##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547674_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1293637
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.425985805910003	31.0	31.0	33.0	30.0	34.0
2	31.84323423031345	31.0	31.0	34.0	30.0	34.0
3	31.995687352789073	33.0	31.0	34.0	30.0	34.0
4	35.70322663931226	37.0	35.0	37.0	33.0	37.0
5	35.59837033108979	37.0	35.0	37.0	33.0	37.0
6	35.65758864349118	37.0	35.0	37.0	33.0	37.0
7	36.02527370506564	37.0	35.0	37.0	35.0	37.0
8	36.04239520050833	37.0	35.0	37.0	35.0	37.0
9	37.822889264917436	39.0	38.0	39.0	35.0	39.0
10	37.09638638969046	39.0	37.0	39.0	33.0	39.0
11	36.91807516327996	39.0	37.0	39.0	33.0	39.0
12	36.46155065138056	39.0	35.0	39.0	32.0	39.0
13	36.317030202444734	39.0	35.0	39.0	32.0	39.0
14	37.40319656905299	39.0	35.0	41.0	32.0	41.0
15	37.5313383893627	39.0	35.0	41.0	32.0	41.0
16	37.57291187558798	39.0	35.0	41.0	33.0	41.0
17	37.49713946029682	39.0	35.0	41.0	32.0	41.0
18	37.405486237638534	39.0	36.0	41.0	32.0	41.0
19	37.361385767413886	39.0	35.0	41.0	32.0	41.0
20	37.255653633901936	39.0	35.0	41.0	32.0	41.0
21	37.165950726517565	39.0	35.0	41.0	32.0	41.0
22	37.074271221370445	39.0	35.0	41.0	32.0	41.0
23	36.97147422344908	39.0	35.0	41.0	32.0	41.0
24	36.876491627867786	38.0	35.0	41.0	31.0	41.0
25	36.747613897870885	38.0	35.0	41.0	31.0	41.0
26	36.60764186553106	38.0	35.0	40.0	31.0	41.0
27	36.58154258111047	38.0	35.0	41.0	31.0	41.0
28	36.60916624988308	38.0	35.0	41.0	31.0	41.0
29	36.59570961560314	38.0	35.0	41.0	31.0	41.0
30	36.61395275490729	38.0	35.0	41.0	31.0	41.0
31	36.507551963958974	38.0	35.0	41.0	31.0	41.0
32	36.30141376599463	38.0	35.0	41.0	30.0	41.0
33	36.19818156097885	38.0	35.0	41.0	30.0	41.0
34	35.98513647955338	38.0	35.0	41.0	30.0	41.0
35	35.870691700994946	38.0	35.0	41.0	29.0	41.0
36	35.727490787601155	38.0	35.0	41.0	29.0	41.0
37	35.474889787475156	38.0	34.0	40.0	27.0	41.0
38	35.5527802621601	38.0	35.0	40.0	28.0	41.0
39	35.46165964640776	38.0	35.0	40.0	27.0	41.0
40	35.330733428311035	38.0	34.0	40.0	27.0	41.0
41	35.28080365666721	38.0	34.0	40.0	26.0	41.0
42	35.25053937078176	38.0	34.0	40.0	27.0	41.0
43	35.17456442572375	38.0	34.0	40.0	26.0	41.0
44	35.035094852729166	38.0	34.0	40.0	26.0	41.0
45	34.97012685938946	37.0	34.0	40.0	26.0	41.0
46	34.79165561900286	37.0	34.0	40.0	25.0	41.0
47	34.81534232555191	37.0	34.0	40.0	26.0	41.0
48	34.66073249296364	37.0	34.0	40.0	25.0	41.0
49	34.63320390495943	37.0	34.0	40.0	25.0	41.0
50	34.50143432817707	36.0	34.0	40.0	24.0	41.0
51	33.80016109619623	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	10.0
15	36.0
16	74.0
17	223.0
18	506.0
19	993.0
20	1685.0
21	2765.0
22	4279.0
23	6262.0
24	9560.0
25	13967.0
26	18856.0
27	21666.0
28	22860.0
29	25501.0
30	30085.0
31	36449.0
32	45420.0
33	59655.0
34	95828.0
35	141262.0
36	100586.0
37	128215.0
38	191484.0
39	335208.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.84661771424287	24.385898053317895	28.63933236294262	14.128151869496621
2	31.297342299269427	25.19176554164731	29.567799931510923	13.94309222757234
3	28.14352094134599	25.430085874167176	32.1860769288448	14.240316255642039
4	24.08256721166757	28.203661459899493	32.37600656134603	15.337764767086902
5	21.974247799034817	32.46227496585209	31.231249570010753	14.332227665102343
6	19.873349324424087	41.590183335819866	28.14993696067753	10.38653037907852
7	89.0689582935553	3.4327249452512567	6.210242904307777	1.2880738568856642
8	90.44476928226388	2.3372089697496286	5.715745607152548	1.5022761408339433
9	85.310253185399	4.151087206070946	7.8637979587782345	2.674861649751824
10	41.07953003817918	22.016840891223737	20.889012914751202	16.014616155845882
11	36.52090965239863	26.45239738813902	19.97778356679656	17.048909392665795
12	29.98422277655942	24.04121094248232	26.288054531526235	19.68651174943203
13	23.02562465359293	31.10362489631945	28.162846300778348	17.70790414930927
14	21.18345409106264	32.27265453910177	28.786359697504015	17.757531672331574
15	20.880123249412318	24.882946297918195	36.40866796481548	17.828262487854012
16	21.831858550737184	22.729560147089174	36.01883681434591	19.419744487827728
17	22.420740903360063	23.49306644754286	28.455741448335196	25.630451200761883
18	25.332376856877158	23.865736678836488	30.077525611898853	20.724360852387495
19	27.307196686551173	26.608855498103413	25.807007684535925	20.276940130809493
20	28.487821544992915	25.703732963729394	25.50514557020246	20.303299921075233
21	25.60695156369213	26.80821590600764	27.50825772608545	20.076574804214783
22	24.79389504165388	24.155385166008703	27.442860709766343	23.607859082571075
23	23.379819841269228	28.50676039723663	26.087998410682438	22.0254213508117
24	23.31202648038051	26.011083480141643	31.132458332592527	19.544431706885316
25	21.964507817880904	26.188490279730715	30.23143277441817	21.61556912797021
26	21.48454319101881	29.073302634355695	27.547449555014275	21.89470461961122
27	21.808049707916517	28.04380208667501	28.22770220703335	21.920445998375122
28	20.23975813926163	28.21765302012852	31.532415971404653	20.010172869205196
29	20.962062773405524	26.102608382413305	31.139570064863637	21.795758779317538
30	22.2068478251627	26.845707103306417	30.400491018732456	20.546954052798426
31	25.42668461090708	26.371926591462675	27.35566468800753	20.845724109622715
32	25.68023332665964	25.999101757293587	27.074364756110096	21.246300159936673
33	24.614091897495204	26.99312094505646	26.576311592819312	21.816475564629027
34	21.098886318186633	27.886802866646516	28.35888274686021	22.655428068306644
35	21.774655486817398	27.479733495563284	28.533661297566475	22.211949720052843
36	25.68340268560655	27.24295919179801	25.450648056603203	21.622990065992237
37	22.470213823506903	29.303274411600782	27.207014023253816	21.019497741638496
38	22.565526496227303	29.490730398094673	25.33624192876363	22.60750117691439
39	22.997255025946227	28.7508783375862	25.140823894183605	23.111042742283963
40	24.024281927619572	26.7353979516665	25.236523074092652	24.00379704662127
41	20.957733892892673	26.125721512294408	26.648124628469965	26.268419966342954
42	23.421717220518584	27.25123044563506	25.03128775692099	24.295764576925365
43	22.677690882372723	26.883894013544758	26.769024077078807	23.669391027003712
44	23.072237420543786	28.313042994286654	26.573064932434676	22.041654652734884
45	21.59469773978326	29.976183427035558	24.71512487660758	23.7139939565736
46	22.801295881302096	28.904089787165955	26.20163152414472	22.092982807387234
47	22.734739343417047	27.182741371806774	27.37970543514139	22.70281384963479
48	23.654935658148307	26.132446737376867	27.72995824949348	22.482659354981344
49	22.870635270945407	25.729087835304647	27.953823213157943	23.446453680592004
50	21.924311070261595	28.527399881110387	26.807133685879425	22.741155362748593
51	21.284950878801396	29.22465884942994	25.828961292851087	23.661428978917577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	539.0
1	711.0
2	883.0
3	5632.0
4	10381.0
5	7449.5
6	4518.0
7	4783.5
8	5049.0
9	5412.0
10	5775.0
11	5933.0
12	6091.0
13	6121.5
14	6152.0
15	6111.5
16	6071.0
17	5894.0
18	5717.0
19	5482.0
20	5247.0
21	5578.5
22	5910.0
23	5998.5
24	6087.0
25	6640.5
26	8095.0
27	8996.0
28	10729.5
29	12463.0
30	14484.5
31	16506.0
32	19565.5
33	22625.0
34	25991.0
35	29357.0
36	33977.0
37	38597.0
38	44545.0
39	50493.0
40	61288.5
41	72084.0
42	81703.5
43	91323.0
44	94331.0
45	97339.0
46	97407.5
47	97476.0
48	99214.0
49	100952.0
50	104522.5
51	108093.0
52	104084.5
53	100076.0
54	91691.0
55	83306.0
56	75262.5
57	67219.0
58	62453.0
59	57687.0
60	52721.0
61	47755.0
62	42832.5
63	37910.0
64	32304.5
65	26699.0
66	22265.5
67	17832.0
68	14416.5
69	11001.0
70	9515.0
71	8029.0
72	6827.0
73	5625.0
74	4813.0
75	3256.5
76	2512.0
77	1881.5
78	1251.0
79	886.5
80	522.0
81	347.5
82	173.0
83	127.5
84	82.0
85	52.0
86	22.0
87	14.0
88	6.0
89	5.5
90	5.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1293637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.36938879129128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5449003586134	20.402483603229587
2	6.170863997938564	3.3778555187552524
3	2.9047419120994484	2.3850303219182587
4	1.9544336728951512	2.1396662024103517
5	1.4917537040664777	2.0414193553722146
6	1.1931967699983475	1.9594239781558636
7	1.032454646710269	1.9780356844632048
8	0.8793469487400044	1.9253750818000688
9	0.7444393511259013	1.8337365029251256
>10	8.478027507915671	50.45039113822103
>50	0.5813923331088742	9.962249835522243
>100	0.023880220120087937	0.9680868817505579
>500	0.0	0.0
>1k	2.842883339438619E-4	0.09811586203018424
>5k	2.842883339438619E-4	0.47813003344606037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6145	0.4750173348474108	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03300771391047102	0.0
2	0.0	0.0	0.0	0.09562187847131769	0.0
3	0.0	0.0	0.0	0.15707652146622275	0.0
4	0.0	0.0	0.0	0.24056207421401832	0.0
5	0.0	0.0	0.0	0.3760714945537272	0.0
6	0.0	0.0	0.0	0.7320446152978	0.0
7	0.0	0.0	0.0	0.9037311084948869	0.0
8	0.0	0.0	0.0	1.3346093223987874	0.0
9	0.0	0.0	0.0	1.5885445453399987	0.0
10	0.0	0.0	0.0	1.9656209585842086	0.0
11	0.0	0.0	0.0	2.273899092249217	0.0
12	0.0	0.0	0.0	2.555740134210756	0.0
13	0.0	0.0	0.0	2.7459016710251793	0.0
14	0.0	0.0	0.0	2.850258611959924	0.0
15	0.0	0.0	0.0	2.940933198416557	0.0
16	0.0	0.0	0.0	3.0673983505419216	0.0
17	0.0	0.0	0.0	3.2023666608175247	0.0
18	0.0	0.0	0.0	3.3977073939598204	0.0
19	0.0	0.0	0.0	3.496653234253504	0.0
20	7.730143772944032E-5	0.0	0.0	3.6047206441992614	0.0
21	7.730143772944032E-5	0.0	0.0	3.733118332267862	0.0
22	1.5460287545888065E-4	0.0	0.0	3.873033934558149	0.0
23	1.5460287545888065E-4	0.0	0.0	4.032970609220361	0.0
24	1.5460287545888065E-4	0.0	0.0	4.171494785631518	0.0
25	1.5460287545888065E-4	0.0	0.0	4.293476454368575	0.0
26	1.5460287545888065E-4	0.0	0.0	4.410665433966407	0.0
27	1.5460287545888065E-4	0.0	0.0	4.536202968839017	0.0
28	1.5460287545888065E-4	0.0	0.0	4.674108733748339	0.0
29	1.5460287545888065E-4	0.0	0.0	4.808690536835295	0.0
30	1.5460287545888065E-4	7.730143772944032E-5	0.0	4.98470591054523	0.0
31	1.5460287545888065E-4	7.730143772944032E-5	0.0	5.124312307084599	0.0
32	1.5460287545888065E-4	7.730143772944032E-5	0.0	5.287882149320096	0.0
33	1.5460287545888065E-4	7.730143772944032E-5	0.0	5.432745043625066	0.0
34	1.5460287545888065E-4	7.730143772944032E-5	0.0	5.577376033616849	0.0
35	1.5460287545888065E-4	7.730143772944032E-5	0.0	5.735534775211284	0.0
36	1.5460287545888065E-4	7.730143772944032E-5	0.0	5.880861478142632	0.0
37	1.5460287545888065E-4	7.730143772944032E-5	0.0	6.043812908876292	0.0
38	1.5460287545888065E-4	7.730143772944032E-5	0.0	6.216504320763862	0.0
39	1.5460287545888065E-4	7.730143772944032E-5	0.0	6.429778987459388	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.0336147E-4	45.000004	44
CGTTTTT	5255	0.0	42.602283	1
TTAACGG	130	0.0	39.80769	2
CGTAAGG	210	0.0	39.64286	2
TAATGCG	80	0.0	39.375004	1
AATGCGG	175	0.0	38.571426	2
CACGACC	740	0.0	37.7027	27
AGGGCGA	1760	0.0	37.585228	6
ATTCGGC	120	0.0	37.499996	17
AACACGT	635	0.0	37.204723	41
GCGCGAC	115	0.0	37.173916	9
TATTGCG	85	0.0	37.058823	1
GGGCGAT	3295	0.0	37.01062	7
AGTGTCG	55	2.750312E-9	36.81818	1
TAAGGGC	985	0.0	36.776646	4
GACACGA	760	0.0	36.71052	25
AGGGTAT	1085	0.0	36.70507	6
GCGAGAC	780	0.0	36.634617	21
AGGGTAC	805	0.0	36.61491	6
GGCGATA	765	0.0	36.47059	8
>>END_MODULE