FastQCFastQC Report
Thu 26 May 2016
SRR1547667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547667_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences859626
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54810.637602864501539No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT302.1648611E-645.00000421
TCTAGCG207.0322945E-445.01
CGTTTTT37650.040.4581681
ACGTTAG451.9274012E-840.01
TACGTAG403.456862E-739.3751
GTACGGG2600.038.942313
TACGGGT1050.038.571434
CGGGTAT700.038.5714266
TCATACG356.2464514E-638.5714261
TAATGCG356.2464514E-638.5714261
TATGGGT2000.038.254
ACGTAGG950.037.8947372
GGGCGAT21450.037.7622387
CGATCGA301.13968235E-437.50000410
TCGTAAG900.037.51
AGGGCGA10450.037.4641156
GTAGCAT5050.037.42574329
ACGGGCT1450.037.241385
AGGGATT5600.036.9642836
TACGGGA2200.036.818184
Produced by FastQC (version 0.11.5)