##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547671_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3608218
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37495960609919	31.0	31.0	33.0	30.0	34.0
2	31.78648961897535	31.0	31.0	34.0	30.0	34.0
3	31.87312850831075	31.0	31.0	34.0	30.0	34.0
4	35.61260184390189	37.0	35.0	37.0	33.0	37.0
5	35.50852969526786	37.0	35.0	37.0	33.0	37.0
6	35.55417743606401	37.0	35.0	37.0	33.0	37.0
7	35.95717221076997	37.0	35.0	37.0	35.0	37.0
8	35.96468727776426	37.0	35.0	37.0	35.0	37.0
9	37.69522490049105	39.0	37.0	39.0	35.0	39.0
10	37.116199464666494	39.0	37.0	39.0	33.0	39.0
11	36.85275945078706	39.0	37.0	39.0	32.0	39.0
12	36.44613102645128	39.0	35.0	39.0	32.0	39.0
13	36.31546763527037	39.0	35.0	39.0	32.0	39.0
14	37.37717815276128	40.0	36.0	41.0	32.0	41.0
15	37.491251082944544	40.0	36.0	41.0	32.0	41.0
16	37.54244117179173	40.0	36.0	41.0	32.0	41.0
17	37.4553876733612	39.0	35.0	41.0	32.0	41.0
18	37.285604694616566	39.0	36.0	41.0	32.0	41.0
19	37.150377831938094	39.0	35.0	41.0	32.0	41.0
20	36.95786008495052	38.0	35.0	41.0	32.0	41.0
21	36.853840871033846	38.0	35.0	41.0	31.0	41.0
22	36.7648526225411	38.0	35.0	41.0	31.0	41.0
23	36.63411107643718	38.0	35.0	40.0	31.0	41.0
24	36.487209198557295	38.0	35.0	40.0	31.0	41.0
25	36.33260157784258	38.0	35.0	40.0	30.0	41.0
26	36.15223941568941	38.0	35.0	40.0	30.0	41.0
27	36.099728176069185	38.0	35.0	40.0	30.0	41.0
28	36.08262970807196	38.0	35.0	40.0	30.0	41.0
29	35.99800926662414	38.0	35.0	40.0	30.0	41.0
30	35.97559570957187	38.0	35.0	40.0	30.0	41.0
31	35.79819207154335	38.0	35.0	40.0	29.0	41.0
32	35.491465870410266	38.0	34.0	40.0	27.0	41.0
33	35.204036729488074	38.0	34.0	40.0	25.0	41.0
34	34.82786655351755	38.0	34.0	40.0	23.0	41.0
35	34.58612423085302	37.0	34.0	40.0	23.0	41.0
36	34.376120012704334	37.0	33.0	40.0	22.0	41.0
37	34.10684332265955	37.0	33.0	40.0	21.0	41.0
38	34.144328031177714	37.0	33.0	40.0	21.0	41.0
39	33.9893094042544	37.0	33.0	40.0	21.0	41.0
40	33.81422962803245	36.0	33.0	40.0	20.0	41.0
41	33.69253853287135	36.0	33.0	40.0	20.0	41.0
42	33.624169881088115	36.0	33.0	40.0	19.0	41.0
43	33.51076431634674	36.0	33.0	40.0	18.0	41.0
44	33.322061471895545	35.0	33.0	40.0	18.0	41.0
45	33.24905950804524	35.0	33.0	40.0	19.0	41.0
46	33.04529410362677	35.0	32.0	40.0	18.0	41.0
47	33.059738629983	35.0	32.0	40.0	18.0	41.0
48	32.86274498935486	35.0	32.0	39.0	18.0	41.0
49	32.81839595057727	35.0	32.0	39.0	18.0	41.0
50	32.670815344305694	35.0	32.0	39.0	18.0	41.0
51	31.98795111603567	35.0	31.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	8.0
13	18.0
14	49.0
15	172.0
16	461.0
17	1163.0
18	2478.0
19	4733.0
20	8031.0
21	12485.0
22	18757.0
23	26838.0
24	39129.0
25	57737.0
26	79845.0
27	92091.0
28	92539.0
29	93090.0
30	101435.0
31	116042.0
32	138681.0
33	174614.0
34	269273.0
35	361133.0
36	313912.0
37	383524.0
38	505150.0
39	714386.0
40	436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.86844586441285	22.828498721529574	26.34716084227727	18.955894571780306
2	35.8131908881337	24.101232242619485	27.043543377922287	13.042033491324526
3	27.33928493234056	24.86875792981466	34.42538671443909	13.36657042340568
4	24.17913773502599	27.024226363262972	34.05839115042384	14.7382447512872
5	22.242392227964054	31.095986994133945	32.292311606449495	14.369309171452501
6	20.848130573041875	38.794025194708304	29.77530736779208	10.582536864457746
7	82.60928247683482	3.9036998318837717	11.604343196558522	1.8826744947228797
8	82.70340095858954	3.3040686566055597	11.442102445029652	2.5504279397752576
9	77.1245528956399	5.328835452846807	13.832645366771077	3.7139662847422192
10	42.98387735995996	25.072404161832797	18.98477309297831	12.95894538522894
11	33.23244327255171	23.197184870758917	26.779673511966294	16.790698344723072
12	28.119005004686525	20.49804086116748	32.148972151904346	19.233981982241648
13	23.285621877613824	24.51736563589007	33.42519770146926	18.771814785026848
14	20.703793396075294	25.808529307264692	34.29429707406814	19.19338022259187
15	19.904340591394423	21.416250348509987	39.143283471231506	19.53612558886409
16	23.354076721528465	19.90888577131426	35.89774786334972	20.83928964380755
17	23.316606701701506	21.19314852927401	31.59834023332293	23.891904535701556
18	25.096876075669485	21.444740866544095	32.61621110476141	20.84217195302501
19	26.916444627237045	24.072132005327838	28.509696476210696	20.501726891224422
20	28.166369105192647	23.204168927708913	28.064795419788936	20.564666547309503
21	26.293145258961626	24.001127426336215	29.414214994770273	20.29151231993189
22	25.820113973157945	21.697608071352672	29.0725227799429	23.409755175546486
23	24.1152835000546	24.51902850659245	28.694219695151457	22.671468298201493
24	23.723344875503642	22.762371896598268	32.70891614641909	20.805367081479
25	24.07227057788637	23.803273527264704	30.035241773085776	22.089214121763153
26	22.6001588595811	25.02795008505584	29.36873548106018	23.003155574302884
27	22.77805831022405	23.613678552681684	30.35925212944451	23.24901100764976
28	21.86001510995178	24.782676656454793	31.294367468927874	22.06294076466555
29	22.78628952020083	23.498524756541872	30.557272315586253	23.157913407671042
30	24.089315002585764	23.67628563462629	30.98720753568659	21.247191827101357
31	26.08836273196353	23.947638418742994	28.199709662775362	21.76428918651811
32	26.67527294636854	23.917235599401145	27.419684730800633	21.98780672342968
33	25.47501287339069	25.22148052030116	27.20442611837755	22.099080487930607
34	22.46868121604626	25.98864591884415	29.086740324448247	22.455932540661347
35	23.57116449172417	26.63927179566201	28.33196331263798	21.45760039997583
36	25.674446499629454	27.822376585893643	25.9919162312255	20.5112606832514
37	24.428180337219093	27.79718409475259	26.074477761598665	21.700157806429658
38	24.217633191786085	27.867800670580323	26.158785306209325	21.755780831424264
39	23.850360482653766	27.405993762017705	24.90866682667178	23.83497892865675
40	25.304624055420156	25.525979860418634	26.519683677649187	22.649712406512023
41	22.437058958189333	24.919724916842608	26.763432808106387	25.879783316861676
42	23.555117789446204	26.093877919793094	25.70798105879412	24.64302323196658
43	23.395121913365546	24.656076766980263	27.82833520591051	24.12046611374368
44	23.4531283863669	25.77327644837424	27.420488451640118	23.353106713618747
45	22.756662707186763	26.56812864411186	26.307944808212806	24.367263840488572
46	23.630224116170364	26.22926330947853	26.839897145904157	23.30061542844695
47	22.96984273123187	26.13849828364029	27.810209915254564	23.081449069873273
48	23.523412388054158	25.066473256327637	28.08408471993655	23.326029635681657
49	23.720046848610586	24.58773278111245	27.9403295477158	23.751890822561165
50	22.272601045723956	26.006604922429855	28.53436239162933	23.186431640216863
51	22.176320832056156	26.29383812175428	27.773238756638317	23.75660228955124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2669.0
1	3674.5
2	4680.0
3	41331.0
4	77982.0
5	52803.5
6	27625.0
7	28432.0
8	29239.0
9	31083.5
10	32928.0
11	33618.0
12	34308.0
13	34566.0
14	34824.0
15	33710.5
16	32597.0
17	31208.5
18	29820.0
19	28052.0
20	26284.0
21	25429.5
22	24575.0
23	23376.5
24	22178.0
25	23006.5
26	24157.0
27	24479.0
28	27238.5
29	29998.0
30	33461.5
31	36925.0
32	41952.0
33	46979.0
34	56780.5
35	66582.0
36	74143.0
37	81704.0
38	87758.0
39	93812.0
40	110071.0
41	126330.0
42	151382.5
43	176435.0
44	193567.5
45	210700.0
46	221435.5
47	232171.0
48	227886.5
49	223602.0
50	216938.0
51	210274.0
52	205070.5
53	199867.0
54	203662.5
55	207458.0
56	207978.5
57	208499.0
58	206041.0
59	203583.0
60	195290.0
61	186997.0
62	176528.0
63	166059.0
64	149877.5
65	133696.0
66	119070.5
67	104445.0
68	91800.5
69	79156.0
70	67771.5
71	56387.0
72	48083.5
73	39780.0
74	33094.0
75	21222.0
76	16036.0
77	12231.0
78	8426.0
79	6353.5
80	4281.0
81	3024.0
82	1767.0
83	1446.5
84	1126.0
85	789.0
86	452.0
87	317.5
88	183.0
89	112.5
90	42.0
91	29.5
92	17.0
93	15.0
94	13.0
95	7.0
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3608218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.282235034360824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.01276705465207	20.766321144455674
2	6.169704602728272	3.2430725292296443
3	2.456624390651555	1.9369673887874281
4	1.5148094900969682	1.5925031620403516
5	1.0170991317953564	1.3365819217544948
6	0.757617124795055	1.1947122803952188
7	0.6213124244927493	1.1430635419200899
8	0.5117384292957732	1.075970373989292
9	0.4569867952400989	1.0809570924089644
>10	6.147210006858315	38.69732319660383
>50	1.2135551525635206	21.17434586742941
>100	0.1167184012876112	4.315829136616908
>500	0.002464191596738465	0.45202779128955306
>1k	0.0012856651809028637	0.511751889296444
>5k	0.0	0.0
>10k+	1.0713876507523867E-4	1.4785726837827105
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52509	1.4552612951878185	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.77145117063326E-5	0.0	0.0	0.03852317127180231	0.0
2	2.77145117063326E-5	0.0	0.0	0.11404521567155865	0.0
3	2.77145117063326E-5	0.0	0.0	0.20287022569035462	0.0
4	2.77145117063326E-5	0.0	0.0	0.3350130175061485	0.0
5	2.77145117063326E-5	0.0	0.0	0.578374144799455	0.0
6	2.77145117063326E-5	0.0	0.0	1.220574810058594	0.0
7	2.77145117063326E-5	0.0	0.0	1.6120700024222483	0.0
8	2.77145117063326E-5	0.0	0.0	2.4778159191046663	0.0
9	2.77145117063326E-5	0.0	0.0	3.0083825312106973	0.0
10	2.77145117063326E-5	0.0	0.0	3.67874114036347	0.0
11	2.77145117063326E-5	0.0	0.0	4.075668377021565	0.0
12	2.77145117063326E-5	0.0	0.0	4.409351097965811	0.0
13	2.77145117063326E-5	0.0	0.0	4.639409259640077	0.0
14	2.77145117063326E-5	0.0	0.0	4.790065345275702	0.0
15	2.77145117063326E-5	0.0	0.0	4.888701292438539	0.0
16	2.77145117063326E-5	0.0	0.0	5.013998599862869	0.0
17	2.77145117063326E-5	0.0	0.0	5.146446251307432	0.0
18	2.77145117063326E-5	0.0	0.0	5.352947078031316	0.0
19	2.77145117063326E-5	0.0	0.0	5.456433064742762	0.0
20	1.940015819443282E-4	0.0	0.0	5.572778584885946	0.0
21	1.940015819443282E-4	0.0	0.0	5.697881890728332	0.0
22	2.217160936506608E-4	0.0	0.0	5.838255892520906	0.0
23	2.217160936506608E-4	0.0	0.0	5.991239997139862	0.0
24	2.217160936506608E-4	0.0	0.0	6.121304200577681	0.0
25	2.217160936506608E-4	0.0	0.0	6.241363465289514	0.0
26	2.217160936506608E-4	0.0	0.0	6.3564064033825005	2.77145117063326E-5
27	2.217160936506608E-4	0.0	0.0	6.482507431646314	2.77145117063326E-5
28	2.217160936506608E-4	0.0	0.0	6.603924707431757	2.77145117063326E-5
29	2.217160936506608E-4	0.0	0.0	6.741333256471754	2.77145117063326E-5
30	2.217160936506608E-4	0.0	0.0	6.932147669569854	2.77145117063326E-5
31	2.217160936506608E-4	0.0	0.0	7.075293122533062	2.77145117063326E-5
32	2.217160936506608E-4	0.0	0.0	7.2401390381623285	2.77145117063326E-5
33	2.217160936506608E-4	0.0	0.0	7.386499374483471	2.77145117063326E-5
34	2.217160936506608E-4	0.0	0.0	7.542338073808179	2.77145117063326E-5
35	2.217160936506608E-4	0.0	0.0	7.71289317884895	2.77145117063326E-5
36	2.217160936506608E-4	0.0	0.0	7.868454733056595	2.77145117063326E-5
37	2.217160936506608E-4	0.0	0.0	8.022076271444796	2.77145117063326E-5
38	2.217160936506608E-4	0.0	0.0	8.219043306141701	2.77145117063326E-5
39	2.217160936506608E-4	0.0	0.0	8.532937865727625	2.77145117063326E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	30800	0.0	42.75	1
ATACTAT	2910	0.0	38.582474	45
ACGTATG	95	0.0	37.894737	1
CATATGC	1625	0.0	37.8	33
GTTTTTT	35715	0.0	37.761448	2
CGTTAGG	545	0.0	37.56881	2
TATACTA	3020	0.0	37.251656	44
CACGACC	1165	0.0	37.081547	27
ACGGGTA	275	0.0	36.818184	5
AGGGCGA	4750	0.0	36.710526	6
GGCGATA	2075	0.0	36.6506	8
GGGCGAT	9440	0.0	36.58633	7
ACACGAC	1190	0.0	36.30252	26
GTTAACG	125	0.0	36.0	1
GGACTAA	3585	0.0	35.962345	8
GACACGA	1195	0.0	35.96234	25
CCAGTAG	1745	0.0	35.845272	26
TATGGGA	2405	0.0	35.8316	4
ATTATAC	3190	0.0	35.83072	42
TAAGGGA	3150	0.0	35.642857	4
>>END_MODULE