##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547670_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2488427
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.370522422397762	31.0	31.0	33.0	30.0	34.0
2	31.785558507442655	31.0	31.0	34.0	30.0	34.0
3	31.919946616878857	31.0	31.0	34.0	30.0	34.0
4	35.62872650071712	37.0	35.0	37.0	33.0	37.0
5	35.50117885716559	37.0	35.0	37.0	33.0	37.0
6	35.55058717816516	37.0	35.0	37.0	33.0	37.0
7	35.96267079564721	37.0	35.0	37.0	35.0	37.0
8	35.97438261198741	37.0	35.0	37.0	35.0	37.0
9	37.704561154496396	39.0	37.0	39.0	35.0	39.0
10	37.04947583352857	39.0	37.0	39.0	33.0	39.0
11	36.81916568177407	39.0	37.0	39.0	32.0	39.0
12	36.32450379295836	38.0	35.0	39.0	32.0	39.0
13	36.13102936111849	38.0	35.0	39.0	32.0	39.0
14	37.21812494399072	39.0	35.0	41.0	32.0	41.0
15	37.361230608733955	39.0	35.0	41.0	32.0	41.0
16	37.43952344191732	39.0	35.0	41.0	32.0	41.0
17	37.34287081758878	39.0	35.0	41.0	32.0	41.0
18	37.2423547084162	39.0	35.0	41.0	32.0	41.0
19	37.18938148476929	39.0	35.0	41.0	32.0	41.0
20	37.074863357454326	39.0	35.0	41.0	32.0	41.0
21	36.9739735182105	39.0	35.0	41.0	32.0	41.0
22	36.861426917486426	38.0	35.0	41.0	31.0	41.0
23	36.752646953276106	38.0	35.0	41.0	31.0	41.0
24	36.631871057499374	38.0	35.0	40.0	31.0	41.0
25	36.48971900722826	38.0	35.0	40.0	31.0	41.0
26	36.31128700982589	38.0	35.0	40.0	30.0	41.0
27	36.295037387072234	38.0	35.0	40.0	30.0	41.0
28	36.295700456553476	38.0	35.0	40.0	30.0	41.0
29	36.28726098856828	38.0	35.0	40.0	30.0	41.0
30	36.31068622868985	38.0	35.0	40.0	30.0	41.0
31	36.204554925661874	38.0	35.0	40.0	30.0	41.0
32	35.97935282007469	38.0	35.0	40.0	30.0	41.0
33	35.862600349538084	38.0	35.0	40.0	29.0	41.0
34	35.671275066538016	38.0	35.0	40.0	29.0	41.0
35	35.54797187138703	38.0	35.0	40.0	28.0	41.0
36	35.3906041848927	38.0	34.0	40.0	27.0	41.0
37	35.10945227647827	38.0	34.0	40.0	26.0	41.0
38	35.1871833089739	38.0	34.0	40.0	26.0	41.0
39	35.05465822384985	37.0	34.0	40.0	26.0	41.0
40	34.9195925779619	37.0	34.0	40.0	25.0	41.0
41	34.86008952643578	37.0	34.0	40.0	25.0	41.0
42	34.8183736151392	37.0	34.0	40.0	25.0	41.0
43	34.73638125611079	37.0	34.0	40.0	25.0	41.0
44	34.570263865486105	37.0	34.0	40.0	24.0	41.0
45	34.50191828010225	36.0	34.0	40.0	24.0	41.0
46	34.313754432016694	36.0	33.0	40.0	23.0	41.0
47	34.33095606180129	36.0	33.0	40.0	23.0	41.0
48	34.15601462289229	36.0	33.0	40.0	23.0	41.0
49	34.12096235895206	36.0	33.0	40.0	24.0	41.0
50	33.974080413048085	35.0	33.0	40.0	23.0	41.0
51	33.266999996383255	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	9.0
13	13.0
14	24.0
15	91.0
16	194.0
17	552.0
18	1254.0
19	2485.0
20	4172.0
21	6578.0
22	10068.0
23	14290.0
24	21162.0
25	29730.0
26	38267.0
27	44277.0
28	48529.0
29	54578.0
30	63714.0
31	76937.0
32	93818.0
33	120783.0
34	194107.0
35	285886.0
36	197823.0
37	248777.0
38	349816.0
39	580096.0
40	390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.25341671666479	23.300542873068007	28.563586554879848	14.882453855387359
2	31.318579970398968	26.012055005029282	28.324037635020034	14.345327389551713
3	27.39995989434289	25.944301359855043	32.36377840298309	14.291960342818976
4	25.26387151401267	29.1062988787696	30.81424530436296	14.815584302854775
5	23.18886589801509	32.78107012984508	29.91484178559387	14.115222186545958
6	21.096218615213548	41.20972807319644	27.082771566133946	10.611281745456065
7	86.86190111263059	4.163031505445006	7.16014574669058	1.8149216352338244
8	87.19608813117684	3.647846611534114	6.544174291630818	2.6118909656582248
9	81.5791662765273	5.5088616222215885	9.435800206315074	3.476171894936038
10	44.24276862451661	23.95682091538148	16.999092197601133	14.801318262500768
11	36.236264917556355	26.18602032528983	21.2550739885076	16.322640768646217
12	31.051222318356135	22.393463822728172	27.55314903752451	19.002164821391183
13	22.078204423919207	31.324125642423912	28.823228489322773	17.77444144433411
14	19.917200705505927	32.26922067635498	29.839573352965548	17.974005265173542
15	18.997262125832908	24.42133122651378	39.01725869394602	17.5641479537073
16	20.585775672744266	21.407740713310055	37.64084700897394	20.365636604971733
17	20.50279152251603	23.414631009870895	28.807475565889618	27.27510190172346
18	24.199825833749593	24.01665791280998	30.436577002258858	21.34693925118157
19	27.671617451506513	26.51494297401531	25.553210924009424	20.26022865046875
20	28.49297970163481	24.70424087184394	25.42260632921922	21.38017309730203
21	25.00981543762385	26.78173802164982	26.68561303988423	21.522833500842097
22	24.818690682909324	24.663210936065234	26.30243925178436	24.215659129241082
23	23.496851625544974	28.45697301950188	25.049237932235908	22.99693742271724
24	23.025389131366925	24.896129161112622	31.646337224278632	20.432144483241824
25	21.846009547396807	26.23842290732258	29.53134650926067	22.384221036019945
26	21.01926236936024	29.21580580824754	27.704328879247814	22.060602943144403
27	22.11324664135215	27.92430720290368	27.537154997916353	22.425291157827818
28	20.631989606285416	27.90107967804561	31.72538314364858	19.741547572020394
29	21.116311629796655	25.42835293138999	30.458197085950285	22.99713835286307
30	22.0878490709191	26.846879574928256	28.988151953020925	22.07711940113172
31	26.228657702235186	26.044083270274754	25.16404941756379	22.563209609926272
32	26.437866170074507	26.02640141744162	26.14535206377362	21.39038034871025
33	24.913650269829095	27.609128176153046	24.65031122070288	22.82691033331498
34	21.300122527202927	27.628859516473657	27.461926751317193	23.609091205006212
35	22.0609646174069	26.866048310840547	28.138257622184614	22.93472944956794
36	26.360909924221204	26.61066609548924	25.94940498555915	21.079018994730404
37	22.800106251861116	29.136317842556764	27.436488994854987	20.627086910727137
38	23.341532622817546	28.719066301723938	25.669710222562287	22.269690852896225
39	22.748587762470027	28.712797281174012	26.513496276965327	22.025118679390634
40	24.297317140506834	26.715873119846396	25.98030804198797	23.006501697658802
41	20.828258172733214	25.651546137379157	27.514048031145784	26.006147658741845
42	23.549575695811047	27.136058240808346	25.008087438369703	24.3062786250109
43	23.745000355646358	25.958205725946552	27.04113883991775	23.255655078489344
44	22.746578461011715	28.499972070709727	26.6707843951219	22.082665073156658
45	21.189168900675003	29.521902792406607	25.552005343134436	23.73692296378395
46	23.381638279925433	28.22373330622116	26.468929970619993	21.925698443233415
47	23.231422902902114	26.656679098884556	27.252517353332046	22.859380644881284
48	24.10185229464236	24.850960064329797	27.81737217929238	23.229815461735466
49	22.25285290667558	25.47995179283941	28.090235317330986	24.176959983154017
50	21.216696330653864	27.970400578357328	27.850485467325342	22.962417623663463
51	21.252542268670126	29.16199671519398	25.801962444548305	23.783498571587593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1562.0
1	2019.5
2	2477.0
3	13408.5
4	24340.0
5	16970.5
6	9601.0
7	9971.0
8	10341.0
9	11220.5
10	12100.0
11	12523.5
12	12947.0
13	13126.5
14	13306.0
15	13361.5
16	13417.0
17	12884.0
18	12351.0
19	12519.0
20	12687.0
21	12715.5
22	12744.0
23	13145.5
24	13547.0
25	14556.5
26	17342.0
27	19118.0
28	22538.0
29	25958.0
30	29361.5
31	32765.0
32	39211.0
33	45657.0
34	50235.5
35	54814.0
36	62340.0
37	69866.0
38	78105.5
39	86345.0
40	105929.0
41	125513.0
42	147637.5
43	169762.0
44	177194.0
45	184626.0
46	188354.0
47	192082.0
48	196606.0
49	201130.0
50	191747.5
51	182365.0
52	173373.0
53	164381.0
54	153756.5
55	143132.0
56	133639.0
57	124146.0
58	120603.5
59	117061.0
60	108045.5
61	99030.0
62	91786.0
63	84542.0
64	72506.0
65	60470.0
66	51647.0
67	42824.0
68	38056.5
69	33289.0
70	27993.0
71	22697.0
72	19062.5
73	15428.0
74	13360.5
75	9053.0
76	6813.0
77	5256.5
78	3700.0
79	2518.0
80	1336.0
81	1033.0
82	730.0
83	512.5
84	295.0
85	209.0
86	123.0
87	92.5
88	62.0
89	62.0
90	62.0
91	37.5
92	13.0
93	9.0
94	5.0
95	4.5
96	4.0
97	2.5
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2488427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.03897510111789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7240005954486	18.137202895789308
2	6.789732837142476	3.128569715563361
3	2.3950482488875893	1.6553837091629153
4	1.4131014729571272	1.302256386032491
5	0.950986845910054	1.0954881132206185
6	0.7585189866307425	1.0485300028026512
7	0.5896619150479305	0.9509644325206736
8	0.48100649566826603	0.8865517341741723
9	0.43139720633830464	0.8945054545968016
>10	5.347050072481826	29.072588953476803
>50	1.6932795050358231	27.690478887938113
>100	0.42231864470986413	12.657914224141756
>500	0.0026571639144846453	0.45856612838002814
>1k	0.0010628655657938582	0.38889966512400215
>5k	0.0	0.0
>10k+	1.77144260965643E-4	0.6320996970763506
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15488	0.6224012197263573	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3472	0.1395258932651028	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037935611532908135	0.0
2	0.0	0.0	0.0	0.12369259777361362	0.0
3	0.0	0.0	0.0	0.20398428404771368	0.0
4	0.0	0.0	0.0	0.37734681387077057	0.0
5	0.0	0.0	0.0	0.6067286683515329	0.0
6	0.0	0.0	0.0	1.2642926635983294	0.0
7	0.0	0.0	0.0	1.6015338203612162	0.0
8	0.0	0.0	0.0	2.4938244119678816	0.0
9	0.0	0.0	0.0	2.984656572204047	0.0
10	0.0	0.0	0.0	3.594600122888877	0.0
11	0.0	0.0	0.0	4.007551758600916	0.0
12	0.0	0.0	0.0	4.394985265792406	0.0
13	0.0	0.0	0.0	4.641727484872974	0.0
14	0.0	0.0	0.0	4.769519057621542	0.0
15	0.0	0.0	0.0	4.867532782757943	0.0
16	0.0	0.0	0.0	5.002437282668931	0.0
17	0.0	0.0	0.0	5.153737682479735	0.0
18	0.0	0.0	0.0	5.391156742793741	0.0
19	0.0	0.0	0.0	5.5167782699673324	0.0
20	2.0093014583108125E-4	0.0	0.0	5.645775423590887	0.0
21	2.0093014583108125E-4	0.0	0.0	5.793378708718399	0.0
22	2.813022041635137E-4	0.0	0.0	5.953399476858272	0.0
23	2.813022041635137E-4	0.0	0.0	6.131825446356272	0.0
24	2.813022041635137E-4	0.0	0.0	6.283447334400407	0.0
25	2.813022041635137E-4	0.0	0.0	6.414011743161443	0.0
26	2.813022041635137E-4	0.0	0.0	6.53830713137255	0.0
27	2.813022041635137E-4	0.0	0.0	6.6701173070377395	0.0
28	2.813022041635137E-4	0.0	0.0	6.806508690027877	0.0
29	2.813022041635137E-4	0.0	0.0	6.953227882513732	0.0
30	2.813022041635137E-4	0.0	0.0	7.152269284974001	0.0
31	3.2148823332973E-4	0.0	0.0	7.303891173018136	0.0
32	3.2148823332973E-4	0.0	0.0	7.4741593785953935	0.0
33	3.2148823332973E-4	0.0	0.0	7.624334569589544	0.0
34	3.2148823332973E-4	0.0	0.0	7.776237759837842	0.0
35	3.2148823332973E-4	0.0	0.0	7.929105414786128	0.0
36	3.2148823332973E-4	0.0	0.0	8.085228138096879	0.0
37	3.2148823332973E-4	0.0	0.0	8.248584346657546	0.0
38	3.2148823332973E-4	0.0	0.0	8.412864833889039	0.0
39	3.2148823332973E-4	0.0	0.0	8.58457973651628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	10145	0.0	40.963528	1
ACGCTAG	50	1.0822987E-9	40.5	1
GCTACGA	195	0.0	40.384617	10
ACCCGCT	295	0.0	39.66102	34
TATGGGC	1095	0.0	39.246574	4
TATTACG	70	0.0	38.571426	1
CGCGTAA	35	6.2512263E-6	38.571426	31
GACCGAT	1815	0.0	38.429752	9
GCGATCT	245	0.0	37.65306	9
AGGGTAC	1170	0.0	37.5	6
GTAGGGC	1210	0.0	37.190083	4
GGGCGAT	5430	0.0	37.00276	7
TAACGGG	590	0.0	36.991528	3
GGCGATT	1565	0.0	36.80511	8
AGGGCGA	2790	0.0	36.6129	6
CCGACGG	80	0.0	36.5625	2
GGCACCG	1480	0.0	36.334457	8
GGTACCT	1255	0.0	36.215137	8
TAGCATA	1085	0.0	36.08295	30
ATAGGGC	1385	0.0	36.064983	4
>>END_MODULE