FastQCFastQC Report
Thu 26 May 2016
SRR1547668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547668_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4214442
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168750.4004088797520526No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67050.15909579488814887No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA253.8925824E-545.00000439
CGTTTTT124450.039.756931
TACGGGA11150.039.551574
CCGCGTA403.4611548E-739.37528
GGCGATA21850.038.821518
CATACGA3600.038.7518
TCTAGCG2500.038.6999971
GACCGAT30800.037.9139639
TAACGGG11400.037.697373
GGTACGA900.037.59
GCGATAT5350.037.4299059
ACGGGAT10250.037.3170745
GCGTAAG3500.037.2857171
GGGCGAT113800.037.1902477
AGGGCGA59000.037.144076
TGGGCGA33700.036.9881326
ACGGGTA4150.036.867475
CGTAAGG7400.036.790542
TACGGGT3500.036.642864
CTACGGG8700.036.465523
Produced by FastQC (version 0.11.5)