FastQCFastQC Report
Thu 26 May 2016
SRR1547676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547676_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1441810
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159711.1077048987037126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116950.043.672511
ACGGGTA2250.041.05
TAGTACG403.4590448E-739.3751
GTTTTTT135650.038.3984532
AGGGCGA18150.038.181826
TACGGGA2600.038.076924
GCGTAAG1300.038.076921
CGTTAGG1550.037.741942
TCAAGCG17350.037.7377517
ACACGAC17000.037.7205926
CACGACC17050.037.60997427
TAGGGCG7200.037.55
ACGACCA17250.037.4347828
AACACGT16450.037.34042441
GGGCGAT35050.037.1041377
ATAGGGC8250.037.0909084
AGACACG17300.037.06647524
GCGAGAC17450.037.0057321
CGACCAA17500.036.89999829
CGTTCGG2200.036.8181845
Produced by FastQC (version 0.11.5)