##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547677_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2338548
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.470363233938325	31.0	31.0	33.0	30.0	34.0
2	31.88777053111589	31.0	31.0	34.0	30.0	34.0
3	32.00361378085889	33.0	31.0	34.0	30.0	34.0
4	35.71858392472594	37.0	35.0	37.0	33.0	37.0
5	35.622517476656455	37.0	35.0	37.0	33.0	37.0
6	35.667733140393096	37.0	35.0	37.0	33.0	37.0
7	36.02876998889909	37.0	35.0	37.0	35.0	37.0
8	36.036205799496095	37.0	35.0	37.0	35.0	37.0
9	37.78249281177893	39.0	38.0	39.0	35.0	39.0
10	37.204046699062836	39.0	37.0	39.0	33.0	39.0
11	36.96117719200119	39.0	37.0	39.0	33.0	39.0
12	36.56661184632515	39.0	35.0	39.0	32.0	39.0
13	36.45659229573222	39.0	35.0	39.0	32.0	39.0
14	37.537589136506924	40.0	36.0	41.0	32.0	41.0
15	37.64205310303659	40.0	36.0	41.0	32.0	41.0
16	37.67298126871888	40.0	36.0	41.0	33.0	41.0
17	37.56329312034647	40.0	36.0	41.0	32.0	41.0
18	37.432080504655026	39.0	36.0	41.0	32.0	41.0
19	37.3508985062526	39.0	36.0	41.0	32.0	41.0
20	37.246450789122136	39.0	35.0	41.0	32.0	41.0
21	37.12824966603208	39.0	35.0	41.0	32.0	41.0
22	37.05350798871778	39.0	35.0	41.0	32.0	41.0
23	36.91629079240623	39.0	35.0	41.0	31.0	41.0
24	36.795514994774535	38.0	35.0	41.0	31.0	41.0
25	36.65958321146284	38.0	35.0	40.0	31.0	41.0
26	36.495957320525385	38.0	35.0	40.0	31.0	41.0
27	36.47146691023661	38.0	35.0	40.0	31.0	41.0
28	36.46227958545217	38.0	35.0	40.0	31.0	41.0
29	36.43150792714111	38.0	35.0	41.0	30.0	41.0
30	36.41700320027641	38.0	35.0	41.0	30.0	41.0
31	36.28051508885	38.0	35.0	41.0	30.0	41.0
32	36.04590840128148	38.0	35.0	41.0	30.0	41.0
33	35.85759283110716	38.0	35.0	41.0	29.0	41.0
34	35.557338570771265	38.0	35.0	40.0	27.0	41.0
35	35.384923037714	38.0	34.0	40.0	26.0	41.0
36	35.220979000644846	38.0	34.0	40.0	26.0	41.0
37	34.990004053797485	38.0	34.0	40.0	25.0	41.0
38	35.02674822154602	38.0	34.0	40.0	25.0	41.0
39	34.89696512536839	37.0	34.0	40.0	24.0	41.0
40	34.73364626255266	37.0	34.0	40.0	24.0	41.0
41	34.64353778498453	37.0	34.0	40.0	24.0	41.0
42	34.586450652285095	37.0	34.0	40.0	23.0	41.0
43	34.49484936806942	37.0	33.0	40.0	23.0	41.0
44	34.31611110826034	36.0	33.0	40.0	23.0	41.0
45	34.222545784820326	36.0	33.0	40.0	23.0	41.0
46	34.03677067992618	36.0	33.0	40.0	23.0	41.0
47	34.03230979223005	36.0	33.0	40.0	23.0	41.0
48	33.857809204685985	35.0	33.0	40.0	23.0	41.0
49	33.804704030022044	35.0	33.0	40.0	23.0	41.0
50	33.67071233945166	35.0	33.0	40.0	23.0	41.0
51	32.98411706751369	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	3.0
12	2.0
13	10.0
14	26.0
15	75.0
16	219.0
17	526.0
18	1058.0
19	2113.0
20	3662.0
21	5720.0
22	8881.0
23	12777.0
24	18782.0
25	27825.0
26	38981.0
27	45963.0
28	49146.0
29	52542.0
30	59443.0
31	69913.0
32	85646.0
33	109984.0
34	173911.0
35	235159.0
36	200653.0
37	254917.0
38	353119.0
39	527196.0
40	290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.548509588000755	23.44048529258326	26.838961612077238	16.172043507338742
2	34.38291623691282	24.348356330509358	27.30330957500124	13.96541785757658
3	28.94116349119197	25.696329517290216	31.516436694906414	13.846070296611401
4	25.324474845074807	28.348958413511287	31.126921491455384	15.199645249958522
5	23.26571872803124	31.366429083345732	30.3196684438378	15.048183744785227
6	22.107863511888574	39.9945179658489	27.155440042282645	10.742178479979884
7	86.26348486325703	3.6626145796451475	8.33979033143643	1.7341102256613932
8	86.3350249813132	3.230893699851361	8.313449200101944	2.120632118733505
9	80.82053479338461	5.039451830794151	10.64690568677658	3.493107689044655
10	42.47537360789686	26.11197204419152	17.574067327247505	13.838587020664104
11	34.23872419980261	25.542002986468525	23.69773893886292	16.52153387486594
12	28.34575129524816	23.148252676447097	29.252125677984804	19.253870350319943
13	24.022641399706142	26.95625661735402	30.510940977050716	18.510161005889124
14	21.973292829567747	27.346627052341884	31.767575435697708	18.912504682392665
15	21.410764286215205	22.30794492993088	36.561618576997354	19.71967220685656
16	24.665818276982126	21.580442223123068	33.52204017193575	20.231699327959056
17	24.59936678657013	22.0394877505187	29.531016682146355	23.830128780764817
18	26.851405230938173	21.702825856044004	30.39373149492762	21.0520374180902
19	28.180606085485522	24.102135171054858	26.575849629770264	21.14140911368935
20	30.766612444987228	23.28556009968579	25.927797932734332	20.02002952259265
21	27.882814464359935	24.72072414164687	27.434074476983156	19.96238691701004
22	27.379852797547883	22.090031934345586	27.494197254022584	23.035918014083954
23	25.308781346373905	25.73481493644774	26.939365794501548	22.017037922676806
24	24.632549770199287	23.576039491171443	30.979949951850465	20.811460786778806
25	24.94941305459627	24.41130137162034	28.800306857075412	21.838978716707974
26	23.866091266888684	25.523529985272912	27.744523524853882	22.865855222984518
27	23.364840063150297	24.648585361514925	28.78127795538086	23.20529661995392
28	22.062579002013216	26.068996659465615	29.871911972728377	21.996512365792793
29	23.87939011728645	24.700540677377585	29.05345539197827	22.36661381335769
30	24.515938950151973	24.660173748839025	30.026922688779536	20.79696461222947
31	27.014455123435567	24.99448375658742	26.760109264381143	21.230951855595865
32	27.290181770910838	25.052425693207926	26.432769393657946	21.224623142223294
33	26.15088507911747	25.936307486525827	25.48795235334062	22.424855081016084
34	23.842700684356274	27.09305090167061	27.968337618043332	21.095910795929782
35	24.115348498298943	27.429370703530566	27.236687038281875	21.218593759888616
36	26.319622261334814	28.3179990318779	24.61001441920371	20.75236428758358
37	24.67099242777997	27.62739956588447	25.82705165769529	21.87455634864027
38	25.10827231256318	27.873962817953707	25.106861180527407	21.91090368895571
39	24.942015301802655	26.941888727535208	24.875307241929608	23.240788728732532
40	26.22828353320094	25.587501304228095	25.34679638818617	22.837418774384787
41	22.6836053824852	25.683629328968234	26.200787839291735	25.431977449254838
42	24.797780503115607	25.96944770857814	25.01719015389036	24.215581634415887
43	24.273780140497436	25.532381631679147	26.48694831151638	23.70688991630704
44	24.196681017451855	26.118771134909352	26.7891871366335	22.895360711005292
45	23.069998990826786	26.716834548617346	25.792799634645085	24.420366825910776
46	24.22096959309794	26.341131334486185	26.04714549369951	23.390753578716367
47	23.701886811816564	26.634432990043393	27.52011076958865	22.14356942855139
48	24.375680978111202	25.573518268600857	27.52960383964751	22.52119691364043
49	24.21699276645166	25.204058244688582	27.485388369193192	23.09356061966656
50	23.050285903902765	27.064571691494038	27.64074117785908	22.244401226744117
51	23.253275109170303	26.935731060470005	26.382610064022632	23.42838376633706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1182.0
1	1803.0
2	2424.0
3	18589.5
4	34755.0
5	23907.0
6	13059.0
7	13161.5
8	13264.0
9	13667.5
10	14071.0
11	14165.0
12	14259.0
13	14125.5
14	13992.0
15	13577.0
16	13162.0
17	12692.5
18	12223.0
19	12071.0
20	11919.0
21	11738.0
22	11557.0
23	12201.5
24	12846.0
25	13438.5
26	16288.0
27	18545.0
28	20292.0
29	22039.0
30	25956.5
31	29874.0
32	33017.0
33	36160.0
34	43668.0
35	51176.0
36	56129.0
37	61082.0
38	66475.5
39	71869.0
40	82416.5
41	92964.0
42	104325.5
43	115687.0
44	120113.5
45	124540.0
46	134276.0
47	144012.0
48	148901.5
49	153791.0
50	157006.0
51	160221.0
52	157836.0
53	155451.0
54	151494.5
55	147538.0
56	141696.0
57	135854.0
58	132132.0
59	128410.0
60	123245.5
61	118081.0
62	111140.5
63	104200.0
64	94526.5
65	84853.0
66	73443.0
67	62033.0
68	53405.0
69	44777.0
70	38344.5
71	31912.0
72	27658.5
73	23405.0
74	19893.5
75	13836.5
76	11291.0
77	8274.5
78	5258.0
79	3861.5
80	2465.0
81	1767.5
82	1070.0
83	801.5
84	533.0
85	357.0
86	181.0
87	123.5
88	66.0
89	57.0
90	48.0
91	35.0
92	22.0
93	16.0
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2338548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.576725139009202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.58217611926645	18.575623678913967
2	5.985385322996977	2.9420233986871303
3	2.4737368765156744	1.8238905384107065
4	1.5605268340720104	1.5341055629214406
5	1.170021102671048	1.4377643523593406
6	0.9482941564267782	1.3983578900057712
7	0.8041961853316933	1.383515602331571
8	0.710936911623965	1.3978000854526609
9	0.6274229943300955	1.3878002229790398
>10	9.364445213073957	53.342538514455605
>50	0.7168825049481151	11.071741793030625
>100	0.053159599749943706	2.2390915510947287
>500	0.001936123057836745	0.3284563665284715
>1k	7.040447483042709E-4	0.22649776572409808
>5k	0.0	0.0
>10k+	1.7601118707606772E-4	0.910792677104829
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21055	0.9003450004019589	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2828473052509505E-4	0.0	0.0	0.02621284660396109	0.0
2	1.2828473052509505E-4	0.0	0.0	0.0909538739422924	0.0
3	1.2828473052509505E-4	0.0	0.0	0.16215189938372015	0.0
4	1.2828473052509505E-4	0.0	0.0	0.2516518797133948	0.0
5	1.2828473052509505E-4	0.0	0.0	0.4068764036487598	0.0
6	1.2828473052509505E-4	0.0	0.0	0.838084144520446	0.0
7	1.2828473052509505E-4	0.0	0.0	1.0587338810236095	0.0
8	1.2828473052509505E-4	0.0	0.0	1.5375780184969476	0.0
9	1.2828473052509505E-4	0.0	0.0	1.8440930012982415	0.0
10	1.2828473052509505E-4	0.0	0.0	2.259692766622708	0.0
11	1.2828473052509505E-4	0.0	0.0	2.5381561550158476	0.0
12	1.2828473052509505E-4	0.0	0.0	2.784120745009296	0.0
13	1.2828473052509505E-4	0.0	0.0	2.949437001079302	0.0
14	1.2828473052509505E-4	0.0	0.0	3.0602322466761427	0.0
15	1.2828473052509505E-4	0.0	0.0	3.1434462752100876	0.0
16	1.2828473052509505E-4	0.0	0.0	3.251975157234318	0.0
17	1.2828473052509505E-4	0.0	0.0	3.355885788959645	0.0
18	1.2828473052509505E-4	0.0	0.0	3.5150443779644465	0.0
19	1.2828473052509505E-4	0.0	0.0	3.6105737406288005	0.0
20	2.565694610501901E-4	0.0	0.0	3.7012710451100426	0.0
21	2.565694610501901E-4	0.0	0.0	3.8032146443006516	0.0
22	2.993310378918885E-4	0.0	0.0	3.9092633548680635	0.0
23	2.993310378918885E-4	0.0	0.0	4.0286109158332435	0.0
24	2.993310378918885E-4	0.0	0.0	4.133676110133297	0.0
25	2.993310378918885E-4	0.0	0.0	4.225613500342948	0.0
26	2.993310378918885E-4	0.0	0.0	4.321570478775719	0.0
27	2.993310378918885E-4	0.0	0.0	4.432365724372559	0.0
28	2.993310378918885E-4	0.0	0.0	4.542262976855724	0.0
29	2.993310378918885E-4	0.0	0.0	4.648268925846295	0.0
30	2.993310378918885E-4	0.0	0.0	4.798917961059598	0.0
31	2.993310378918885E-4	0.0	0.0	4.91681162841216	0.0
32	2.993310378918885E-4	0.0	0.0	5.105860559629308	0.0
33	2.993310378918885E-4	0.0	0.0	5.236967554225956	0.0
34	2.993310378918885E-4	0.0	0.0	5.357811770380595	0.0
35	2.993310378918885E-4	0.0	0.0	5.48079406537732	0.0
36	2.993310378918885E-4	0.0	0.0	5.592316257780469	0.0
37	2.993310378918885E-4	0.0	0.0	5.715555122238244	0.0
38	2.993310378918885E-4	0.0	0.0	5.859405066733717	0.0
39	2.993310378918885E-4	0.0	0.0	6.077189777588487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	105	0.0	42.857143	1
CGTTTTT	13245	0.0	41.704414	1
CGTTAGG	290	0.0	38.793102	2
GGGCGAT	6365	0.0	38.248234	7
AGGGCGA	3315	0.0	37.873302	6
GCGATAC	155	0.0	37.741936	9
CACGACC	1885	0.0	37.12202	27
ATACTAT	1595	0.0	36.959248	45
ACGGGAT	495	0.0	36.81818	5
GGACTAA	1895	0.0	36.56992	8
TATACTA	1625	0.0	36.276924	44
GCCCTAC	1725	0.0	36.26087	26
ACTAATC	1770	0.0	36.228817	10
CGACCAA	1935	0.0	36.04651	29
TAATCGT	275	0.0	36.0	21
CTAATCC	1790	0.0	35.949722	11
GACCGAT	1715	0.0	35.94752	9
GACTAAT	1795	0.0	35.849583	9
CGTAAGG	415	0.0	35.78313	2
GGCGATA	1140	0.0	35.723686	8
>>END_MODULE