FastQCFastQC Report
Thu 26 May 2016
SRR1547669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547669_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3705880
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580924.265977311731627No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT77080.20799378285319545No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC63350.17094455298066857No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT61270.16533185100434986No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT39180.1057238766500804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT624250.044.398081
CGACGGT1650.043.63636428
TATAGCG2000.042.751
CACGACG1700.042.35294326
AATTACG650.041.538461
CGTTCGG10950.041.3013745
GTTTTTT678450.040.917532
CGTTCTG8750.040.371431
CGGTCTA1800.040.031
AGGGCGA41800.039.4019176
ATAACGC2400.039.37499611
ATACTAT30350.039.21746445
TGTACGG3750.039.02
ACGCCGA1850.038.9189212
ACTACGG2200.038.8636362
GCGCGAC7950.038.7735869
ACTCGCG356.252054E-638.5714261
ACGGGAT13100.038.473285
CTTTGCG1350.038.3333361
GGCGCGA8000.038.258
Produced by FastQC (version 0.11.5)