FastQCFastQC Report
Thu 26 May 2016
SRR1547675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547675_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4084813
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201710.49380473475774783No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC65670.16076623336245746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG850.039.7058831
CGTTTTT130450.039.6876221
CGTAAGG7550.039.0397342
TACGGGA9600.038.9062464
GCGTAAG4800.038.9062461
AGGGCGA58950.038.8167956
TGTTACG2450.037.653061
GGCGATA23250.037.645168
GCGATAT7000.037.6071439
GACCGAT29400.037.193889
GGGCGAT114550.036.927117
TAACGGG11300.036.8362853
CGCAATA552.752131E-936.8181818
CGTTCGA1050.036.42857414
TAGGGAC23200.036.0775835
ATACGAG2250.036.01
CGTTAGG4600.035.706522
ATAGGGC24300.035.5555534
AACACGT7200.035.312541
TTAGGGA31050.035.2898564
Produced by FastQC (version 0.11.5)