##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547672_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3094714
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44822267905855	31.0	31.0	33.0	30.0	34.0
2	31.875475084288887	31.0	31.0	34.0	30.0	34.0
3	32.02471795455089	33.0	31.0	34.0	30.0	34.0
4	35.72754154341887	37.0	35.0	37.0	33.0	37.0
5	35.619854371033966	37.0	35.0	37.0	33.0	37.0
6	35.66591323140038	37.0	35.0	37.0	33.0	37.0
7	36.01926575444451	37.0	35.0	37.0	35.0	37.0
8	36.024464619347704	37.0	35.0	37.0	35.0	37.0
9	37.775457764433156	39.0	38.0	39.0	35.0	39.0
10	37.14553590412555	39.0	37.0	39.0	33.0	39.0
11	36.92824312682852	39.0	37.0	39.0	33.0	39.0
12	36.461857218469945	39.0	35.0	39.0	32.0	39.0
13	36.284973991134564	39.0	35.0	39.0	32.0	39.0
14	37.370276865648975	39.0	35.0	41.0	32.0	41.0
15	37.51788598235572	39.0	35.0	41.0	32.0	41.0
16	37.56618479122788	39.0	35.0	41.0	33.0	41.0
17	37.49705239320984	39.0	35.0	41.0	32.0	41.0
18	37.37956690020467	39.0	35.0	41.0	32.0	41.0
19	37.32873990940681	39.0	35.0	41.0	32.0	41.0
20	37.22403201071246	39.0	35.0	41.0	32.0	41.0
21	37.12418627375583	39.0	35.0	41.0	32.0	41.0
22	37.0230619049127	39.0	35.0	41.0	32.0	41.0
23	36.912788710039116	39.0	35.0	41.0	31.0	41.0
24	36.787144143206774	38.0	35.0	41.0	31.0	41.0
25	36.63820566294656	38.0	35.0	41.0	31.0	41.0
26	36.47852531768687	38.0	35.0	40.0	31.0	41.0
27	36.430708621216695	38.0	35.0	40.0	30.0	41.0
28	36.434286334698456	38.0	35.0	40.0	30.0	41.0
29	36.42139499805152	38.0	35.0	41.0	30.0	41.0
30	36.437554488072244	38.0	35.0	41.0	31.0	41.0
31	36.317282307832	38.0	35.0	41.0	30.0	41.0
32	36.1346983921616	38.0	35.0	41.0	30.0	41.0
33	36.00136167671714	38.0	35.0	41.0	30.0	41.0
34	35.78872845762161	38.0	35.0	40.0	29.0	41.0
35	35.65303966699346	38.0	35.0	40.0	28.0	41.0
36	35.486474679081816	38.0	35.0	40.0	27.0	41.0
37	35.24231512184971	38.0	34.0	40.0	26.0	41.0
38	35.297210016822234	38.0	34.0	40.0	27.0	41.0
39	35.1654055915991	38.0	34.0	40.0	26.0	41.0
40	35.01485048376038	37.0	34.0	40.0	26.0	41.0
41	34.939583431619205	37.0	34.0	40.0	25.0	41.0
42	34.875158092153264	37.0	34.0	40.0	26.0	41.0
43	34.789909826885456	37.0	34.0	40.0	25.0	41.0
44	34.62236671950946	37.0	34.0	40.0	25.0	41.0
45	34.549945810824525	36.0	34.0	40.0	24.0	41.0
46	34.36782849723755	36.0	33.0	40.0	24.0	41.0
47	34.37577624297431	36.0	33.0	40.0	24.0	41.0
48	34.19852561496798	36.0	33.0	40.0	23.0	41.0
49	34.16435088993684	36.0	33.0	40.0	24.0	41.0
50	34.026785027630986	35.0	33.0	40.0	24.0	41.0
51	33.31430594232617	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	8.0
13	14.0
14	53.0
15	104.0
16	239.0
17	649.0
18	1452.0
19	2826.0
20	4828.0
21	7856.0
22	11517.0
23	16661.0
24	23876.0
25	33251.0
26	44092.0
27	52120.0
28	58413.0
29	65553.0
30	76662.0
31	91627.0
32	114156.0
33	147240.0
34	239285.0
35	342574.0
36	255394.0
37	326894.0
38	458498.0
39	718494.0
40	374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.404868430491476	23.83881030686519	28.89498027927621	14.861340983367121
2	31.715693275695266	26.227496304989735	28.753804067193283	13.303006352121717
3	28.401332077859216	25.65687814770606	31.91092941060143	14.030860363833298
4	25.5191917572997	28.450221894494938	30.88569735361652	15.144888994588838
5	22.879594043262156	32.169467033141025	29.97721275697851	14.973726166618304
6	21.917534221255988	40.661915769922516	26.592925872956275	10.82762413586522
7	88.1125040956935	3.8790660461677553	6.429964125925691	1.5784657322130575
8	88.38558264188549	3.399861828912139	6.305396879970168	1.909158649232207
9	82.86277827288725	4.984854820186938	8.839201296145621	3.313165610780188
10	44.25904946305216	22.41728961060699	19.433233571826023	13.890427354514829
11	37.01314564124504	23.727006760560105	22.0464960574709	17.213351540723956
12	31.53700148058916	21.92619414912008	27.625945402386133	18.910858967904627
13	23.94841655804058	27.77587848182417	28.97999621289722	19.295708747238034
14	21.618799023108437	29.591975219681043	29.24228216242276	19.54694359478776
15	20.84121505250566	22.16973846371587	37.35181990969117	19.6372265740873
16	23.318471432255127	20.284265363455233	35.19426997131237	21.202993232977263
17	24.13638223112055	21.059522786273625	27.488259011979782	27.31583597062604
18	25.86070958414897	22.562149523348523	30.150637506406085	21.42650338609642
19	28.73990940681433	23.32377079109734	26.249824701087082	21.686495101001256
20	29.98852236426371	23.306741753842196	25.02812860897647	21.67660727291763
21	26.95719216703062	25.04208789568277	27.060788169763022	20.93993176752359
22	26.73743034089742	22.297860157675313	26.539512213406475	24.4251972880208
23	25.187691011188758	25.099799206000945	25.39976230436803	24.312747478442272
24	24.950609329327364	23.725391102376502	30.5128680711691	20.811131497127036
25	23.841039915158557	24.480517424227248	28.690793398032906	22.987649262581293
26	23.08956498080275	26.779663645816704	26.411649024756407	23.71912234862414
27	22.977502929188287	25.620364272756706	28.283324404129107	23.118808393925903
28	21.865025330289004	25.12170753097055	31.28990271798945	21.723364420750997
29	22.75932444807501	23.756379426337944	30.556200023653236	22.928096101933814
30	24.169083152756603	25.09191479406498	29.18411846781318	21.55488358536524
31	27.560769751259727	24.59749107671985	25.871308301833384	21.970430870187034
32	28.47646018339659	24.29145310358243	25.580069757657736	21.652016955363244
33	27.134203677625784	25.323535551265802	25.31600658413023	22.226254186978185
34	24.254551470669018	25.403284439208274	27.44017702443586	22.901987065686846
35	24.473214649237377	25.739309028233304	27.768640333161642	22.018835989367677
36	28.364333505454788	25.217709940240034	25.176284464412547	21.241672089892635
37	25.081057571071184	27.032352585731672	26.546168725122904	21.34042111807424
38	25.49133781021445	27.15717833699657	24.843168060117996	22.508315792670984
39	24.37246220490811	27.297514406824025	25.629541211239555	22.700482177028313
40	25.931540038917973	24.530505888427818	26.053134473815675	23.484819598838534
41	23.070823345872995	23.781066683383344	26.49802211125164	26.65008785949202
42	24.52178133423638	25.390132981593776	25.5318908306228	24.556194853547048
43	24.515318701501982	24.974197938807915	26.671931558134286	23.838551801555813
44	24.02402936103304	26.14464535333475	26.878186481852605	22.95313880377961
45	22.300283644950714	27.714418844520043	25.895737053569405	24.089560456959834
46	24.371395870506934	26.60749910977234	26.58950067760704	22.43160434211368
47	23.95523463557537	25.459250838688163	27.33932764061558	23.24618688512089
48	24.656591853075923	24.528987169735235	28.128673602794958	22.685747374393888
49	23.769563197116113	24.016371141242775	28.42508225315813	23.78898340848298
50	23.402453344638634	26.95215131349779	26.85227132458767	22.793124017275908
51	22.75118153082967	28.07771574368423	25.740763120598544	23.43033960488756
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1932.0
1	2664.0
2	3396.0
3	17073.0
4	30750.0
5	21310.5
6	11871.0
7	12516.5
8	13162.0
9	13948.5
10	14735.0
11	15146.5
12	15558.0
13	15656.5
14	15755.0
15	15440.0
16	15125.0
17	14833.0
18	14541.0
19	13782.0
20	13023.0
21	13343.0
22	13663.0
23	14389.0
24	15115.0
25	16147.0
26	17976.5
27	18774.0
28	22070.0
29	25366.0
30	28395.0
31	31424.0
32	39822.5
33	48221.0
34	56470.0
35	64719.0
36	71621.5
37	78524.0
38	87004.0
39	95484.0
40	114313.0
41	133142.0
42	152873.0
43	172604.0
44	184452.0
45	196300.0
46	196104.0
47	195908.0
48	201823.0
49	207738.0
50	213140.5
51	218543.0
52	216698.5
53	214854.0
54	203945.5
55	193037.0
56	187630.5
57	182224.0
58	176800.5
59	171377.0
60	161941.0
61	152505.0
62	143181.0
63	133857.0
64	120249.0
65	106641.0
66	93440.5
67	80240.0
68	70340.5
69	60441.0
70	53165.0
71	45889.0
72	37042.5
73	28196.0
74	23460.5
75	15504.5
76	12284.0
77	9115.5
78	5947.0
79	4679.5
80	3412.0
81	2391.5
82	1371.0
83	994.5
84	618.0
85	426.5
86	235.0
87	199.0
88	163.0
89	130.5
90	98.0
91	62.5
92	27.0
93	22.5
94	18.0
95	10.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3094714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.785395916254224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62771385582408	15.754658445436814
2	5.942131542503627	2.351348503097933
3	2.0947204898469907	1.2433462267653808
4	1.1374301848834458	0.9001802614006885
5	0.7901993355355617	0.781720335316605
6	0.6118240138867476	0.7263108207492673
7	0.4862395941505472	0.6734310016299143
8	0.42053707526687695	0.6656394025295008
9	0.3629859249192438	0.6463638212899471
>10	5.62015387082023	27.825091606457587
>50	2.3216664429643266	32.522936573382836
>100	0.5814162898121211	14.763069921099921
>500	0.002153218590005765	0.2599066930011494
>1k	6.625287969248508E-4	0.2603327158241244
>5k	0.0	0.0
>10k+	1.656321992312127E-4	0.6256636720184017
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19092	0.61692292082564	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3351	0.10828141146483972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.231316367199037E-5	0.0	0.0	0.016964410927794944	0.0
2	3.231316367199037E-5	0.0	0.0	0.06320454814241316	0.0
3	3.231316367199037E-5	0.0	0.0	0.10307899211364928	0.0
4	3.231316367199037E-5	0.0	0.0	0.1700964935693573	0.0
5	3.231316367199037E-5	0.0	0.0	0.27062274575291934	0.0
6	3.231316367199037E-5	0.0	0.0	0.5452523238011655	0.0
7	3.231316367199037E-5	0.0	0.0	0.6840373617723641	0.0
8	3.231316367199037E-5	0.0	0.0	1.0644925508463787	0.0
9	3.231316367199037E-5	0.0	0.0	1.3004755851429244	0.0
10	3.231316367199037E-5	0.0	0.0	1.6484560447265886	0.0
11	3.231316367199037E-5	0.0	0.0	1.8793659123266317	0.0
12	3.231316367199037E-5	0.0	0.0	2.0715646098476306	0.0
13	3.231316367199037E-5	0.0	0.0	2.1861470882285086	0.0
14	6.462632734398074E-5	0.0	0.0	2.2467342701134903	0.0
15	6.462632734398074E-5	0.0	0.0	2.2998571111902426	0.0
16	6.462632734398074E-5	0.0	0.0	2.3743712666178522	0.0
17	6.462632734398074E-5	0.0	0.0	2.4512442829935175	0.0
18	6.462632734398074E-5	0.0	0.0	2.5638879715540757	0.0
19	6.462632734398074E-5	0.0	0.0	2.626769388059769	0.0
20	1.2925265468796148E-4	0.0	0.0	2.6916542207131258	0.0
21	1.2925265468796148E-4	0.0	0.0	2.768365671270431	0.0
22	1.938789820319422E-4	0.0	0.0	2.8497625305601746	0.0
23	1.938789820319422E-4	0.0	0.0	2.941887360189019	0.0
24	1.938789820319422E-4	0.0	0.0	3.0175647895088207	0.0
25	1.938789820319422E-4	0.0	0.0	3.08526086740164	0.0
26	1.938789820319422E-4	0.0	0.0	3.152827692639772	0.0
27	1.938789820319422E-4	0.0	0.0	3.230863982907629	0.0
28	1.938789820319422E-4	0.0	0.0	3.3028253984051514	0.0
29	1.938789820319422E-4	0.0	0.0	3.3820895888925437	0.0
30	1.938789820319422E-4	0.0	0.0	3.5046211055367316	0.0
31	1.938789820319422E-4	0.0	0.0	3.5878275019921064	0.0
32	1.938789820319422E-4	0.0	0.0	3.676817954744768	0.0
33	1.938789820319422E-4	0.0	0.0	3.7632879807310142	0.0
34	1.938789820319422E-4	0.0	0.0	3.8499518856992925	0.0
35	1.938789820319422E-4	0.0	0.0	3.9504135115555106	0.0
36	1.938789820319422E-4	0.0	0.0	4.040793430346067	0.0
37	1.938789820319422E-4	0.0	0.0	4.139639398018686	0.0
38	1.938789820319422E-4	0.0	0.0	4.250635115231973	0.0
39	1.938789820319422E-4	0.0	0.0	4.417758797743507	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11430	0.0	40.610237	1
TACGGGA	640	0.0	38.671875	4
TATCACG	105	0.0	38.57143	1
CATACGA	435	0.0	38.275864	18
GCGTAAG	200	0.0	38.25	1
ATAGGGT	1600	0.0	37.6875	4
AGGGTAC	1555	0.0	37.62058	6
ATAGGGC	1425	0.0	37.42105	4
GGGCGAT	8455	0.0	37.123005	7
GTAGCAT	1595	0.0	36.677116	29
TAGGGTA	1490	0.0	36.543625	5
CATGCAC	3150	0.0	36.5	45
TCGATAG	235	0.0	36.38298	1
GGCGATA	1675	0.0	36.134327	8
GGTACCT	1735	0.0	36.051876	8
TAGGGAC	2315	0.0	35.961124	5
TGATTCG	470	0.0	35.904255	15
AACACGT	715	0.0	35.874126	41
AGGGCGA	4375	0.0	35.74286	6
GTAGGGA	2730	0.0	35.686813	4
>>END_MODULE