##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547673_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4159451
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42058387032327	31.0	31.0	33.0	30.0	34.0
2	31.83820941754092	31.0	31.0	34.0	30.0	34.0
3	31.919599004772504	31.0	31.0	34.0	30.0	34.0
4	35.65867154102789	37.0	35.0	37.0	33.0	37.0
5	35.56096754114906	37.0	35.0	37.0	33.0	37.0
6	35.60920876336805	37.0	35.0	37.0	33.0	37.0
7	36.014169177615024	37.0	35.0	37.0	35.0	37.0
8	36.02353940459931	37.0	35.0	37.0	35.0	37.0
9	37.76249606017717	39.0	38.0	39.0	35.0	39.0
10	37.11444755569906	39.0	37.0	39.0	33.0	39.0
11	36.93983148256825	39.0	37.0	39.0	33.0	39.0
12	36.635886322497846	39.0	35.0	39.0	32.0	39.0
13	36.54092427101558	39.0	35.0	39.0	32.0	39.0
14	37.61747091142557	40.0	36.0	41.0	32.0	41.0
15	37.70331156683899	40.0	36.0	41.0	32.0	41.0
16	37.71655153528675	40.0	36.0	41.0	33.0	41.0
17	37.61837848312193	40.0	36.0	41.0	32.0	41.0
18	37.43722044087068	39.0	36.0	41.0	32.0	41.0
19	37.32312533553106	39.0	36.0	41.0	32.0	41.0
20	37.14078396403756	39.0	35.0	41.0	32.0	41.0
21	37.03875295081009	39.0	35.0	41.0	32.0	41.0
22	36.96380772366353	39.0	35.0	41.0	32.0	41.0
23	36.8520941826217	38.0	35.0	41.0	31.0	41.0
24	36.73107989491882	38.0	35.0	41.0	31.0	41.0
25	36.59661311072062	38.0	35.0	40.0	31.0	41.0
26	36.4527465283279	38.0	35.0	40.0	31.0	41.0
27	36.41090374667234	38.0	35.0	40.0	31.0	41.0
28	36.41720722278012	38.0	35.0	40.0	31.0	41.0
29	36.33779794496918	38.0	35.0	40.0	30.0	41.0
30	36.31204550792881	38.0	35.0	40.0	30.0	41.0
31	36.13722387882439	38.0	35.0	41.0	30.0	41.0
32	35.809239248160395	38.0	35.0	41.0	29.0	41.0
33	35.48525514545069	38.0	35.0	41.0	26.0	41.0
34	35.06459963105708	38.0	34.0	40.0	24.0	41.0
35	34.83186434940573	38.0	34.0	40.0	23.0	41.0
36	34.65036539677953	38.0	34.0	40.0	22.0	41.0
37	34.40139095279641	38.0	33.0	40.0	21.0	41.0
38	34.44678396259506	38.0	34.0	40.0	21.0	41.0
39	34.3109210806907	38.0	33.0	40.0	21.0	41.0
40	34.171880856391866	38.0	33.0	40.0	20.0	41.0
41	34.079159725646484	37.0	33.0	40.0	19.0	41.0
42	34.04782770610833	37.0	33.0	40.0	19.0	41.0
43	33.97418120804885	37.0	33.0	40.0	19.0	41.0
44	33.80919981988008	37.0	33.0	40.0	18.0	41.0
45	33.75016077842965	37.0	33.0	40.0	19.0	41.0
46	33.57284194476627	36.0	33.0	40.0	18.0	41.0
47	33.59387212398944	36.0	33.0	40.0	18.0	41.0
48	33.41959960581336	36.0	33.0	40.0	18.0	41.0
49	33.38435961861313	36.0	33.0	40.0	18.0	41.0
50	33.252878564983696	36.0	32.0	40.0	18.0	41.0
51	32.57611449203272	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	9.0
13	20.0
14	41.0
15	150.0
16	431.0
17	1011.0
18	2344.0
19	4745.0
20	7990.0
21	12601.0
22	19196.0
23	28066.0
24	41798.0
25	65015.0
26	93241.0
27	105790.0
28	104816.0
29	102691.0
30	108767.0
31	124433.0
32	147356.0
33	184479.0
34	279645.0
35	376570.0
36	329464.0
37	411872.0
38	602442.0
39	1003941.0
40	521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.772029529858624	22.80115813360946	26.655633159279912	18.771179177251998
2	36.29228953532569	23.71000403659041	26.773461209183612	13.224245218900283
3	27.48312217165198	24.28919104949187	34.967138692101436	13.260548086754717
4	23.872693776173826	26.975747520526145	34.616467413608184	14.535091289691838
5	22.2220913288797	30.553046543882832	33.349377117316685	13.87548500992078
6	20.464695941844248	38.85332463346725	30.55968203496086	10.122297389727635
7	83.52691256610548	3.29868052298248	11.642594178895244	1.5318127320167974
8	83.82731278719234	2.7036981563191875	11.518154679547854	1.9508343769406107
9	78.02714829433019	4.552475795483587	14.071881120849843	3.3484947893363817
10	35.92721731786238	25.278528344245434	24.04567333525506	14.748581002637126
11	30.283299406580337	24.472460428070917	28.104454169552668	17.139785995796082
12	26.899367248225786	22.42218985149723	31.9667186847495	18.711724215527482
13	23.852354553521607	25.105717076604584	32.88183945429336	18.160088915580445
14	21.12314822316695	26.836979207111707	33.748949080058885	18.290923489662458
15	21.260762538133037	22.918048559773872	38.06326844576364	17.757920456329455
16	24.00564401407782	21.89849093065407	36.12426255291864	17.97160250234947
17	23.203446801032154	22.421684977176074	32.83289068677573	21.541977535016038
18	24.741269941634126	22.342972666344668	33.81931894377407	19.096438448247135
19	26.178526925789004	24.836643105063626	30.288444316329244	18.696385652818122
20	27.44446322363216	24.439884013539288	29.582437682280666	18.53321508054789
21	25.364933978065856	25.091364220903188	31.396066452038983	18.14763534899197
22	24.15919793261178	22.64330076252852	31.760465503740757	21.437035801118945
23	22.849289485559513	25.852979155181778	30.99565303209486	20.30207832716385
24	22.72751860762394	24.51850015783333	33.880913610954906	18.873067623587826
25	22.99856399318083	24.846428050240284	31.755440802163555	20.39956715441533
26	21.39270302739472	26.09983865659194	31.129949601521933	21.37750871449141
27	21.267349945942385	24.926318401154383	32.26923456965835	21.53709708324488
28	20.369515111489473	25.952872145867328	33.34841545194306	20.329197290700144
29	22.053319055808085	24.90927288240684	32.44673395599563	20.590674105789443
30	23.22566127116295	24.61598898508481	33.08895813413837	19.069391609613863
31	25.06162471922376	24.97538737684372	30.16412502515356	19.798862878778955
32	25.70764747559233	24.92901106420054	29.69889535902695	19.66444610118018
33	24.753531175147874	25.544765402934182	29.059267677393002	20.64243574452494
34	22.00718315950831	27.118843328121912	30.751390027193494	20.12258348517629
35	22.06923461774162	27.368275284406522	30.063967576490263	20.498522521361593
36	24.17783019922581	29.074221573952908	27.187410069261542	19.560538157559737
37	22.74326587811709	28.54073770793309	28.40514289025162	20.3108535236982
38	23.694593348978028	28.660104422434596	27.649394114752162	19.995908113835213
39	22.808635081889413	27.70601216362448	27.269007376213832	22.216345378272276
40	24.423703993628003	26.056515631510024	28.222210094553347	21.297570280308626
41	21.189455050678564	26.459621714500305	28.50082859492755	23.850094639893584
42	22.796253640203958	27.327140048049607	27.476270305864887	22.400336005881545
43	22.51554351764211	26.078657976737794	28.67813564819011	22.727662857429983
44	22.86433954865678	27.087973869628467	28.383481377710662	21.664205204004087
45	21.851465493883687	27.57464867358697	27.75017664590832	22.823709186621024
46	22.85342464666611	27.46134045093932	27.902504441090905	21.782730461303668
47	22.066277496717714	27.27811915562895	29.19825236551651	21.457350982136823
48	22.523333007168496	26.732061514848954	29.10396107563234	21.640644402350215
49	22.63500639868098	25.907241123888706	29.269367519896257	22.188384957534062
50	21.78662520606686	27.27460907701521	29.21407176091268	21.72469395600525
51	21.548877484071816	27.464610113209652	28.589109476226547	22.39740292649198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2117.0
1	3277.5
2	4438.0
3	45451.0
4	86464.0
5	60717.5
6	34971.0
7	35617.5
8	36264.0
9	37452.0
10	38640.0
11	39174.0
12	39708.0
13	39193.5
14	38679.0
15	37629.5
16	36580.0
17	34663.0
18	32746.0
19	31120.0
20	29494.0
21	28694.5
22	27895.0
23	28424.0
24	28953.0
25	29830.5
26	33205.5
27	35703.0
28	40889.5
29	46076.0
30	51500.0
31	56924.0
32	65002.5
33	73081.0
34	86139.5
35	99198.0
36	109517.5
37	119837.0
38	133554.0
39	147271.0
40	166678.0
41	186085.0
42	203789.5
43	221494.0
44	237253.0
45	253012.0
46	270031.5
47	287051.0
48	297071.0
49	307091.0
50	308340.5
51	309590.0
52	296741.5
53	283893.0
54	267076.0
55	250259.0
56	234409.0
57	218559.0
58	204420.5
59	190282.0
60	178905.5
61	167529.0
62	149933.0
63	132337.0
64	117164.0
65	101991.0
66	85502.0
67	69013.0
68	57343.0
69	45673.0
70	38568.0
71	31463.0
72	26964.0
73	22465.0
74	19433.5
75	13555.5
76	10709.0
77	7708.0
78	4707.0
79	3438.0
80	2169.0
81	1623.5
82	1078.0
83	784.0
84	490.0
85	360.5
86	231.0
87	146.5
88	62.0
89	51.0
90	40.0
91	29.5
92	19.0
93	12.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4159451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.654252734960124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14505093713737	19.790986339926945
2	6.072942451708227	3.1159360100198246
3	2.6026661571959737	2.003083661442989
4	1.5654995815650212	1.6064688768777349
5	1.1163087830793996	1.4319033825687348
6	0.8880537195133742	1.3669412737570492
7	0.7459720877060965	1.3396149529866626
8	0.6212484544878156	1.2750131890107037
9	0.5874339420916528	1.3563160933961875
>10	7.594407952405792	45.07918936522612
>50	0.9657470218695131	16.071549907619467
>100	0.09030481707008922	3.406523687517592
>500	0.003415378046495505	0.5907222386443178
>1k	8.538445109085898E-4	0.30944100107360295
>5k	0.0	0.0
>10k+	9.487161232317665E-5	1.2563100199321406
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51618	1.240981081397521	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026686214118161265	0.0
2	0.0	0.0	0.0	0.08152518204926563	0.0
3	0.0	0.0	0.0	0.14797625936692127	0.0
4	0.0	0.0	0.0	0.2359445994194907	0.0
5	0.0	0.0	0.0	0.40341862423670816	0.0
6	0.0	0.0	0.0	0.8521557292056091	0.0
7	0.0	0.0	0.0	1.1320724778342142	0.0
8	0.0	0.0	0.0	1.7898756350297191	0.0
9	0.0	0.0	0.0	2.2091136546625987	0.0
10	0.0	0.0	0.0	2.755387670151662	0.0
11	0.0	0.0	0.0	3.0560763908506194	0.0
12	0.0	0.0	0.0	3.3155577502896416	0.0
13	2.4041634340685826E-5	0.0	0.0	3.4894268498414815	0.0
14	2.4041634340685826E-5	0.0	0.0	3.6045862783333664	0.0
15	2.4041634340685826E-5	0.0	0.0	3.681783966201309	0.0
16	2.4041634340685826E-5	0.0	0.0	3.7892500717041746	0.0
17	2.4041634340685826E-5	0.0	0.0	3.8951534709748956	0.0
18	2.4041634340685826E-5	0.0	0.0	4.059742499671231	0.0
19	2.4041634340685826E-5	0.0	0.0	4.145715384073523	0.0
20	1.6829144038480078E-4	0.0	0.0	4.242531045563465	0.0
21	1.6829144038480078E-4	0.0	0.0	4.348410403199845	0.0
22	2.1637470906617245E-4	0.0	0.0	4.462391791609037	0.0
23	2.1637470906617245E-4	0.0	0.0	4.593154240788027	0.0
24	2.1637470906617245E-4	0.0	0.0	4.702447510500785	0.0
25	2.1637470906617245E-4	0.0	0.0	4.808639409383594	0.0
26	2.1637470906617245E-4	0.0	0.0	4.9098066066892	0.0
27	2.1637470906617245E-4	0.0	0.0	5.022273372134928	0.0
28	2.1637470906617245E-4	0.0	0.0	5.131758974922412	0.0
29	2.1637470906617245E-4	0.0	0.0	5.252904770365127	0.0
30	2.1637470906617245E-4	0.0	0.0	5.416243634075747	0.0
31	2.4041634340685826E-4	0.0	0.0	5.547054166523418	0.0
32	2.6445797774754407E-4	0.0	0.0	5.694020677248031	0.0
33	2.6445797774754407E-4	0.0	0.0	5.833606406230053	0.0
34	2.6445797774754407E-4	0.0	0.0	5.976606047288453	0.0
35	2.6445797774754407E-4	0.0	0.0	6.1321313798383486	0.0
36	2.6445797774754407E-4	0.0	0.0	6.275082937628067	0.0
37	2.6445797774754407E-4	0.0	0.0	6.425727818406804	0.0
38	2.6445797774754407E-4	0.0	0.0	6.607626823828433	0.0
39	2.6445797774754407E-4	0.0	0.0	6.912618997074373	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	38665	0.0	42.951633	1
AGGGCGA	5675	0.0	37.823788	6
GGCGATA	2315	0.0	37.807777	8
GGGCGAT	11415	0.0	37.687252	7
GTTTTTT	45060	0.0	37.669773	2
CGTTCGG	1110	0.0	37.5	45
ACACGAC	2235	0.0	36.744965	26
ATAGGGC	2265	0.0	36.65563	4
CACGACC	2260	0.0	36.438053	27
CATACGA	595	0.0	36.302525	18
ACGACCA	2290	0.0	35.960697	28
TATAGCG	245	0.0	35.816326	1
GACACGA	2305	0.0	35.72668	25
CGTAAGG	755	0.0	35.463577	2
AACACGT	2150	0.0	35.372093	41
ATACTAT	2605	0.0	35.326294	45
TAAGGGA	3720	0.0	35.322582	4
GACCGAT	3030	0.0	35.272274	9
GCGAGAC	2355	0.0	35.063694	21
TAGGGCG	2405	0.0	34.89605	5
>>END_MODULE