##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547667_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	859626
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43033249343319	31.0	31.0	33.0	30.0	34.0
2	31.84757441026679	31.0	31.0	34.0	30.0	34.0
3	31.98985605367916	33.0	31.0	34.0	30.0	34.0
4	35.698272271894986	37.0	35.0	37.0	33.0	37.0
5	35.587799810615316	37.0	35.0	37.0	33.0	37.0
6	35.6319841419408	37.0	35.0	37.0	33.0	37.0
7	35.985185417844505	37.0	35.0	37.0	35.0	37.0
8	35.98347071866137	37.0	35.0	37.0	35.0	37.0
9	37.733957558286974	39.0	37.0	39.0	35.0	39.0
10	37.155382689681325	39.0	37.0	39.0	33.0	39.0
11	36.89316051399097	39.0	37.0	39.0	32.0	39.0
12	36.38098545181276	38.0	35.0	39.0	32.0	39.0
13	36.20376768501651	39.0	35.0	39.0	32.0	39.0
14	37.23291873442637	39.0	35.0	41.0	32.0	41.0
15	37.38671701414336	39.0	35.0	41.0	32.0	41.0
16	37.45065412167617	39.0	35.0	41.0	32.0	41.0
17	37.36618250262323	39.0	35.0	41.0	32.0	41.0
18	37.254148897311154	39.0	35.0	41.0	32.0	41.0
19	37.18274109903609	39.0	35.0	41.0	32.0	41.0
20	37.07043528232045	39.0	35.0	41.0	32.0	41.0
21	36.94130587022728	39.0	35.0	41.0	31.0	41.0
22	36.864922652409305	38.0	35.0	41.0	31.0	41.0
23	36.75753176381357	38.0	35.0	41.0	31.0	41.0
24	36.627247198200145	38.0	35.0	41.0	31.0	41.0
25	36.47036269261283	38.0	35.0	40.0	31.0	41.0
26	36.30383445824114	38.0	35.0	40.0	30.0	41.0
27	36.2697952365331	38.0	35.0	40.0	30.0	41.0
28	36.273613175962566	38.0	35.0	40.0	30.0	41.0
29	36.24206457226747	38.0	35.0	40.0	30.0	41.0
30	36.24266832320102	38.0	35.0	40.0	30.0	41.0
31	36.11632151656651	38.0	35.0	40.0	30.0	41.0
32	35.907203830503036	38.0	35.0	40.0	30.0	41.0
33	35.75528776467906	38.0	35.0	40.0	29.0	41.0
34	35.487686505526824	38.0	34.0	40.0	27.0	41.0
35	35.33617410362181	38.0	34.0	40.0	27.0	41.0
36	35.16761126350296	38.0	34.0	40.0	26.0	41.0
37	34.92574445165688	37.0	34.0	40.0	25.0	41.0
38	34.976379262609555	37.0	34.0	40.0	26.0	41.0
39	34.839736117800065	37.0	34.0	40.0	25.0	41.0
40	34.67791690805071	37.0	34.0	40.0	24.0	41.0
41	34.60021567518898	37.0	34.0	40.0	24.0	41.0
42	34.55163291943241	36.0	33.0	40.0	24.0	41.0
43	34.45127764865185	36.0	33.0	40.0	24.0	41.0
44	34.27267788549904	36.0	33.0	40.0	23.0	41.0
45	34.191814812488225	36.0	33.0	40.0	23.0	41.0
46	34.00867935590594	35.0	33.0	40.0	23.0	41.0
47	34.0025383131734	35.0	33.0	40.0	23.0	41.0
48	33.81845825975483	35.0	33.0	40.0	23.0	41.0
49	33.78018812832558	35.0	33.0	39.0	23.0	41.0
50	33.63389194835894	35.0	33.0	39.0	23.0	41.0
51	32.923363183523996	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	3.0
14	9.0
15	30.0
16	92.0
17	232.0
18	428.0
19	872.0
20	1450.0
21	2358.0
22	3465.0
23	5110.0
24	7294.0
25	10315.0
26	13446.0
27	15992.0
28	17861.0
29	19778.0
30	22725.0
31	26866.0
32	32835.0
33	42032.0
34	69228.0
35	95452.0
36	72062.0
37	90855.0
38	124423.0
39	184306.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.683718268177095	24.27986124198198	27.196827457522225	14.839593032318707
2	32.6821198986536	25.275177809884763	28.043125731422734	13.999576560038902
3	29.443734833520622	25.80250015704504	30.542352139186114	14.211412870248225
4	25.800638882490755	28.450977518129978	30.472554343400503	15.275829255978762
5	23.763241223508828	31.939355021835077	29.1683825291464	15.129021225509698
6	22.267474459823227	40.628831608164475	26.411718584593764	10.691975347418529
7	87.03738602601597	4.0291940913839275	7.14857391470244	1.7848459678976671
8	87.47676315048636	3.280961720562198	7.176958351655255	2.065316777296173
9	81.90271117904763	5.209358488458935	9.418747222629376	3.4691831098640575
10	46.55152356955234	24.013582651059878	16.004518243980524	13.430375535407258
11	37.44628477966	24.263575089632003	21.874978188188816	16.415161942519187
12	31.23497893269864	21.906968844590555	27.740784946011406	19.1172672766994
13	24.5680098089169	27.53965096448921	29.329615437411157	18.562723789182737
14	21.54157738365289	28.949682769018153	30.81212061989749	18.696619227431466
15	20.72261657976841	22.021553559338596	37.8507630062376	19.4050668546554
16	24.58964712561044	20.55126299111474	34.579223988106456	20.279865895168363
17	24.37943943063611	21.26343316744724	28.393045347627922	25.964082054288724
18	26.968007017005068	21.474106181060133	30.329934180678574	21.22795262125622
19	29.809242624117932	23.32328245074021	25.867761096104587	20.99971382903728
20	31.236840207252918	23.30176146370631	24.669798261104248	20.79160006793652
21	27.873051769025135	24.405031955757504	27.34793968539807	20.373976589819293
22	27.652839723321538	21.954780334703695	26.394501794966647	23.997878147008116
23	25.357306549592494	25.328456794001113	26.055633496427514	23.258603159978875
24	24.964926607617734	23.085969945069134	30.800254994613933	21.148848452699198
25	24.762978318478034	23.588165085746592	29.10858908409006	22.540267511685315
26	23.389241367757606	26.410206299018412	26.523395057850742	23.677157275373244
27	23.464506657546423	25.188046894812395	28.626053655892214	22.721392791748972
28	22.323080037132428	25.271106271797272	30.626574812767416	21.77923887830289
29	23.855606973265118	24.1523639350136	29.713968632870575	22.27806045885071
30	24.74192265008271	24.524386186550895	29.183970703538513	21.549720459827878
31	28.450046880852835	23.988804433555988	25.606833669526054	21.954315016065127
32	29.449434986843116	23.8590968630544	25.477358758343744	21.214109391758743
33	28.390602424775423	24.608376200812913	24.877563033226078	22.123458341185586
34	24.522292252677328	26.029575652667557	27.306526326565272	22.141605768089846
35	25.23853396709732	26.171381507771983	27.25254936449107	21.337535160639625
36	28.19830949738607	26.711383787833316	24.568242468236186	20.522064246544428
37	25.296349807939734	28.15049800727293	25.08451349773041	21.46863868705693
38	26.36623368767348	27.9228408633522	23.812913988176255	21.898011460798067
39	25.268430689625486	27.180890294151176	24.48983627763702	23.060842738586317
40	27.317344984912044	24.65165083419999	24.739130738251287	23.291873442636682
41	22.6628789729487	24.074655722372288	26.203139504854438	27.059325799824574
42	25.32229132204005	25.084862486709337	24.642344461428575	24.95050172982204
43	24.873491495138584	24.309176316211932	26.295970573249296	24.521361615400185
44	24.179003427071773	25.53785018135794	27.12377243126662	23.159373960303668
45	23.213001933398942	27.156112076647286	25.48619981247659	24.144686177477183
46	24.78868717325907	26.022828532408283	26.019222312959357	23.169261981373296
47	24.082798798547277	25.63556709545779	27.24487160695465	23.036762499040282
48	24.887567383955346	24.913159909076736	27.495329364165343	22.703943342802567
49	24.86639538590038	24.124328487039712	27.684597720403993	23.32467840665592
50	23.354458799524444	27.05769718458958	26.73011286303579	22.857731152850196
51	23.37074495187442	27.434954270810795	25.417914302266336	23.77638647504845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	524.0
1	790.5
2	1057.0
3	5209.5
4	9362.0
5	6555.5
6	3749.0
7	3836.0
8	3923.0
9	4109.0
10	4295.0
11	4449.0
12	4603.0
13	4611.0
14	4619.0
15	4432.0
16	4245.0
17	4106.0
18	3967.0
19	3845.0
20	3723.0
21	3721.0
22	3719.0
23	3957.0
24	4195.0
25	4304.0
26	5041.0
27	5669.0
28	6394.0
29	7119.0
30	8896.0
31	10673.0
32	12116.0
33	13559.0
34	15058.0
35	16557.0
36	18386.0
37	20215.0
38	22208.0
39	24201.0
40	29504.5
41	34808.0
42	40582.0
43	46356.0
44	47777.0
45	49198.0
46	50112.0
47	51026.0
48	52503.0
49	53980.0
50	56461.0
51	58942.0
52	58254.5
53	57567.0
54	56127.5
55	54688.0
56	53049.0
57	51410.0
58	50311.0
59	49212.0
60	47218.5
61	45225.0
62	42270.0
63	39315.0
64	36468.5
65	33622.0
66	29612.5
67	25603.0
68	21898.5
69	18194.0
70	15430.5
71	12667.0
72	10819.5
73	8972.0
74	7678.5
75	5260.0
76	4135.0
77	2996.0
78	1857.0
79	1549.5
80	1242.0
81	862.5
82	483.0
83	353.5
84	224.0
85	144.0
86	64.0
87	46.5
88	29.0
89	24.0
90	19.0
91	16.0
92	13.0
93	7.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	859626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.319468904445717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03933771742741	21.121551221029762
2	5.895596138017421	3.4571149528354406
3	3.095774376435581	2.722993816952485
4	2.130228644269328	2.4982868998005645
5	1.741567972637837	2.553092400936681
6	1.479582976512637	2.60283522428457
7	1.2869044735016237	2.6411948985676057
8	1.1117169516631797	2.607596047586702
9	1.0586000358988006	2.7933831751302
>10	10.075650006314023	54.243348260999134
>50	0.06867137021387458	1.2872935268035626
>100	0.015570832859070379	0.7329039103774545
>500	3.992521245915459E-4	0.09680746428400457
>1k	0.0	0.0
>5k	3.992521245915459E-4	0.6415982004118852
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5481	0.637602864501539	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019543382819970544	0.0
2	0.0	0.0	0.0	0.0754979491081005	0.0
3	0.0	0.0	0.0	0.12424007649838419	0.0
4	0.0	0.0	0.0	0.19869105866970055	0.0
5	0.0	0.0	0.0	0.3183942784420201	0.0
6	0.0	0.0	0.0	0.6563319397040108	0.0
7	0.0	0.0	0.0	0.8212873970773337	0.0
8	0.0	0.0	0.0	1.2144816466695982	0.0
9	0.0	0.0	0.0	1.441208153313185	0.0
10	0.0	0.0	0.0	1.7793784738944611	0.0
11	0.0	0.0	0.0	2.0259973523369466	0.0
12	0.0	0.0	0.0	2.233762124458776	0.0
13	0.0	0.0	0.0	2.3597471458518005	0.0
14	0.0	0.0	0.0	2.4376880178123974	0.0
15	0.0	0.0	0.0	2.4963181662723093	0.0
16	0.0	0.0	0.0	2.593337102414306	0.0
17	0.0	0.0	0.0	2.690821357194873	0.0
18	0.0	0.0	0.0	2.830998597064305	0.0
19	0.0	0.0	0.0	2.8980044810184893	0.0
20	1.1632965964268182E-4	0.0	0.0	2.976759660596585	0.0
21	1.1632965964268182E-4	0.0	0.0	3.0585394113253903	0.0
22	2.3265931928536363E-4	0.0	0.0	3.1539297322323896	0.0
23	2.3265931928536363E-4	0.0	0.0	3.25502020646188	0.0
24	2.3265931928536363E-4	0.0	0.0	3.3398245283413948	0.0
25	2.3265931928536363E-4	0.0	0.0	3.4186960375791333	0.0
26	2.3265931928536363E-4	0.0	0.0	3.4917510638347373	0.0
27	2.3265931928536363E-4	0.0	0.0	3.58469846188924	0.0
28	2.3265931928536363E-4	0.0	0.0	3.664849597383048	0.0
29	2.3265931928536363E-4	0.0	0.0	3.754772424286841	0.0
30	2.3265931928536363E-4	0.0	0.0	3.8756389406555876	0.0
31	2.3265931928536363E-4	0.0	0.0	3.9801029750147157	0.0
32	2.3265931928536363E-4	0.0	0.0	4.107367622663809	0.0
33	2.3265931928536363E-4	0.0	0.0	4.218462447622571	0.0
34	2.3265931928536363E-4	0.0	0.0	4.319320262532776	0.0
35	2.3265931928536363E-4	0.0	0.0	4.444258316989016	0.0
36	2.3265931928536363E-4	0.0	0.0	4.549420329306001	0.0
37	2.3265931928536363E-4	0.0	0.0	4.658886539029764	0.0
38	2.3265931928536363E-4	0.0	0.0	4.789524746808496	0.0
39	2.3265931928536363E-4	0.0	0.0	5.015902264473155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	30	2.1648611E-6	45.000004	21
TCTAGCG	20	7.0322945E-4	45.0	1
CGTTTTT	3765	0.0	40.458168	1
ACGTTAG	45	1.9274012E-8	40.0	1
TACGTAG	40	3.456862E-7	39.375	1
GTACGGG	260	0.0	38.94231	3
TACGGGT	105	0.0	38.57143	4
CGGGTAT	70	0.0	38.571426	6
TCATACG	35	6.2464514E-6	38.571426	1
TAATGCG	35	6.2464514E-6	38.571426	1
TATGGGT	200	0.0	38.25	4
ACGTAGG	95	0.0	37.894737	2
GGGCGAT	2145	0.0	37.762238	7
CGATCGA	30	1.13968235E-4	37.500004	10
TCGTAAG	90	0.0	37.5	1
AGGGCGA	1045	0.0	37.464115	6
GTAGCAT	505	0.0	37.425743	29
ACGGGCT	145	0.0	37.24138	5
AGGGATT	560	0.0	36.964283	6
TACGGGA	220	0.0	36.81818	4
>>END_MODULE