FastQCFastQC Report
Thu 26 May 2016
SRR1547674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547674_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1293637
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61450.4750173348474108No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.0336147E-445.00000444
CGTTTTT52550.042.6022831
TTAACGG1300.039.807692
CGTAAGG2100.039.642862
TAATGCG800.039.3750041
AATGCGG1750.038.5714262
CACGACC7400.037.702727
AGGGCGA17600.037.5852286
ATTCGGC1200.037.49999617
AACACGT6350.037.20472341
GCGCGAC1150.037.1739169
TATTGCG850.037.0588231
GGGCGAT32950.037.010627
AGTGTCG552.750312E-936.818181
TAAGGGC9850.036.7766464
GACACGA7600.036.7105225
AGGGTAT10850.036.705076
GCGAGAC7800.036.63461721
AGGGTAC8050.036.614916
GGCGATA7650.036.470598
Produced by FastQC (version 0.11.5)