##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547675_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4084813
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389412440667417	31.0	31.0	33.0	30.0	34.0
2	31.799086029152374	31.0	31.0	34.0	30.0	34.0
3	31.949482387565844	31.0	31.0	34.0	30.0	34.0
4	35.65064055563865	37.0	35.0	37.0	33.0	37.0
5	35.5266434473255	37.0	35.0	37.0	33.0	37.0
6	35.57608928487057	37.0	35.0	37.0	33.0	37.0
7	35.96507379897195	37.0	35.0	37.0	35.0	37.0
8	35.97357406569162	37.0	35.0	37.0	35.0	37.0
9	37.71122937573887	39.0	37.0	39.0	35.0	39.0
10	37.101600734231894	39.0	37.0	39.0	33.0	39.0
11	36.82141826321058	39.0	37.0	39.0	32.0	39.0
12	36.25132411202178	38.0	35.0	39.0	32.0	39.0
13	36.01525871563766	38.0	35.0	39.0	31.0	39.0
14	37.04888204184623	39.0	35.0	41.0	31.0	41.0
15	37.22441688273123	39.0	35.0	41.0	32.0	41.0
16	37.31295214738104	39.0	35.0	41.0	32.0	41.0
17	37.25447603109371	39.0	35.0	41.0	32.0	41.0
18	37.1488893126809	39.0	35.0	41.0	32.0	41.0
19	37.10178238269414	39.0	35.0	41.0	32.0	41.0
20	37.01963786347135	39.0	35.0	41.0	32.0	41.0
21	36.8748598773065	38.0	35.0	41.0	31.0	41.0
22	36.776084241800056	38.0	35.0	41.0	31.0	41.0
23	36.67192500611411	38.0	35.0	41.0	31.0	41.0
24	36.54879354330296	38.0	35.0	40.0	31.0	41.0
25	36.38897986272566	38.0	35.0	40.0	31.0	41.0
26	36.219776523429594	38.0	35.0	40.0	30.0	41.0
27	36.19523243781294	38.0	35.0	40.0	30.0	41.0
28	36.224631825251244	38.0	35.0	40.0	30.0	41.0
29	36.2069441122519	38.0	35.0	40.0	30.0	41.0
30	36.23772422385064	38.0	35.0	40.0	30.0	41.0
31	36.119187341011695	38.0	35.0	40.0	30.0	41.0
32	35.92599122652616	38.0	35.0	40.0	30.0	41.0
33	35.819294542981524	38.0	35.0	40.0	29.0	41.0
34	35.624687837607254	38.0	35.0	40.0	29.0	41.0
35	35.525047046217296	38.0	34.0	40.0	28.0	41.0
36	35.37390769173521	38.0	34.0	40.0	27.0	41.0
37	35.10620828909426	37.0	34.0	40.0	26.0	41.0
38	35.17228279483051	37.0	34.0	40.0	27.0	41.0
39	35.04098008892941	37.0	34.0	40.0	26.0	41.0
40	34.86559898825234	37.0	34.0	40.0	25.0	41.0
41	34.79387844682241	37.0	34.0	40.0	25.0	41.0
42	34.73960937746722	37.0	34.0	40.0	25.0	41.0
43	34.650151181951294	36.0	34.0	40.0	25.0	41.0
44	34.48487140047782	36.0	33.0	40.0	24.0	41.0
45	34.423709481927325	36.0	33.0	40.0	24.0	41.0
46	34.23829413978069	36.0	33.0	40.0	23.0	41.0
47	34.24430077949713	35.0	33.0	40.0	24.0	41.0
48	34.07799255437152	35.0	33.0	40.0	23.0	41.0
49	34.04425490224399	35.0	33.0	40.0	24.0	41.0
50	33.877881313049095	35.0	33.0	39.0	24.0	41.0
51	33.16750485273133	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	4.0
12	6.0
13	12.0
14	44.0
15	135.0
16	382.0
17	1046.0
18	2264.0
19	4256.0
20	7368.0
21	11196.0
22	16523.0
23	23963.0
24	33866.0
25	46210.0
26	59310.0
27	70829.0
28	79348.0
29	91196.0
30	106895.0
31	128827.0
32	158038.0
33	203217.0
34	333173.0
35	494825.0
36	330169.0
37	409650.0
38	570413.0
39	901100.0
40	541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.753711614215874	24.17258758234465	28.503801765221564	13.569899038217907
2	30.857716130456893	26.0780603665333	28.9386074711376	14.125616031872207
3	28.524586070402734	26.00273256082959	31.395096911413077	14.077584457354597
4	25.503174808736656	28.605226236794685	30.195213342691574	15.696385611777085
5	23.53613249859908	32.378691509256356	29.262269778322782	14.822906213821781
6	22.14723170926062	41.26039551871774	25.86732856559162	10.725044206430013
7	87.48410269943814	4.483852749195618	6.192670264220173	1.8393742871460699
8	87.90625176721676	3.772926692115404	5.891163194006678	2.429658346661157
9	82.51467080622785	5.465660239526264	8.411156153292696	3.608512800953189
10	47.04122318451297	23.521394981851067	14.938994759368422	14.498387074267537
11	38.301826791091784	23.943397164080707	21.55143944165865	16.20333660316886
12	33.92968050189813	22.43258136908593	25.639533560043017	17.998204568972927
13	23.82226554801897	30.933509073732385	27.450338607911796	17.793886770336854
14	20.634947058776007	32.63916854945379	28.559251059962843	18.166633331807354
15	19.56378419281372	23.324005284942054	39.082009384517725	18.0302011377265
16	21.516333795451594	21.061184441001338	36.93407752080695	20.48840424274012
17	22.785204610345687	21.060229684932946	27.38370153052294	28.77086417419843
18	24.8490444972634	23.06940366670396	30.16255578896757	21.918996047065068
19	28.331725344587376	25.493088667706452	24.982881713312214	21.192304274393955
20	30.669849513306975	23.931940091259992	24.010230088868205	21.38798030656483
21	26.464565207758596	26.574925216894872	26.236990530533465	20.72351904481307
22	26.574533522097582	23.722750588582635	25.534339026045988	24.168376863273792
23	24.0990713650784	27.685943028481354	24.339890222636875	23.875095383803373
24	24.873451979319494	24.266741219243084	30.943619695687413	19.916187105750005
25	22.861095477320504	25.125238291202066	28.872655859644002	23.141010371833424
26	21.30366800144829	29.388273098425806	26.24827623688037	23.05978266324554
27	22.097706798328346	27.966665793513677	26.959814317081342	22.97581309107663
28	20.600233107366236	26.951050145991996	31.343686969268852	21.10502977737292
29	21.37304694241817	24.453628599399778	31.21004560061868	22.963278857563367
30	22.60061843712307	26.382235857553333	28.99116801674887	22.025977688574727
31	26.78061394732146	26.502535122170833	24.555542689469505	22.161308241038206
32	27.850577247967045	26.138748579188327	24.372817066534015	21.63785710631062
33	26.764627903407085	27.029242219900883	24.26610471519749	21.940025161494543
34	22.15770954508811	26.752509845615947	27.052205327391977	24.03757528190397
35	22.466291602577645	26.227100236901908	28.16880478983983	23.137803370680615
36	27.15801188450977	25.88825975632177	25.271683183538634	21.682045175629828
37	23.528984068548546	28.156784655748012	26.34409457666728	21.97013669903616
38	23.623235629146304	28.117003152898306	24.977887604646774	23.281873613308615
39	23.66776643141314	28.235368424454194	24.644579813078344	23.452285331054323
40	24.950468968836518	25.369851692109286	25.557008362439117	24.122670976615083
41	21.574231182675927	24.485796534627166	26.949801618825635	26.99017066387127
42	24.31998723074961	25.92588693778638	24.699588451172673	25.054537380291336
43	23.68071684064852	25.731802165729494	26.250577443814443	24.33690354980754
44	23.314384281483633	27.642146653959436	26.4424834135614	22.60098565099553
45	21.83294559628556	29.20016167202758	24.869706397820416	24.097186333866446
46	23.35756863288479	28.55702329580326	25.886717458057447	22.198690613254513
47	23.21655850586061	26.31447265762227	27.048459745892895	23.420509090624222
48	24.508588275644442	24.799029967834514	27.39040930392652	23.301972452594523
49	23.185002593753985	24.295237015745887	28.259751425585456	24.26000896491467
50	22.01848652557657	28.37444455841675	27.235714339922048	22.371354576084634
51	21.9558398389351	29.338895073042508	25.271609740764145	23.43365534725825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2646.0
1	3526.5
2	4407.0
3	18370.5
4	32334.0
5	22633.5
6	12933.0
7	13831.0
8	14729.0
9	16385.5
10	18042.0
11	18437.0
12	18832.0
13	19163.0
14	19494.0
15	19247.0
16	19000.0
17	18375.5
18	17751.0
19	17540.5
20	17330.0
21	17854.5
22	18379.0
23	18760.0
24	19141.0
25	19664.0
26	26225.0
27	32263.0
28	35179.0
29	38095.0
30	43332.0
31	48569.0
32	55777.5
33	62986.0
34	73698.0
35	84410.0
36	92520.5
37	100631.0
38	119335.0
39	138039.0
40	171217.5
41	204396.0
42	229224.5
43	254053.0
44	270367.5
45	286682.0
46	287534.5
47	288387.0
48	289746.0
49	291105.0
50	304295.5
51	317486.0
52	304238.5
53	290991.0
54	268565.0
55	246139.0
56	232631.5
57	219124.0
58	210117.5
59	201111.0
60	188352.5
61	175594.0
62	165162.5
63	154731.0
64	139917.5
65	125104.0
66	111433.5
67	97763.0
68	82486.5
69	67210.0
70	56304.0
71	45398.0
72	39537.5
73	33677.0
74	27501.5
75	15991.0
76	10656.0
77	8691.0
78	6726.0
79	5163.0
80	3600.0
81	2767.0
82	1934.0
83	1386.0
84	838.0
85	598.0
86	358.0
87	251.0
88	144.0
89	96.0
90	48.0
91	33.5
92	19.0
93	13.5
94	8.0
95	6.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4084813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.058723124453376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24937514303596	15.485093448859665
2	7.01203048275953	2.6728069502228204
3	2.326867358285539	1.3304136218667704
4	1.162462066720805	0.8862017068924668
5	0.7516934577722396	0.716315874307205
6	0.5243801480107012	0.59964096317375
7	0.39726176981803385	0.5299911455224565
8	0.30821390506701607	0.4699330783823053
9	0.25175130408155627	0.43182525606394045
>10	3.287942183132824	15.038400982232917
>50	1.317586685873279	18.276006892415463
>100	1.4059881243608119	42.18251018366383
>500	0.003139320763537618	0.39882644200304757
>1k	0.0010464402545125391	0.31546129498103287
>5k	1.308050318140674E-4	0.1637137920136275
>10k+	1.308050318140674E-4	0.5028583673986419
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20171	0.49380473475774783	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6567	0.16076623336245746	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.344277449175764E-5	0.0	0.0	0.028324430028987862	0.0
2	7.344277449175764E-5	0.0	0.0	0.10411737330448174	0.0
3	7.344277449175764E-5	0.0	0.0	0.16830635821027792	0.0
4	7.344277449175764E-5	0.0	0.0	0.3069418355258858	0.0
5	7.344277449175764E-5	0.0	0.0	0.5256788988871706	0.0
6	7.344277449175764E-5	0.0	0.0	1.011821104172945	0.0
7	7.344277449175764E-5	0.0	0.0	1.2547942831165098	0.0
8	7.344277449175764E-5	0.0	0.0	1.9288520674018614	0.0
9	7.344277449175764E-5	0.0	0.0	2.2820138890078936	0.0
10	7.344277449175764E-5	0.0	0.0	2.7777526168272573	0.0
11	7.344277449175764E-5	0.0	0.0	3.1156628222638343	0.0
12	7.344277449175764E-5	0.0	0.0	3.407597850868571	0.0
13	7.344277449175764E-5	0.0	0.0	3.5751453004091007	0.0
14	7.344277449175764E-5	0.0	0.0	3.6626401257536148	0.0
15	7.344277449175764E-5	0.0	0.0	3.737576236660038	0.0
16	9.792369932234351E-5	0.0	0.0	3.858487524398302	0.0
17	9.792369932234351E-5	0.0	0.0	3.983389202883951	0.0
18	9.792369932234351E-5	0.0	0.0	4.18310458765187	0.0
19	9.792369932234351E-5	0.0	0.0	4.276352430331572	0.0
20	1.4688554898351528E-4	0.0	0.0	4.381277674155463	0.0
21	1.7136647381410115E-4	0.0	0.0	4.501821748021268	0.0
22	2.692901731364447E-4	0.0	0.0	4.626943754830393	0.0
23	2.9377109796703056E-4	0.0	0.0	4.771528096879833	0.0
24	3.1825202279761643E-4	0.0	0.0	4.89466714877768	0.0
25	3.1825202279761643E-4	0.0	0.0	5.001918080460476	0.0
26	3.1825202279761643E-4	0.0	0.0	5.109805516188868	0.0
27	3.427329476282023E-4	0.0	0.0	5.216909562322682	0.0
28	3.427329476282023E-4	0.0	0.0	5.3227895622149655	0.0
29	3.427329476282023E-4	0.0	0.0	5.4411303528460175	0.0
30	3.427329476282023E-4	0.0	0.0	5.609510153830787	0.0
31	3.672138724587882E-4	0.0	0.0	5.732796091277618	0.0
32	3.672138724587882E-4	0.0	0.0	5.859852091148358	0.0
33	3.672138724587882E-4	0.0	0.0	5.98316250952002	0.0
34	3.672138724587882E-4	0.0	0.0	6.108039707080838	0.0
35	3.672138724587882E-4	0.0	0.0	6.25683476820114	0.0
36	3.9169479728937406E-4	0.0	0.0	6.3861430131562935	0.0
37	3.9169479728937406E-4	0.0	0.0	6.522477283537826	0.0
38	3.9169479728937406E-4	0.0	0.0	6.676437819797381	0.0
39	3.9169479728937406E-4	0.0	0.0	6.889862522470429	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	85	0.0	39.705883	1
CGTTTTT	13045	0.0	39.687622	1
CGTAAGG	755	0.0	39.039734	2
TACGGGA	960	0.0	38.906246	4
GCGTAAG	480	0.0	38.906246	1
AGGGCGA	5895	0.0	38.816795	6
TGTTACG	245	0.0	37.65306	1
GGCGATA	2325	0.0	37.64516	8
GCGATAT	700	0.0	37.607143	9
GACCGAT	2940	0.0	37.19388	9
GGGCGAT	11455	0.0	36.92711	7
TAACGGG	1130	0.0	36.836285	3
CGCAATA	55	2.752131E-9	36.81818	18
CGTTCGA	105	0.0	36.428574	14
TAGGGAC	2320	0.0	36.077583	5
ATACGAG	225	0.0	36.0	1
CGTTAGG	460	0.0	35.70652	2
ATAGGGC	2430	0.0	35.555553	4
AACACGT	720	0.0	35.3125	41
TTAGGGA	3105	0.0	35.289856	4
>>END_MODULE