FastQCFastQC Report
Thu 26 May 2016
SRR1547677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547677_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2338548
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210550.9003450004019589No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG1050.042.8571431
CGTTTTT132450.041.7044141
CGTTAGG2900.038.7931022
GGGCGAT63650.038.2482347
AGGGCGA33150.037.8733026
GCGATAC1550.037.7419369
CACGACC18850.037.1220227
ATACTAT15950.036.95924845
ACGGGAT4950.036.818185
GGACTAA18950.036.569928
TATACTA16250.036.27692444
GCCCTAC17250.036.2608726
ACTAATC17700.036.22881710
CGACCAA19350.036.0465129
TAATCGT2750.036.021
CTAATCC17900.035.94972211
GACCGAT17150.035.947529
GACTAAT17950.035.8495839
CGTAAGG4150.035.783132
GGCGATA11400.035.7236868
Produced by FastQC (version 0.11.5)