FastQCFastQC Report
Thu 26 May 2016
SRR1547672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547672_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3094714
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190920.61692292082564No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33510.10828141146483972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114300.040.6102371
TACGGGA6400.038.6718754
TATCACG1050.038.571431
CATACGA4350.038.27586418
GCGTAAG2000.038.251
ATAGGGT16000.037.68754
AGGGTAC15550.037.620586
ATAGGGC14250.037.421054
GGGCGAT84550.037.1230057
GTAGCAT15950.036.67711629
TAGGGTA14900.036.5436255
CATGCAC31500.036.545
TCGATAG2350.036.382981
GGCGATA16750.036.1343278
GGTACCT17350.036.0518768
TAGGGAC23150.035.9611245
TGATTCG4700.035.90425515
AACACGT7150.035.87412641
AGGGCGA43750.035.742866
GTAGGGA27300.035.6868134
Produced by FastQC (version 0.11.5)