FastQCFastQC Report
Thu 26 May 2016
SRR1547670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547670_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2488427
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154880.6224012197263573No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34720.1395258932651028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101450.040.9635281
ACGCTAG501.0822987E-940.51
GCTACGA1950.040.38461710
ACCCGCT2950.039.6610234
TATGGGC10950.039.2465744
TATTACG700.038.5714261
CGCGTAA356.2512263E-638.57142631
GACCGAT18150.038.4297529
GCGATCT2450.037.653069
AGGGTAC11700.037.56
GTAGGGC12100.037.1900834
GGGCGAT54300.037.002767
TAACGGG5900.036.9915283
GGCGATT15650.036.805118
AGGGCGA27900.036.61296
CCGACGG800.036.56252
GGCACCG14800.036.3344578
GGTACCT12550.036.2151378
TAGCATA10850.036.0829530
ATAGGGC13850.036.0649834
Produced by FastQC (version 0.11.5)