##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547668_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4214442
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.399682330424763	31.0	31.0	33.0	30.0	34.0
2	31.80585686076591	31.0	31.0	34.0	30.0	34.0
3	31.953509622388918	31.0	31.0	34.0	30.0	34.0
4	35.65460979175891	37.0	35.0	37.0	33.0	37.0
5	35.54023996533824	37.0	35.0	37.0	33.0	37.0
6	35.59123485386677	37.0	35.0	37.0	33.0	37.0
7	35.977093290167474	37.0	35.0	37.0	35.0	37.0
8	35.984850663504204	37.0	35.0	37.0	35.0	37.0
9	37.72244771668468	39.0	37.0	39.0	35.0	39.0
10	37.12123882592286	39.0	37.0	39.0	33.0	39.0
11	36.850606557167	39.0	37.0	39.0	32.0	39.0
12	36.2670092505722	38.0	35.0	39.0	32.0	39.0
13	36.046455022989996	38.0	35.0	39.0	32.0	39.0
14	37.082991295170274	39.0	35.0	41.0	32.0	41.0
15	37.260591556367366	39.0	35.0	41.0	32.0	41.0
16	37.326944587207514	39.0	35.0	41.0	32.0	41.0
17	37.24233196233333	39.0	35.0	41.0	32.0	41.0
18	37.15558619622716	39.0	35.0	41.0	32.0	41.0
19	37.126873261039066	39.0	35.0	41.0	32.0	41.0
20	37.02737562884956	39.0	35.0	41.0	32.0	41.0
21	36.88487016786564	38.0	35.0	41.0	31.0	41.0
22	36.78828869871741	38.0	35.0	41.0	31.0	41.0
23	36.69084448190294	38.0	35.0	40.0	31.0	41.0
24	36.573364635223356	38.0	35.0	40.0	31.0	41.0
25	36.43146589750197	38.0	35.0	40.0	31.0	41.0
26	36.25891897432685	38.0	35.0	40.0	30.0	41.0
27	36.240561858485655	38.0	35.0	40.0	30.0	41.0
28	36.27288476149393	38.0	35.0	40.0	30.0	41.0
29	36.24550842080636	38.0	35.0	40.0	30.0	41.0
30	36.264938513805625	38.0	35.0	40.0	30.0	41.0
31	36.12901446976848	38.0	35.0	40.0	30.0	41.0
32	35.91407996598363	38.0	35.0	40.0	30.0	41.0
33	35.80629060739239	38.0	35.0	40.0	29.0	41.0
34	35.61674095882682	38.0	34.0	40.0	29.0	41.0
35	35.509930377497184	38.0	34.0	40.0	28.0	41.0
36	35.35496442945472	38.0	34.0	40.0	27.0	41.0
37	35.08196743483479	37.0	34.0	40.0	26.0	41.0
38	35.15748846466507	37.0	34.0	40.0	27.0	41.0
39	35.041141389536264	37.0	34.0	40.0	26.0	41.0
40	34.88992777691566	37.0	34.0	40.0	26.0	41.0
41	34.82750005813344	37.0	34.0	40.0	25.0	41.0
42	34.790452686263095	37.0	34.0	40.0	26.0	41.0
43	34.688106041084446	36.0	34.0	40.0	25.0	41.0
44	34.52783334068899	36.0	34.0	40.0	24.0	41.0
45	34.449653121338486	36.0	33.0	40.0	24.0	41.0
46	34.26871789907181	36.0	33.0	40.0	24.0	41.0
47	34.275187082892586	35.0	33.0	40.0	24.0	41.0
48	34.10579241569821	35.0	33.0	40.0	23.0	41.0
49	34.07057731486161	35.0	33.0	40.0	24.0	41.0
50	33.89911119906265	35.0	33.0	39.0	24.0	41.0
51	33.174821482891446	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	6.0
12	10.0
13	23.0
14	43.0
15	133.0
16	339.0
17	977.0
18	2082.0
19	4085.0
20	6808.0
21	10690.0
22	16159.0
23	23641.0
24	33668.0
25	46363.0
26	60679.0
27	72228.0
28	81614.0
29	93632.0
30	110436.0
31	133388.0
32	164189.0
33	211262.0
34	347991.0
35	518168.0
36	338829.0
37	419022.0
38	592023.0
39	925470.0
40	482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.61461802060629	24.506020014037446	27.664445257521635	13.21491670783463
2	31.564961624812966	25.70995163772571	28.608983110931412	14.116103626529918
3	29.426173144629825	26.15387754772755	30.48766598282762	13.932283324815007
4	25.750621315941707	28.96990870914821	29.492896093955025	15.78657388095506
5	23.781179097968366	32.4690908072765	28.641964938656173	15.107765156098957
6	21.973229196178284	40.882992339199355	26.403969968028985	10.739808496593382
7	88.02095271449933	4.274563512797187	6.123491555940264	1.5809922167632156
8	88.37834285060751	3.467932409557422	5.8490305478162945	2.3046941920187773
9	82.67773052755264	5.522747732677304	8.385617835053846	3.4139039047162116
10	47.03006946115286	25.69998590560743	14.145075433473755	13.124869199765948
11	38.58911808490898	24.23023024163104	21.674209776762854	15.506441896697117
12	32.94504942765851	23.020770009410498	26.52960937652007	17.504571186410917
13	23.005275668759946	32.14952299735054	27.781400242309655	17.063801091579858
14	20.238788432727276	33.069360071867166	28.81076545839283	17.88108603701273
15	19.413768180935932	23.3912342369405	39.67735230429082	17.517645277832745
16	21.743376703250394	21.428696847649107	37.57183038703582	19.25609606206468
17	21.92826476197798	21.815604533174263	28.093303929677997	28.162826775169762
18	24.881324740024898	22.86850785940345	31.23533317103427	21.014834229537385
19	29.276544795254033	24.350815600262145	25.238145405726307	21.134494198757512
20	31.333756639669026	24.05125043837357	24.257659732889906	20.357333189067496
21	27.039095567099984	26.31750537793615	26.92097791356483	19.72242114139903
22	26.735923759301944	23.617029253220238	25.581275053731904	24.065771933745914
23	24.192289275780755	28.44848736795998	24.809120638034642	22.55010271822462
24	23.24068049815373	24.439819079251773	32.536454410809306	19.783046011785192
25	22.478278263172207	25.04103746118703	30.967943087127548	21.512741188513214
26	21.36852280800163	29.67211317654864	26.534497330844747	22.424866684604986
27	21.957307752722663	28.561811978904917	27.686963066522214	21.79391720185021
28	20.438553905831427	27.685563118438928	31.945889871067155	19.92999310466249
29	21.56584430394344	25.076344626406062	31.36479277683736	21.99301829281314
30	23.271526811853146	26.728164725009858	29.357077401943126	20.643231061193866
31	28.321258187916694	25.90238517934284	24.557746909318006	21.21860972342246
32	29.10435118101044	25.651130090294277	25.51785028717918	19.7266684415161
33	27.048064726006434	26.158836685853075	24.840322870738284	21.952775717402208
34	23.067656406233613	27.048990115417414	27.67388897510038	22.20946450324859
35	22.819889323426445	27.208346917575327	28.054176567146964	21.917587191851258
36	28.24010865495361	26.450452989980644	24.817116951662875	20.492321403402872
37	23.51072811062532	29.039716289843355	26.495891982853248	20.95366361667808
38	24.06650749968798	30.187531350532286	23.783338339927326	21.96262280985241
39	23.129989687840048	28.795721948480963	25.39344473123607	22.68084363244292
40	25.33488419107441	25.6154195502038	25.011970742508737	24.037725516213058
41	20.62811636748115	25.412213526725484	26.83233035357943	27.127339752213935
42	23.712201994949748	26.195282791885617	25.350259892056886	24.742255321107752
43	23.76986087363404	25.703948470521127	26.203611296584462	24.32257935926037
44	22.942633924016512	27.544642920699825	26.926411610362656	22.58631154492101
45	21.63209269459634	29.83120897143679	24.983188759033816	23.553509574933052
46	24.00215734372427	28.35696872800717	25.908506986215496	21.732366942053062
47	23.663749554508044	26.590708805578533	26.8124226172765	22.933119022636923
48	24.377034966906653	24.689958955420433	28.426871220436773	22.506134857236145
49	23.962863885657935	24.00234716719319	28.680831293917443	23.353957653231436
50	22.099888905814815	29.095643029373758	26.5868411523993	22.21762691241213
51	21.94214560314272	29.167965771032083	25.13720677612837	23.752681849696827
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2383.0
1	3446.0
2	4509.0
3	17403.5
4	30298.0
5	22016.0
6	13734.0
7	14484.0
8	15234.0
9	16554.0
10	17874.0
11	18352.5
12	18831.0
13	19272.5
14	19714.0
15	19264.5
16	18815.0
17	18671.0
18	18527.0
19	18518.0
20	18509.0
21	19539.0
22	20569.0
23	20836.0
24	21103.0
25	23591.5
26	30167.5
27	34255.0
28	37657.0
29	41059.0
30	47044.0
31	53029.0
32	63445.5
33	73862.0
34	81866.5
35	89871.0
36	97147.5
37	104424.0
38	130173.5
39	155923.0
40	190887.0
41	225851.0
42	253727.0
43	281603.0
44	294178.5
45	306754.0
46	304700.5
47	302647.0
48	306615.5
49	310584.0
50	313286.5
51	315989.0
52	306297.5
53	296606.0
54	278094.5
55	259583.0
56	241473.0
57	223363.0
58	207784.0
59	192205.0
60	183283.5
61	174362.0
62	161090.5
63	147819.0
64	129453.5
65	111088.0
66	96125.5
67	81163.0
68	70553.0
69	59943.0
70	51056.5
71	42170.0
72	36594.0
73	31018.0
74	28219.5
75	19862.5
76	14304.0
77	10641.0
78	6978.0
79	5377.5
80	3777.0
81	2432.5
82	1088.0
83	895.5
84	703.0
85	597.5
86	492.0
87	334.5
88	177.0
89	120.5
90	64.0
91	52.0
92	40.0
93	35.5
94	31.0
95	23.0
96	15.0
97	8.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4214442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.894361963047633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.42703504300627	14.57084838637318
2	7.366695043897202	2.6364461517377125
3	2.270407373818405	1.2188247405203683
4	1.0816024092116454	0.774183400421502
5	0.6535223781268166	0.584718299257647
6	0.46570115141601537	0.500005498202774
7	0.3641788063487948	0.4561723166053175
8	0.2914237631770352	0.4171873842338673
9	0.23469843317532224	0.3779800843859427
>10	2.878266303405865	12.55729596970866
>50	1.3533326177376326	17.707974232517138
>100	1.6063979250685863	46.70015905575833
>500	0.00498667619175061	0.5844304621642188
>1k	0.0014825253543042354	0.3450920432099949
>5k	1.3477503220947593E-4	0.1617053707263272
>10k+	1.3477503220947593E-4	0.40697660417699805
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16875	0.4004088797520526	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6705	0.15909579488814887	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.372793361493645E-5	0.0	0.0	0.02954127735059588	0.0
2	2.372793361493645E-5	0.0	0.0	0.10143691620385333	0.0
3	2.372793361493645E-5	0.0	0.0	0.16009236809997623	0.0
4	2.372793361493645E-5	0.0	0.0	0.283026792158962	0.0
5	2.372793361493645E-5	0.0	0.0	0.4751518706391024	0.0
6	2.372793361493645E-5	0.0	0.0	0.9145932011877255	0.0
7	2.372793361493645E-5	0.0	0.0	1.121239775040207	0.0
8	2.372793361493645E-5	0.0	0.0	1.6963811579326515	0.0
9	2.372793361493645E-5	0.0	0.0	1.9872856240517724	0.0
10	2.372793361493645E-5	0.0	0.0	2.4017414405038675	0.0
11	2.372793361493645E-5	0.0	0.0	2.699811742574699	0.0
12	2.372793361493645E-5	0.0	0.0	2.9603681816003165	0.0
13	2.372793361493645E-5	0.0	0.0	3.1012883793394237	0.0
14	2.372793361493645E-5	0.0	0.0	3.1727331874539977	0.0
15	2.372793361493645E-5	0.0	0.0	3.238910394306055	0.0
16	2.372793361493645E-5	0.0	0.0	3.355106085218399	0.0
17	2.372793361493645E-5	0.0	0.0	3.482074257991924	0.0
18	2.372793361493645E-5	0.0	0.0	3.6733925867291566	0.0
19	2.372793361493645E-5	0.0	0.0	3.755301413567917	0.0
20	9.49117344597458E-5	0.0	0.0	3.8437591500844004	0.0
21	9.49117344597458E-5	0.0	0.0	3.955399077742676	0.0
22	9.49117344597458E-5	0.0	0.0	4.074560760356887	0.0
23	9.49117344597458E-5	0.0	0.0	4.209762526094795	0.0
24	9.49117344597458E-5	0.0	0.0	4.318104270980595	0.0
25	9.49117344597458E-5	0.0	0.0	4.413514292046254	0.0
26	9.49117344597458E-5	0.0	0.0	4.506432880082345	0.0
27	9.49117344597458E-5	0.0	0.0	4.602317459820304	0.0
28	1.6609553530455516E-4	0.0	0.0	4.696873275275825	0.0
29	1.6609553530455516E-4	0.0	0.0	4.799235580890661	0.0
30	1.898234689194916E-4	0.0	0.0	4.950999444291795	0.0
31	2.1355140253442805E-4	0.0	0.0	5.06691040000076	0.0
32	2.372793361493645E-4	0.0	0.0	5.192004066018704	0.0
33	2.372793361493645E-4	0.0	0.0	5.307416735121755	0.0
34	2.372793361493645E-4	0.0	0.0	5.4241818964408575	0.0
35	2.372793361493645E-4	0.0	0.0	5.554282156451554	0.0
36	2.372793361493645E-4	0.0	0.0	5.672613361389242	0.0
37	2.372793361493645E-4	0.0	0.0	5.798181586079486	0.0
38	2.372793361493645E-4	0.0	0.0	5.935447682041893	0.0
39	2.372793361493645E-4	0.0	0.0	6.111390309796647	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	25	3.8925824E-5	45.000004	39
CGTTTTT	12445	0.0	39.75693	1
TACGGGA	1115	0.0	39.55157	4
CCGCGTA	40	3.4611548E-7	39.375	28
GGCGATA	2185	0.0	38.82151	8
CATACGA	360	0.0	38.75	18
TCTAGCG	250	0.0	38.699997	1
GACCGAT	3080	0.0	37.913963	9
TAACGGG	1140	0.0	37.69737	3
GGTACGA	90	0.0	37.5	9
GCGATAT	535	0.0	37.429905	9
ACGGGAT	1025	0.0	37.317074	5
GCGTAAG	350	0.0	37.285717	1
GGGCGAT	11380	0.0	37.190247	7
AGGGCGA	5900	0.0	37.14407	6
TGGGCGA	3370	0.0	36.988132	6
ACGGGTA	415	0.0	36.86747	5
CGTAAGG	740	0.0	36.79054	2
TACGGGT	350	0.0	36.64286	4
CTACGGG	870	0.0	36.46552	3
>>END_MODULE