378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.44813881456976	22.65919019283502	26.372540992706238	21.520129999888983
2	38.31173607025101	23.67960189615551	25.345052011057206	12.663610022536275
3	26.07019549940606	23.824720794431432	36.89395600430743	13.21112770185508
4	22.93315718773938	26.147698635611754	36.58949426601686	14.329649910632014
5	21.07953473139647	29.829444530790322	35.52085993094797	13.570160806865236
6	19.309729453689624	37.69427822862662	32.832222709459685	10.163769608224076
7	80.64218668472529	3.3981343739245315	14.346753333259324	1.6129256080908556
8	80.97225151814558	2.8537737158209087	14.15004662677487	2.023928139258634
9	75.6744576862018	4.8748570667317965	16.323326154290218	3.1273590927761803
10	37.28934689210342	25.14841468965441	24.12328480078155	13.438953617460617
11	29.425172352542823	25.224911742176136	29.42801714089057	15.921898764390466
12	24.735122450792097	22.17977397115801	34.7631054542225	18.32199812382739
13	21.088416021847976	26.236650310290084	36.04586215127058	16.62907151659136
14	18.92290762347769	28.22439412946701	36.0315688244502	16.821129422605104
15	18.26624721071972	23.949093553293295	40.756935732761974	17.02772350322502
16	20.771499661400803	22.497522952585012	38.87875095751412	17.85222642850006
17	20.670613752678264	23.391515037134898	34.29860702510075	21.639264185086095
18	22.577350489026056	23.450145431131144	35.46046021736958	18.512043862473217
19	24.572518511939784	25.77607213828169	31.24736336689721	18.40404598288131
20	25.702974954760926	24.607696748337535	31.040942748981426	18.648385547920114
21	23.213334147451626	25.977635800481806	32.616782030929095	18.192248021137473
22	22.869947378354073	23.616218624066075	32.423995581558	21.08983841602185
23	21.3313331927129	27.17858748626175	31.501833153857255	19.98824616716809
24	20.89583772772184	25.297002842012944	35.45726850361358	18.349890926651643
25	21.245989542280494	26.107073670304292	33.689197852948034	18.957738934467177
26	19.899051644703974	27.972422205446453	32.32526061036668	19.803265539482886
27	20.457879092332114	26.43314886152958	33.02750702177026	20.081465024368043
28	19.741859742220544	27.034439979128965	34.61382345104744	18.60987682760305
29	20.415068496952607	25.763201205635177	33.83792477546987	19.983805521942337
30	21.135563184830755	26.829441755387055	33.502239750435734	18.53275530934645
31	23.50343317384016	26.873986977807874	30.696931514149007	18.92564833420296
32	23.983161628384604	26.97972984224608	30.074755486972254	18.96235304239706
33	23.1334025333881	27.450715776502328	29.15359914295547	20.2622825471541
34	21.077800104355163	29.06745756408406	30.04394851071861	19.810793820842168
35	22.27254182532722	28.834913462926163	29.09243619347891	19.800108518267702
36	24.526169277396008	28.969069518301012	26.827152047692532	19.67760915661046
37	22.834248753843934	29.725124060525992	27.669278783707274	19.7713484019228
38	22.38074786016408	30.074998334758043	27.600761015575564	19.943492789502315
39	22.445761681672348	29.193356794742275	27.40169521631493	20.959186307270446
40	23.757868268259376	27.711117987943652	27.543344860508235	20.987668883288745
41	20.5184175760738	27.980748415244733	28.192650454611055	23.308183554070403
42	22.332559920956516	28.61197919557712	27.29602173695838	21.75943914650799
43	22.273409138847875	28.207290706839704	28.325245345648725	21.194054808663697
44	22.410687522897078	29.09455243846931	27.955110627574186	20.539649411059425
45	20.894519411170442	30.07409632869656	26.846822718340974	22.18456154179202
46	22.43781708982315	29.266627440967174	26.93494177203948	21.3606136971702
47	22.047491312987777	28.72091377377133	28.44878548353076	20.782809429710134
48	22.443506666518644	28.0684164659125	28.487502359092776	21.00057450847608
49	21.92249408839104	27.56488892836129	28.54925508176338	21.963361901484284
50	20.6466758995082	29.128204203070705	28.83834802446795	21.38677187295314
51	20.85555968782264	29.628089023835162	27.641524751046326	21.87482653729587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1836.0
1	2481.0
2	3126.0
3	40007.0
4	76888.0
5	52040.0
6	27192.0
7	28621.0
8	30050.0
9	32636.5
10	35223.0
11	37554.5
12	39886.0
13	40392.5
14	40899.0
15	39596.0
16	38293.0
17	36732.0
18	35171.0
19	33100.5
20	31030.0
21	29320.0
22	27610.0
23	27233.5
24	26857.0
25	27238.5
26	28427.0
27	29234.0
28	32445.0
29	35656.0
30	38377.5
31	41099.0
32	46684.5
33	52270.0
34	60421.5
35	68573.0
36	75221.5
37	81870.0
38	90056.0
39	98242.0
40	112844.5
41	127447.0
42	141617.0
43	155787.0
44	166380.0
45	176973.0
46	188986.5
47	201000.0
48	202371.5
49	203743.0
50	200105.5
51	196468.0
52	184787.5
53	173107.0
54	161887.0
55	150667.0
56	141465.5
57	132264.0
58	125459.5
59	118655.0
60	110078.0
61	101501.0
62	91545.5
63	81590.0
64	73376.0
65	65162.0
66	56342.0
67	47522.0
68	40341.5
69	33161.0
70	29267.5
71	25374.0
72	21520.0
73	17666.0
74	14688.0
75	9657.0
76	7604.0
77	5716.5
78	3829.0
79	2622.5
80	1416.0
81	1016.0
82	616.0
83	458.5
84	301.0
85	232.0
86	163.0
87	113.0
88	63.0
89	46.5
90	30.0
91	19.5
92	9.0
93	9.5
94	10.0
95	5.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2882464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.73200643312996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10599350728535	22.92515894991485
2	6.960781440950169	4.139159971638841
3	3.1322821936800507	2.793871029986211
4	1.7966471097786016	2.1367169370400694
5	1.2914240018793615	1.9198313365887807
6	0.9335774779221169	1.665427894764337
7	0.7489512900758455	1.5587477202245217
8	0.6265235864461068	1.4902242642178642
9	0.5324156888597983	1.4246808017650956
>10	6.08590898569134	40.650002620665326
>50	0.7218226764169994	13.874282880389815
>100	0.06059305727162959	2.698098656916411
>500	0.002131604129503903	0.43409633431443306
>1k	8.289571614736804E-4	0.39927426645513875
>5k	0.0	0.0
>10k+	1.184224516390972E-4	1.8904263351184176
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53691	1.8626772094985402	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.469254082618204E-5	0.0	0.0	0.02699079676276963	0.0
2	3.469254082618204E-5	0.0	0.0	0.10553470919324578	0.0
3	3.469254082618204E-5	0.0	0.0	0.20156366220011768	0.0
4	3.469254082618204E-5	0.0	0.0	0.32198147140779554	0.0
5	3.469254082618204E-5	0.0	0.0	0.5745084760815746	0.0
6	3.469254082618204E-5	0.0	0.0	1.21888772938708	0.0
7	3.469254082618204E-5	0.0	0.0	1.6161173218468643	0.0
8	3.469254082618204E-5	0.0	0.0	2.3567683759450246	0.0
9	3.469254082618204E-5	0.0	0.0	2.8492983780543315	0.0
10	1.0407762247854614E-4	0.0	0.0	3.461031950442399	0.0
11	1.0407762247854614E-4	0.0	0.0	3.8101776813170956	0.0
12	1.0407762247854614E-4	0.0	0.0	4.122965629405953	0.0
13	1.0407762247854614E-4	0.0	0.0	4.3576606680950745	0.0
14	1.0407762247854614E-4	0.0	0.0	4.4999000854824205	0.0
15	1.0407762247854614E-4	0.0	0.0	4.600022758306782	0.0
16	1.0407762247854614E-4	0.0	0.0	4.7271015353530865	0.0
17	1.0407762247854614E-4	0.0	0.0	4.856504712634746	0.0
18	1.0407762247854614E-4	0.0	0.0	5.0607397004784795	0.0
19	1.0407762247854614E-4	0.0	0.0	5.165060170742809	0.0
20	1.3877016330472817E-4	0.0	0.0	5.279614940550862	0.0
21	1.3877016330472817E-4	0.0	0.0	5.399789901972756	0.0
22	1.3877016330472817E-4	0.0	0.0	5.527007449182366	0.0
23	1.3877016330472817E-4	0.0	0.0	5.66917748148806	0.0
24	1.3877016330472817E-4	0.0	0.0	5.7931686224008345	0.0
25	1.3877016330472817E-4	0.0	0.0	5.902450126003308	0.0
26	1.3877016330472817E-4	0.0	0.0	6.01301525361635	0.0
27	1.3877016330472817E-4	0.0	0.0	6.136208466090123	0.0
28	1.3877016330472817E-4	1.3877016330472817E-4	0.0	6.254232489980795	0.0
29	1.3877016330472817E-4	1.3877016330472817E-4	0.0	6.380443953506444	0.0
30	1.3877016330472817E-4	1.3877016330472817E-4	0.0	6.564175649721904	0.0
31	1.7346270413091022E-4	1.3877016330472817E-4	0.0	6.704021281792245	0.0
32	1.7346270413091022E-4	1.3877016330472817E-4	0.0	6.857431697325621	0.0
33	1.7346270413091022E-4	1.3877016330472817E-4	0.0	7.003591371826326	0.0
34	1.7346270413091022E-4	1.3877016330472817E-4	0.0	7.149612276163727	0.0
35	1.7346270413091022E-4	1.3877016330472817E-4	0.0	7.304757318738413	0.0
36	1.7346270413091022E-4	1.3877016330472817E-4	0.0	7.452131232168034	0.0
37	1.7346270413091022E-4	1.3877016330472817E-4	0.0	7.606235498517934	0.0
38	1.7346270413091022E-4	1.3877016330472817E-4	0.0	7.77546571266805	0.0
39	1.7346270413091022E-4	1.3877016330472817E-4	0.0	8.026500938086304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34345	0.0	43.5915	1
TATTACG	80	0.0	42.1875	1
GTTTTTT	39220	0.0	38.723866	2
TACGGGA	765	0.0	37.941174	4
CACGACC	1485	0.0	37.424244	27
GCGATAT	325	0.0	37.384617	9
ACACGAC	1490	0.0	36.996647	26
GCGATAC	195	0.0	36.923077	9
GCGTTAG	220	0.0	36.818184	1
TAGGGAC	2375	0.0	36.66316	5
GGGCGAT	7135	0.0	36.485634	7
TATAGCG	105	0.0	36.42857	1
GGCGATA	1350	0.0	36.166668	8
GACACGA	1515	0.0	36.08911	25
CGGTCTA	75	1.8189894E-12	36.000004	31
CGTATGG	425	0.0	36.0	2
AATCGTA	25	0.0021076347	36.0	8
AGGGCGA	3540	0.0	35.911015	6
GCGAGAC	1530	0.0	35.88235	21
ACGGGTA	365	0.0	35.753426	5
>>END_MODULE