FastQCFastQC Report
Thu 26 May 2016
SRR1547671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547671_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3608218
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525091.4552612951878185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT308000.042.751
ATACTAT29100.038.58247445
ACGTATG950.037.8947371
CATATGC16250.037.833
GTTTTTT357150.037.7614482
CGTTAGG5450.037.568812
TATACTA30200.037.25165644
CACGACC11650.037.08154727
ACGGGTA2750.036.8181845
AGGGCGA47500.036.7105266
GGCGATA20750.036.65068
GGGCGAT94400.036.586337
ACACGAC11900.036.3025226
GTTAACG1250.036.01
GGACTAA35850.035.9623458
GACACGA11950.035.9623425
CCAGTAG17450.035.84527226
TATGGGA24050.035.83164
ATTATAC31900.035.8307242
TAAGGGA31500.035.6428574