FastQCFastQC Report
Thu 26 May 2016
SRR1547673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547673_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4159451
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516181.240981081397521No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT386650.042.9516331
AGGGCGA56750.037.8237886
GGCGATA23150.037.8077778
GGGCGAT114150.037.6872527
GTTTTTT450600.037.6697732
CGTTCGG11100.037.545
ACACGAC22350.036.74496526
ATAGGGC22650.036.655634
CACGACC22600.036.43805327
CATACGA5950.036.30252518
ACGACCA22900.035.96069728
TATAGCG2450.035.8163261
GACACGA23050.035.7266825
CGTAAGG7550.035.4635772
AACACGT21500.035.37209341
ATACTAT26050.035.32629445
TAAGGGA37200.035.3225824
GACCGAT30300.035.2722749
GCGAGAC23550.035.06369421
TAGGGCG24050.034.896055