FastQCFastQC Report
Thu 26 May 2016
SRR1547666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547666_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7219605
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363840.5039610892839705No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC100390.13905192874125386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT232200.040.5038761
CATAGCG2550.039.7058831
AGGGAAC110850.039.539926
TACGGGA22050.039.4897964
TAACGCG3150.039.2857131
TATAGCG4450.038.9325831
CGTTCGG13900.038.6870545
CGTAAGG9450.038.571432
GCGAGAC92850.038.33602521
TACGAAC1000.038.2539
CGAGACA94250.037.6233422
ACGGGAC19050.037.5590555
CGAATAT4500.037.514
ATAGCGG14050.037.3131682
TAGGGCG29500.037.296615
TAAGGGA88300.037.2791634
AGCGGGA62250.037.192774
CGGTCTA1150.037.17391231
GGGCGAT171950.037.1227077
AGACACG95300.037.04354524