##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547666_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	7219605
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432524078533383	31.0	31.0	33.0	30.0	34.0
2	31.82840072275422	31.0	31.0	34.0	30.0	34.0
3	31.97320158097292	33.0	31.0	34.0	30.0	34.0
4	35.659007660391396	37.0	35.0	37.0	33.0	37.0
5	35.545138411311974	37.0	35.0	37.0	33.0	37.0
6	35.58778700496772	37.0	35.0	37.0	33.0	37.0
7	36.012607199424345	37.0	35.0	37.0	35.0	37.0
8	36.028078682975035	37.0	35.0	37.0	35.0	37.0
9	37.75701496134484	39.0	38.0	39.0	35.0	39.0
10	37.152223563477506	39.0	37.0	39.0	33.0	39.0
11	36.887687622799305	39.0	37.0	39.0	32.0	39.0
12	36.360997589203286	38.0	35.0	39.0	32.0	39.0
13	36.15406521547924	38.0	35.0	39.0	32.0	39.0
14	37.21550431083141	39.0	35.0	41.0	32.0	41.0
15	37.39602263558741	39.0	35.0	41.0	32.0	41.0
16	37.469374155511275	39.0	35.0	41.0	32.0	41.0
17	37.34099760305446	39.0	35.0	41.0	32.0	41.0
18	37.274935124567065	39.0	35.0	41.0	32.0	41.0
19	37.244673219656754	39.0	35.0	41.0	32.0	41.0
20	37.15800864451725	39.0	35.0	41.0	32.0	41.0
21	37.021531094845216	39.0	35.0	41.0	32.0	41.0
22	36.92679336334883	39.0	35.0	41.0	31.0	41.0
23	36.842730592601676	38.0	35.0	41.0	31.0	41.0
24	36.7380455024894	38.0	35.0	41.0	31.0	41.0
25	36.60282813256404	38.0	35.0	40.0	31.0	41.0
26	36.449477222091794	38.0	35.0	40.0	31.0	41.0
27	36.43112372491293	38.0	35.0	40.0	31.0	41.0
28	36.468166610223136	38.0	35.0	40.0	31.0	41.0
29	36.45792325203387	38.0	35.0	41.0	31.0	41.0
30	36.46599294559744	38.0	35.0	41.0	31.0	41.0
31	36.33289909905043	38.0	35.0	41.0	30.0	41.0
32	36.10635083221312	38.0	35.0	40.0	30.0	41.0
33	36.007418965442014	38.0	35.0	40.0	30.0	41.0
34	35.85128715490667	38.0	35.0	40.0	29.0	41.0
35	35.74793994962328	38.0	35.0	40.0	29.0	41.0
36	35.59616724183664	38.0	35.0	40.0	28.0	41.0
37	35.33464406986255	38.0	34.0	40.0	27.0	41.0
38	35.38909801852041	38.0	34.0	40.0	27.0	41.0
39	35.314641590502525	38.0	34.0	40.0	27.0	41.0
40	35.15329079638013	37.0	34.0	40.0	26.0	41.0
41	35.13129665681156	37.0	34.0	40.0	26.0	41.0
42	35.10309843821096	37.0	34.0	40.0	27.0	41.0
43	35.00158443571359	37.0	34.0	40.0	26.0	41.0
44	34.84480286663882	37.0	34.0	40.0	26.0	41.0
45	34.74819024032478	37.0	34.0	40.0	26.0	41.0
46	34.55061945909783	36.0	34.0	40.0	24.0	41.0
47	34.56386658272856	36.0	34.0	40.0	25.0	41.0
48	34.42111334345854	36.0	34.0	40.0	24.0	41.0
49	34.403629422939346	36.0	34.0	40.0	24.0	41.0
50	34.26090153131646	36.0	33.0	40.0	24.0	41.0
51	33.58539199859272	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	2.0
12	12.0
13	20.0
14	58.0
15	235.0
16	583.0
17	1461.0
18	3257.0
19	6184.0
20	10480.0
21	16896.0
22	25659.0
23	37542.0
24	53966.0
25	74879.0
26	97974.0
27	117673.0
28	133273.0
29	151689.0
30	180054.0
31	216622.0
32	268174.0
33	344284.0
34	559111.0
35	881576.0
36	564751.0
37	699788.0
38	1019489.0
39	1752750.0
40	1157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.03774860258975	24.375807263693787	27.9440357194057	13.642408414310756
2	28.987084473458037	26.793584967598644	28.845774803469165	15.373555755474158
3	28.824790275922297	26.424672817972727	30.76100423776647	13.989532668338503
4	24.177042926863727	29.953757857943753	29.664309889529967	16.204889325662553
5	23.73560603384811	32.6545427346787	28.629336369510522	14.980514861962671
6	21.658594895427104	40.644356581835154	25.900959955565433	11.796088567172305
7	88.46597563163081	3.9139952947564303	6.009359237797636	1.6106698358151175
8	88.75525738596502	3.2501085585707252	5.500162405006922	2.4944716504573314
9	82.9163645379491	5.903854850784772	8.034151452884194	3.145629158381934
10	41.710716860548466	33.84651930403395	12.830147909754066	11.612615925663523
11	35.86135252551906	25.031466402940328	24.709440474929032	14.39774059661159
12	31.46882689565426	24.130710752181038	26.230562475370885	18.169899876793814
13	22.222240690453287	34.48728843198485	26.834598291734796	16.455872585827063
14	17.914428836480667	35.61106182401946	28.21999264502698	18.254516694472898
15	17.301098882833617	25.26609142743959	39.37449486502378	18.058314824703015
16	20.75264505468097	23.312106410253747	38.02620780499764	17.909040730067645
17	19.167530633601146	23.529791449809235	28.877410883282394	28.42526703330722
18	23.933345383854103	23.3188796339966	30.96933973534563	21.778435246803667
19	26.29059346044555	26.78333509935793	26.16144512061255	20.764626319583968
20	29.970933312833598	24.891998939000125	24.794334316074078	20.3427334320922
21	24.76828025910005	28.46456281195439	26.63809169615235	20.129065232793206
22	24.544168275134165	24.57767426334266	26.20499320946229	24.673164252060882
23	21.606943870197885	31.360995511527296	24.802922043519	22.229138574755822
24	21.674440637680316	25.65284388827367	33.32909487430407	19.343620599741953
25	20.77410052211998	27.120721978557	30.962386446349903	21.14279105297312
26	20.029683064378176	32.19973945943026	26.75812596395509	21.012451512236474
27	20.85568393284674	29.519703640296107	27.400917363207544	22.223695063649604
28	18.161270595828995	30.401718653582847	32.10758760347692	19.329423147111232
29	19.175328844168067	26.83331013261806	31.69580607249289	22.295554950720987
30	21.38054644263779	27.658119800182973	30.240726466337147	20.72060729084209
31	26.388992195556405	27.692456858789367	24.356637239848993	21.561913705805235
32	25.09615138224321	28.64087439686797	26.090097726953204	20.17287649393561
33	23.181379036664747	28.7155876256388	24.888272973382893	23.214760364313562
34	19.60442434177493	30.20260803742033	28.332644237461746	21.860323383342994
35	19.238961134300283	29.25671141288201	28.465463138218784	23.03886431459893
36	23.953415733963286	29.765395752260687	25.696364274776805	20.584824238999225
37	21.074283149839914	30.294302804654826	26.3459981536386	22.285415891866663
38	20.870255367156513	31.069802295277928	25.21618565004595	22.84375668751961
39	21.935618361392347	28.76227993082724	25.297824465465908	24.004277242314505
40	23.802451796185526	26.79271234368085	25.848976502177056	23.555859357956564
41	18.318564519804063	26.84031882630698	26.739385326482545	28.101731327406416
42	23.092662271689377	27.410059137584398	24.48832034439557	25.008958246330653
43	22.778905494137145	26.97626809222942	26.046591191623364	24.198235222010066
44	22.236022607885058	29.53949696693933	26.50866633285339	21.715814092322226
45	20.76929416498548	30.562253752109708	24.56977909456265	24.09867298834216
46	22.913982136141797	28.71134916660953	25.56385009983233	22.81081859741634
47	22.279626101427986	27.63671973743716	27.47284650614542	22.610807654989433
48	23.620308867313376	25.557201536649167	28.360609756350936	22.46187983968652
49	22.201463930505895	25.544873992413713	28.354016597860966	23.89964547921943
50	20.526275329467474	29.327504759609425	27.34969572435057	22.796524186572533
51	20.273200542134923	29.607104543808145	25.296439348136087	24.823255565920853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5002.0
1	6372.0
2	7742.0
3	34104.0
4	60466.0
5	42331.0
6	24196.0
7	25542.5
8	26889.0
9	29317.0
10	31745.0
11	33707.5
12	35670.0
13	36173.5
14	36677.0
15	35973.0
16	35269.0
17	35106.0
18	34943.0
19	34479.5
20	34016.0
21	34938.5
22	35861.0
23	36699.5
24	37538.0
25	43967.0
26	55231.0
27	60066.0
28	76795.5
29	93525.0
30	102754.0
31	111983.0
32	129077.0
33	146171.0
34	169477.0
35	192783.0
36	205545.0
37	218307.0
38	247048.5
39	275790.0
40	344973.5
41	414157.0
42	472570.0
43	530983.0
44	553777.0
45	576571.0
46	585303.0
47	594035.0
48	595195.0
49	596355.0
50	596172.5
51	595990.0
52	555561.5
53	515133.0
54	460875.5
55	406618.0
56	365847.5
57	325077.0
58	298443.0
59	271809.0
60	247720.0
61	223631.0
62	205362.0
63	187093.0
64	162091.0
65	137089.0
66	114673.5
67	92258.0
68	74814.0
69	57370.0
70	49004.0
71	40638.0
72	36685.0
73	32732.0
74	32538.0
75	26637.0
76	20930.0
77	14313.5
78	7697.0
79	5455.5
80	3214.0
81	2547.0
82	1880.0
83	1227.5
84	575.0
85	430.5
86	286.0
87	171.5
88	57.0
89	46.5
90	36.0
91	21.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	7219605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.862176451739753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82944512655509	13.46098189768448
2	8.762625458683523	2.9551387332965713
3	2.8697408447379424	1.4517022948420766
4	1.3675029582284364	0.9223630471969595
5	0.8049464625802814	0.6786574643116215
6	0.5334129238100336	0.5396701705753918
7	0.42348911703721187	0.4998663751811054
8	0.30089553899274013	0.4059002937629539
9	0.2632048545802448	0.3994386030797931
>10	2.406559571347919	9.279257566159252
>50	0.8516343205971632	10.421231621447864
>100	1.5608117276942617	54.36856617375878
>500	0.02005020402051188	2.1749475590858505
>1k	0.0053467210678338145	1.6288616465672379
>5k	1.670850333698067E-4	0.15945173294958456
>10k+	1.670850333698067E-4	0.6539648201005888
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36384	0.5039610892839705	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10039	0.13905192874125386	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7702346596524325E-5	0.0	0.0	0.06224717280239016	0.0
2	2.7702346596524325E-5	0.0	0.0	0.2030166470326285	0.0
3	2.7702346596524325E-5	0.0	0.0	0.3064987627439451	0.0
4	2.7702346596524325E-5	0.0	0.0	0.4977696148196473	0.0
5	2.7702346596524325E-5	0.0	0.0	0.8197539893110496	0.0
6	2.7702346596524325E-5	0.0	0.0	1.5131852781419481	0.0
7	2.7702346596524325E-5	0.0	0.0	1.85701295292471	0.0
8	2.7702346596524325E-5	0.0	0.0	2.7521588784982005	0.0
9	2.7702346596524325E-5	0.0	0.0	3.231631093390843	0.0
10	2.7702346596524325E-5	0.0	0.0	3.9277356586683068	0.0
11	2.7702346596524325E-5	0.0	0.0	4.424092453811531	0.0
12	2.7702346596524325E-5	0.0	0.0	4.8605567756130705	0.0
13	2.7702346596524325E-5	0.0	0.0	5.119629120983766	0.0
14	2.7702346596524325E-5	0.0	0.0	5.256187838531332	0.0
15	2.7702346596524325E-5	0.0	0.0	5.37895078747383	0.0
16	2.7702346596524325E-5	0.0	0.0	5.565900073480474	0.0
17	2.7702346596524325E-5	0.0	0.0	5.764013405165518	0.0
18	2.7702346596524325E-5	0.0	0.0	6.056342417625341	0.0
19	4.155351989478649E-5	0.0	0.0	6.200699345739829	0.0
20	1.108093863860973E-4	0.0	0.0	6.341011731251225	0.0
21	1.108093863860973E-4	0.0	0.0	6.501740746204259	0.0
22	1.2466055968435948E-4	0.0	0.0	6.672054773079691	0.0
23	1.2466055968435948E-4	0.0	0.0	6.869046159727575	0.0
24	1.2466055968435948E-4	0.0	0.0	7.023057355630952	0.0
25	1.2466055968435948E-4	0.0	0.0	7.151333071546158	0.0
26	1.3851173298262164E-4	0.0	0.0	7.286880653442951	0.0
27	1.3851173298262164E-4	0.0	0.0	7.431362242117124	0.0
28	1.3851173298262164E-4	0.0	0.0	7.582090709948813	0.0
29	1.3851173298262164E-4	0.0	0.0	7.72948104501562	0.0
30	1.523629062808838E-4	0.0	0.0	7.963593576102848	0.0
31	1.523629062808838E-4	0.0	0.0	8.13263329503484	0.0
32	1.523629062808838E-4	0.0	0.0	8.398465012975086	0.0
33	1.523629062808838E-4	0.0	0.0	8.571438465123784	0.0
34	1.523629062808838E-4	0.0	0.0	8.725906749746004	0.0
35	1.6621407957914596E-4	0.0	0.0	8.892231638711536	0.0
36	1.6621407957914596E-4	0.0	0.0	9.050522847164077	0.0
37	1.6621407957914596E-4	0.0	0.0	9.212803193526515	0.0
38	1.6621407957914596E-4	0.0	0.0	9.372188644669619	0.0
39	1.6621407957914596E-4	0.0	0.0	9.541477684720979	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	23220	0.0	40.503876	1
CATAGCG	255	0.0	39.705883	1
AGGGAAC	11085	0.0	39.53992	6
TACGGGA	2205	0.0	39.489796	4
TAACGCG	315	0.0	39.285713	1
TATAGCG	445	0.0	38.932583	1
CGTTCGG	1390	0.0	38.68705	45
CGTAAGG	945	0.0	38.57143	2
GCGAGAC	9285	0.0	38.336025	21
TACGAAC	100	0.0	38.25	39
CGAGACA	9425	0.0	37.62334	22
ACGGGAC	1905	0.0	37.559055	5
CGAATAT	450	0.0	37.5	14
ATAGCGG	1405	0.0	37.313168	2
TAGGGCG	2950	0.0	37.29661	5
TAAGGGA	8830	0.0	37.279163	4
AGCGGGA	6225	0.0	37.19277	4
CGGTCTA	115	0.0	37.173912	31
GGGCGAT	17195	0.0	37.122707	7
AGACACG	9530	0.0	37.043545	24
>>END_MODULE